Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1987 Dec;25(12):2309–2313. doi: 10.1128/jcm.25.12.2309-2313.1987

Restriction endonuclease analysis of members of the Mycobacterium avium-M. intracellulare-M. scrofulaceum serocomplex.

B J Wards 1, D M Collins 1, G W de Lisle 1
PMCID: PMC269477  PMID: 2828414

Abstract

Organisms belonging to the Mycobacterium avium-M. intracellulare-M. scrofulaceum (MAIS) serocomplex were subjected to restriction endonuclease analysis (REA) with the enzymes BstEII, PvuII, and BclI. Substantial genetic heterogeneity was observed between members of an authenticated collection of the 31 serotypes. Serotypes 2 and 3 were indistinguishable, however, as were serotypes 5 and 10. No direct correlation could be made between restriction pattern and species identification. REA of serotype 2 and serotype 8 isolates from various geographic locations and animal origins showed that, within limits, the restriction pattern could be used as an index of serotype. Some isolates that were unable to be classified serologically exhibited restriction patterns identical to those of strains that were able to be classified by seroagglutination. The difficulty of interpreting much of the epidemiological data concerning MAIS organisms may be partially explained by the extent of heterogeneity observed by REA. These findings support the contention that the MAIS complex has a substantially greater degree of heterogeneity than has been revealed by traditional methods.

Full text

PDF

Images in this article

2311Image
on p.2311
2311Image
on p.2311
2311Image
on p.2311

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baess I., Magnusson M. Classification of Mycobacterium simiae by means of comparative reciprocal intradermal sensitin testing on guinea-pigs and deoxyribonucleic acid hybridization. Acta Pathol Microbiol Immunol Scand B. 1982 Apr;90(2):101–107. doi: 10.1111/j.1699-0463.1982.tb00089.x. [DOI] [PubMed] [Google Scholar]
  2. Berlin O. G., Zakowski P., Bruckner D. A., Clancy M. N., Johnson B. L., Jr Mycobacterium avium: a pathogen of patients with acquired immunodeficiency syndrome. Diagn Microbiol Infect Dis. 1984 Jun;2(3):213–218. doi: 10.1016/0732-8893(84)90033-6. [DOI] [PubMed] [Google Scholar]
  3. Bradbury W. C., Murray R. G., Mancini C., Morris V. L. Bacterial chromosomal restriction endonuclease analysis of the homology of Bacteroides species. J Clin Microbiol. 1985 Jan;21(1):24–28. doi: 10.1128/jcm.21.1.24-28.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Collins D. M., De Lisle G. W. DNA restriction endonuclease analysis of Mycobacterium bovis and other members of the tuberculosis complex. J Clin Microbiol. 1985 Apr;21(4):562–564. doi: 10.1128/jcm.21.4.562-564.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Collins D. M., De Lisle G. W. DNA restriction endonuclease analysis of Mycobacterium tuberculosis and Mycobacterium bovis BCG. J Gen Microbiol. 1984 Apr;130(4):1019–1021. doi: 10.1099/00221287-130-4-1019. [DOI] [PubMed] [Google Scholar]
  6. Collins D. M., de Lisle G. W. Restriction endonuclease analysis of various strains of Mycobacterium paratuberculosis isolated from cattle. Am J Vet Res. 1986 Oct;47(10):2226–2229. [PubMed] [Google Scholar]
  7. Crawford J. T., Bates J. H. Phage typing of the Mycobacterium avium-intracellulare-scrofulaceum complex. A study of strains of diverse geographic and host origin. Am Rev Respir Dis. 1985 Aug;132(2):386–389. doi: 10.1164/arrd.1985.132.2.386. [DOI] [PubMed] [Google Scholar]
  8. Crawford J. T., Cave M. D., Bates J. H. Characterization of plasmids from strains of Mycobacterium avium-intracellulare. Rev Infect Dis. 1981 Sep-Oct;3(5):949–952. doi: 10.1093/clinids/3.5.949. [DOI] [PubMed] [Google Scholar]
  9. Hawkins C. C., Gold J. W., Whimbey E., Kiehn T. E., Brannon P., Cammarata R., Brown A. E., Armstrong D. Mycobacterium avium complex infections in patients with the acquired immunodeficiency syndrome. Ann Intern Med. 1986 Aug;105(2):184–188. doi: 10.7326/0003-4819-105-2-184. [DOI] [PubMed] [Google Scholar]
  10. Jenkins P. A., Marks J., Schaefer W. B. Thin-layer chromatography of mycobacterial lipids as an aid to classification: the scotochromogenic mycobacteria, including Mycobacterium scrofulaceum, M. xenopi, M. aquae, M. gordonae, M. flavescens. Tubercle. 1972 Jun;53(2):118–127. doi: 10.1016/0041-3879(72)90028-1. [DOI] [PubMed] [Google Scholar]
  11. Kiehn T. E., Edwards F. F., Brannon P., Tsang A. Y., Maio M., Gold J. W., Whimbey E., Wong B., McClatchy J. K., Armstrong D. Infections caused by Mycobacterium avium complex in immunocompromised patients: diagnosis by blood culture and fecal examination, antimicrobial susceptibility tests, and morphological and seroagglutination characteristics. J Clin Microbiol. 1985 Feb;21(2):168–173. doi: 10.1128/jcm.21.2.168-173.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kubica G. P., Silcox V. A. Numberical taxonomic analysis of some slowly growing mycobacteria using hypothetical median strain patterns. J Gen Microbiol. 1973 Jan;74(1):149–158. doi: 10.1099/00221287-74-1-149. [DOI] [PubMed] [Google Scholar]
  13. Le Pecq J. B., Paoletti C. A new fluorometric method for RNA and DNA determination. Anal Biochem. 1966 Oct;17(1):100–107. doi: 10.1016/0003-2697(66)90012-1. [DOI] [PubMed] [Google Scholar]
  14. Mankiewicz E. Agents of mycobacterial variations. Rev Infect Dis. 1981 Sep-Oct;3(5):926–933. doi: 10.1093/clinids/3.5.926. [DOI] [PubMed] [Google Scholar]
  15. Marshall R. B., Wilton B. E., Robinson A. J. Identification of Leptospira serovars by restriction-endonuclease analysis. J Med Microbiol. 1981 Feb;14(1):163–166. doi: 10.1099/00222615-14-1-163. [DOI] [PubMed] [Google Scholar]
  16. Matthews P. R., McDiarmid A., Collins P., Brown A. The dependence of some strains of Mycobacterium avium on mycobactin for initial and subsequent growth. J Med Microbiol. 1978 Feb;11(1):53–57. doi: 10.1099/00222615-11-1-53. [DOI] [PubMed] [Google Scholar]
  17. Meissner G., Anz W. Sources of Mycobacterium avium complex infection resulting in human diseases. Am Rev Respir Dis. 1977 Dec;116(6):1057–1064. doi: 10.1164/arrd.1977.116.6.1057. [DOI] [PubMed] [Google Scholar]
  18. Meissner G., Schröder K. H., Amadio G. E., Anz W., Chaparas S., Engel H. W., Jenkins P. A., Käppler W., Kleeberg H. H., Kubala E. A co-operative numerical analysis of nonscoto- and nonphotochromogenic slowly growing mycobacteria. J Gen Microbiol. 1974 Aug;83(2):207–235. doi: 10.1099/00221287-83-2-207. [DOI] [PubMed] [Google Scholar]
  19. Meissner P. S., Falkinham J. O., 3rd Plasmid DNA profiles as epidemiological markers for clinical and environmental isolates of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum. J Infect Dis. 1986 Feb;153(2):325–331. doi: 10.1093/infdis/153.2.325. [DOI] [PubMed] [Google Scholar]
  20. Patel R., Kvach J. T., Mounts P. Isolation and restriction endonuclease analysis of mycobacterial DNA. J Gen Microbiol. 1986 Feb;132(2):541–551. doi: 10.1099/00221287-132-2-541. [DOI] [PubMed] [Google Scholar]
  21. Portaels F. Difficulties encountered in identification of M. avium-M. intracellulare, M. scrofulaceum, and related strains. Am Rev Respir Dis. 1978 Nov;118(5):969–969. doi: 10.1164/arrd.1978.118.5.969. [DOI] [PubMed] [Google Scholar]
  22. Schaefer W. B. Serologic identification and classification of the atypical mycobacteria by their agglutination. Am Rev Respir Dis. 1965 Dec;92(6):85–93. doi: 10.1164/arrd.1965.92.6P2.85. [DOI] [PubMed] [Google Scholar]
  23. Shoemaker S. A., Fisher J. H., Jones W. D., Jr, Scoggin C. H. Restriction fragment analysis of chromosomal DNA defines different strains of Mycobacterium tuberculosis. Am Rev Respir Dis. 1986 Aug;134(2):210–213. doi: 10.1164/arrd.1986.134.2.210. [DOI] [PubMed] [Google Scholar]
  24. Stanford J. L. Immunodiffusion analysis--a rational basis for the taxonomy of mycobacteria. Ann Soc Belg Med Trop. 1973;53(4):321–330. [PubMed] [Google Scholar]
  25. Timme T. L., Brennan P. J. Induction of bacteriophage from members of the Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium scrofulaceum serocomplex. J Gen Microbiol. 1984 Aug;130(8):2059–2066. doi: 10.1099/00221287-130-8-2059. [DOI] [PubMed] [Google Scholar]
  26. Wolinsky E. Nontuberculous mycobacteria and associated diseases. Am Rev Respir Dis. 1979 Jan;119(1):107–159. doi: 10.1164/arrd.1979.119.1.107. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES