Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1987 Dec;25(12):2384–2387. doi: 10.1128/jcm.25.12.2384-2387.1987

Molecular epidemiology of rotavirus in black infants in South Africa.

A D Steele 1, J J Alexander 1
PMCID: PMC269494  PMID: 2828416

Abstract

The molecular epidemiology of rotavirus infections in black infants in Ga-Rankuwa, South Africa, was investigated by polyacrylamide gel electrophoresis. Between 1983 and 1986, 14 different RNA electrophoretic patterns were observed for children with acute gastroenteritis. These electrophoretypes showed a sequential pattern of appearance, with a limited number being present at any one time. In contrast, for neonates only one RNA electrophoretype was detected, which persisted for at least 3 years.

Full text

PDF
2384

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beards G. M. Polymorphism of genomic RNAs within rotavirus serotypes and subgroups. Arch Virol. 1982;74(1):65–70. doi: 10.1007/BF01320783. [DOI] [PubMed] [Google Scholar]
  2. Besselaar T. G., Rosenblatt A., Kidd A. H. Atypical rotavirus from South African neonates. Brief report. Arch Virol. 1986;87(3-4):327–330. doi: 10.1007/BF01315311. [DOI] [PubMed] [Google Scholar]
  3. Bishop R. F., Barnes G. L., Cipriani E., Lund J. S. Clinical immunity after neonatal rotavirus infection. A prospective longitudinal study in young children. N Engl J Med. 1983 Jul 14;309(2):72–76. doi: 10.1056/NEJM198307143090203. [DOI] [PubMed] [Google Scholar]
  4. Bridger J. C., Pedley S., McCrae M. A. Group C rotaviruses in humans. J Clin Microbiol. 1986 Apr;23(4):760–763. doi: 10.1128/jcm.23.4.760-763.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Buitenwerf J., Nuilwijk-van Alphen M., Schaap G. J. Characterization of rotaviral RNA isolated from children with gastroenteritis in two hospitals in Rotterdam. J Med Virol. 1983;12(1):71–78. doi: 10.1002/jmv.1890120108. [DOI] [PubMed] [Google Scholar]
  6. Chrystie I. L., Totterdell B. M., Banatvala J. E. Asymptomatic endemic rotavirus infections in the newborn. Lancet. 1978 Jun 3;1(8075):1176–1178. doi: 10.1016/S0140-6736(78)90967-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Clarke I. N., McCrae M. A. A sensitive method for the production of diagnostic fingerprints of the genome segments of field isolates of rotavirus. J Virol Methods. 1981 Dec;3(5):261–269. doi: 10.1016/0166-0934(81)90063-x. [DOI] [PubMed] [Google Scholar]
  8. Dimitrov D. H., Estes M. K., Rangelova S. M., Shindarov L. M., Melnick J. L., Graham D. Y. Detection of antigenically distinct rotaviruses from infants. Infect Immun. 1983 Aug;41(2):523–526. doi: 10.1128/iai.41.2.523-526.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Espejo R. T., Muñz O., Serafin F., Romero P. Shift in the prevalent human rotavirus detected by ribonucleic acid segment differences. Infect Immun. 1980 Feb;27(2):351–354. doi: 10.1128/iai.27.2.351-354.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Estes M. K., Graham D. Y., Dimitrov D. H. The molecular epidemiology of rotavirus gastroenteritis. Prog Med Virol. 1984;29:1–22. [PubMed] [Google Scholar]
  11. Flores J., Midthun K., Hoshino Y., Green K., Gorziglia M., Kapikian A. Z., Chanock R. M. Conservation of the fourth gene among rotaviruses recovered from asymptomatic newborn infants and its possible role in attenuation. J Virol. 1986 Dec;60(3):972–979. doi: 10.1128/jvi.60.3.972-979.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Follett E. A., Sanders R. C., Beards G. M., Hundley F., Desselberger U. Molecular epidemiology of human rotaviruses. Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83. J Hyg (Lond) 1984 Apr;92(2):209–222. doi: 10.1017/s0022172400064238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Forster J., Pastor S. Epidemiology of human rotaviruses as determined by electrophoresis of genome RNA. Eur J Clin Microbiol. 1983 Apr;2(2):141–147. doi: 10.1007/BF02001580. [DOI] [PubMed] [Google Scholar]
  14. Herring A. J., Inglis N. F., Ojeh C. K., Snodgrass D. R., Menzies J. D. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol. 1982 Sep;16(3):473–477. doi: 10.1128/jcm.16.3.473-477.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Murphy A. M., Albrey M. B., Crewe E. B. Rotavirus infections of neonates. Lancet. 1977 Dec 3;2(8049):1149–1150. doi: 10.1016/S0140-6736(77)91538-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nicolas J. C., Cohen J., Fortier B., Lourenco M. H., Bricout F. Isolation of a human pararotavirus. Virology. 1983 Jan 15;124(1):181–184. doi: 10.1016/0042-6822(83)90302-1. [DOI] [PubMed] [Google Scholar]
  18. Offit P. A., Blavat G., Greenberg H. B., Clark H. F. Molecular basis of rotavirus virulence: role of gene segment 4. J Virol. 1986 Jan;57(1):46–49. doi: 10.1128/jvi.57.1.46-49.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pedley S., Bridger J. C., Brown J. F., McCrae M. A. Molecular characterization of rotaviruses with distinct group antigens. J Gen Virol. 1983 Oct;64(Pt 10):2093–2101. doi: 10.1099/0022-1317-64-10-2093. [DOI] [PubMed] [Google Scholar]
  20. Perez-Schael I., Daoud G., White L., Urbina G., Daoud N., Perez M., Flores J. Rotavirus shedding by newborn children. J Med Virol. 1984;14(2):127–136. doi: 10.1002/jmv.1890140206. [DOI] [PubMed] [Google Scholar]
  21. Rodger S. M., Bishop R. F., Birch C., McLean B., Holmes I. H. Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid. J Clin Microbiol. 1981 Feb;13(2):272–278. doi: 10.1128/jcm.13.2.272-278.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schnagl R. D., Rodger S. M., Holmes I. H. Variation in human rotavirus electropherotypes occurring between rotavirus gastroenteritis epidemics in central Australia. Infect Immun. 1981 Jul;33(1):17–21. doi: 10.1128/iai.33.1.17-21.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Soenarto Y., Sebodo T., Ridho R., Alrasjid H., Rohde J. E., Bugg H. C., Barnes G. L., Bishop R. F. Acute diarrhea and rotavirus infection in newborn babies and children in Yogyakarta, Indonesia, from June 1978 to June 1979. J Clin Microbiol. 1981 Aug;14(2):123–129. doi: 10.1128/jcm.14.2.123-129.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Spencer E., Avendaño F., Araya M. Characteristics and analysis of electropherotypes of human rotavirus isolated in Chile. J Infect Dis. 1983 Jul;148(1):41–48. doi: 10.1093/infdis/148.1.41. [DOI] [PubMed] [Google Scholar]
  25. Steele A. D., Alexander J. J., Hay I. T. Rotavirus-associated gastroenteritis in black infants in South Africa. J Clin Microbiol. 1986 May;23(5):992–994. doi: 10.1128/jcm.23.5.992-994.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Svensson L., Uhnoo I., Grandien M., Wadell G. Molecular epidemiology of rotavirus infections in Uppsala, Sweden, 1981: disappearance of a predominant electropherotype. J Med Virol. 1986 Feb;18(2):101–111. doi: 10.1002/jmv.1890180202. [DOI] [PubMed] [Google Scholar]
  27. Tam J. S., Kum W. W., Lam B., Yeung C. Y., Ng M. H. Molecular epidemiology of human rotavirus infection in children in Hong Kong. J Clin Microbiol. 1986 Mar;23(3):660–664. doi: 10.1128/jcm.23.3.660-664.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Uhnoo I., Svensson L. Clinical and epidemiological features of acute infantile gastroenteritis associated with human rotavirus subgroups 1 and 2. J Clin Microbiol. 1986 Mar;23(3):551–555. doi: 10.1128/jcm.23.3.551-555.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES