Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1987 Dec;25(12):2339–2343. doi: 10.1128/jcm.25.12.2339-2343.1987

Serum antibody response to capsular polysaccharide, outer membrane, and lipooligosaccharide in children with invasive Haemophilus influenzae type b infections.

B A Claesson 1, T Lagergård 1, B Trollfors 1, L Gothefors 1, U Jodal 1
PMCID: PMC269484  PMID: 3501433

Abstract

Serum antibodies against capsular polysaccharide (CPS), outer membrane (OM), and lipooligosaccharide (LOS) from Haemophilus influenzae type b were measured by enzyme-linked immunosorbent assay in acute- and convalescent-phase sera from 21 children between 3 months and 4 years of age with invasive H. influenzae type b infections. As expected, the levels of anti-CPS antibodies in the acute-phase serum samples were low or not detectable, as were the levels of antibodies against LOS. In contrast, all children had detectable antibodies against the OM in the acute-phase serum sample, indicating that they are of little or no importance for protection. An antibody response to CPS was noted in 13 of the 21 patients, mainly in the older children. An antibody response to the OM was seen in 16 patients, with no evident relation to age. The antibody response to the OM preparation, which consisted of proteins and LOS, was probably directed mainly against the OM proteins, since only six children showed a response, usually of low magnitude, of antibodies to LOS.

Full text

PDF
2339

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dahlberg-Lagergård T. Bactericidal antibodies against noncapsular components of Haemophilus influenzae. J Clin Microbiol. 1983 Mar;17(3):428–431. doi: 10.1128/jcm.17.3.428-431.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dahlberg-Lagergård T. Target antigens for bactericidal and opsonizing antibodies to Haemophilus influenzae. Acta Pathol Microbiol Immunol Scand C. 1982 Aug;90(4):209–216. doi: 10.1111/j.1699-0463.1982.tb01440.x. [DOI] [PubMed] [Google Scholar]
  3. Edmonson M. B., Granoff D. M., Barenkamp S. J., Chesney P. J. Outer membrane protein subtypes and investigation of recurrent Haemophilus influenzae type b disease. J Pediatr. 1982 Feb;100(2):202–208. doi: 10.1016/s0022-3476(82)80635-5. [DOI] [PubMed] [Google Scholar]
  4. Gulig P. A., McCracken G. H., Jr, Frisch C. F., Johnston K. H., Hansen E. J. Antibody response of infants to cell surface-exposed outer membrane proteins of Haemophilus influenzae type b after systemic Haemophilus disease. Infect Immun. 1982 Jul;37(1):82–88. doi: 10.1128/iai.37.1.82-88.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hansen E. J., Robertson S. M., Gulig P. A., Frisch C. F., Haanes E. J. Immunoprotection of rats against Haemophilus influenzae type B disease mediated by monoclonal antibody against a haemophilus outer-membrane protein. Lancet. 1982 Feb 13;1(8268):366–368. doi: 10.1016/s0140-6736(82)91394-0. [DOI] [PubMed] [Google Scholar]
  6. Kaplan S. L., Mason E. O., Jr, Johnson G., Broughton R. A., Hurley D., Parke J. C., Jr Enzyme-linked immunosorbent assay for detection of capsular antibodies against Haemophilus influenzae type b: comparison with radioimmunoassay. J Clin Microbiol. 1983 Nov;18(5):1201–1204. doi: 10.1128/jcm.18.5.1201-1204.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kimura A., Gulig P. A., McCracken G. H., Jr, Loftus T. A., Hansen E. J. A minor high-molecular-weight outer membrane protein of Haemophilus influenzae type b is a protective antigen. Infect Immun. 1985 Jan;47(1):253–259. doi: 10.1128/iai.47.1.253-259.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Käyhty H., Jousimies-Somer H., Peltola H., Mäketä P. H. Antibody response to capsular polysaccharides of groups A and C neisseria meningitidis and Haemophilus influenzae type b during bacteremic disease. J Infect Dis. 1981 Jan;143(1):32–41. doi: 10.1093/infdis/143.1.32. [DOI] [PubMed] [Google Scholar]
  9. Käyhty H., Peltola H., Karanko V., Mäkelä P. H. The protective level of serum antibodies to the capsular polysaccharide of Haemophilus influenzae type b. J Infect Dis. 1983 Jun;147(6):1100–1100. doi: 10.1093/infdis/147.6.1100. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Lagergård T., Nylén O., Sandberg T., Trollfors B. Antibody responses to capsular polysaccharide, lipopolysaccharide, and outer membrane in adults infected with Haemophilus influenzae type b. J Clin Microbiol. 1984 Dec;20(6):1154–1158. doi: 10.1128/jcm.20.6.1154-1158.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Loeb M. R., Smith D. H. Human antibody response to individual outer membrane proteins of Haemophilus influenzae type b. Infect Immun. 1982 Sep;37(3):1032–1036. doi: 10.1128/iai.37.3.1032-1036.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Murphy T. F., Nelson M. B., Dudas K. C., Mylotte J. M., Apicella M. A. Identification of a specific epitope of Haemophilus influenzae on a 16,600-dalton outer membrane protein. J Infect Dis. 1985 Dec;152(6):1300–1307. doi: 10.1093/infdis/152.6.1300. [DOI] [PubMed] [Google Scholar]
  14. Musher D., Goree A., Murphy T., Chapman A., Zahradnik J., Apicella M., Baughn R. Immunity to Haemophilus influenzae type b in young adults: correlation of bactericidal and opsonizing activity of serum with antibody to polyribosylribitol phosphate and lipooligosaccharide before and after vaccination. J Infect Dis. 1986 Dec;154(6):935–943. doi: 10.1093/infdis/154.6.935. [DOI] [PubMed] [Google Scholar]
  15. Myerowitz R. L., Norden C. W., Demchak T. A. Significance of noncapsular antigens in protection against experimental Haemophilus influenzae type b disease: cross-reactivity. Infect Immun. 1978 Aug;21(2):619–626. doi: 10.1128/iai.21.2.619-626.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Norden C. W., Michaels R. H., Melish M. Serologic responses of children with meningitis due to Haemophilus influenzae type b. J Infect Dis. 1976 Nov;134(5):495–499. doi: 10.1093/infdis/134.5.495. [DOI] [PubMed] [Google Scholar]
  17. Norden C. W., Michaels R. Immunologic response in patients with epiglottitis caused by Haemophilus influenzae type b. J Infect Dis. 1973 Dec;128(6):777–780. doi: 10.1093/infdis/128.6.777. [DOI] [PubMed] [Google Scholar]
  18. Peltola H., Käyhty H., Sivonen A., Mäkelä H. Haemophilus influenzae type b capsular polysaccharide vaccine in children: a double-blind field study of 100,000 vaccinees 3 months to 5 years of age in Finland. Pediatrics. 1977 Nov;60(5):730–737. [PubMed] [Google Scholar]
  19. Ramadas K., Petersen G. M., Heiner D. C., Ward J. I. Class and subclass antibodies to Haemophilus influenzae type b capsule: comparison of invasive disease and natural exposure. Infect Immun. 1986 Sep;53(3):486–490. doi: 10.1128/iai.53.3.486-490.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Robbins J. B., Parke J. C., Jr, Schneerson R., Whisnant J. K. Quantitative measurement of "natural" and immunization-induced Haemophilus influenzae type b capsular polysaccharide antibodies. Pediatr Res. 1973 Mar;7(3):103–110. doi: 10.1203/00006450-197303000-00001. [DOI] [PubMed] [Google Scholar]
  21. Rosales S. V., Lascolea L. J., Jr, Ogra P. L. Development of respiratory mucosal tolerance during Haemophilus influenzae type B infection in infancy. J Immunol. 1984 Mar;132(3):1517–1521. [PubMed] [Google Scholar]
  22. Schneerson R., Robbins J. B., Parke J. C., Jr, Bell C., Schlesselman J. J., Sutton A., Wang Z., Schiffman G., Karpas A., Shiloach J. Quantitative and qualitative analyses of serum antibodies elicited in adults by Haemophilus influenzae type b and pneumococcus type 6A capsular polysaccharide-tetanus toxoid conjugates. Infect Immun. 1986 May;52(2):519–528. doi: 10.1128/iai.52.2.519-528.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shenep J. L., Munson R. S., Jr, Barenkamp S. J., Granoff D. M. Further studies of the role of noncapsular antibody in protection against experimental Haemophilus influenzae type b bacteremia. Infect Immun. 1983 Oct;42(1):257–263. doi: 10.1128/iai.42.1.257-263.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shenep J. L., Munson R. S., Jr, Granoff D. M. Human antibody responses to lipopolysaccharide after meningitis due to Haemophilus influenzae type b. J Infect Dis. 1982 Feb;145(2):181–190. doi: 10.1093/infdis/145.2.181. [DOI] [PubMed] [Google Scholar]
  25. Smith A. L., Smith D. H., Averill D. R., Jr, Marino J., Moxon E. R. Production of Haemophilus influenzae b meningitis in infant rats by intraperitoneal inoculation. Infect Immun. 1973 Aug;8(2):278–290. doi: 10.1128/iai.8.2.278-290.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ward J. I., Lum M. K., Hall D. B., Silimperi D. R., Bender T. R. Invasive Haemophilus influenzae type b disease in Alaska: background epidemiology for a vaccine efficacy trial. J Infect Dis. 1986 Jan;153(1):17–26. doi: 10.1093/infdis/153.1.17. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES