Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1987 Dec;25(12):2415–2417. doi: 10.1128/jcm.25.12.2415-2417.1987

Antibody that inhibits human immunodeficiency virus reverse transcriptase and association with inability to isolate virus.

K Sano 1, M H Lee 1, F Morales 1, P Nishanian 1, J Fahey 1, R Detels 1, D T Imagawa 1
PMCID: PMC269505  PMID: 2448336

Abstract

Most individuals infected with human immunodeficiency virus (HIV), the causative agent of acquired immune deficiency syndrome, produce an antibody against the viral reverse transcriptase (RT). Our studies show that 67% of HIV-seropositive individuals (33 of 49) produced an antibody that specifically inhibited viral RT enzyme activity. We were able to isolate HIV from only 18% of these individuals (6 of 33). On the other hand, virus was readily isolated from 63% of HIV-seropositive individuals (10 of 16) who did not demonstrate this antibody. Further examination of this RT-inhibiting antibody and its role during virus infection is needed, as it may prove to be of diagnostic, prognostic, or therapeutic value in this study and treatment of acquired immune deficiency syndrome.

Full text

PDF
2415

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barré-Sinoussi F., Chermann J. C., Rey F., Nugeyre M. T., Chamaret S., Gruest J., Dauguet C., Axler-Blin C., Vézinet-Brun F., Rouzioux C. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science. 1983 May 20;220(4599):868–871. doi: 10.1126/science.6189183. [DOI] [PubMed] [Google Scholar]
  2. Gallo R. C., Salahuddin S. Z., Popovic M., Shearer G. M., Kaplan M., Haynes B. F., Palker T. J., Redfield R., Oleske J., Safai B. Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. Science. 1984 May 4;224(4648):500–503. doi: 10.1126/science.6200936. [DOI] [PubMed] [Google Scholar]
  3. Harada S., Koyanagi Y., Yamamoto N. Infection of human T-lymphotropic virus type-I (HTLV-I)-bearing MT-4 cells with HTLV-III (AIDS virus): chronological studies of early events. Virology. 1985 Oct 30;146(2):272–281. doi: 10.1016/0042-6822(85)90010-8. [DOI] [PubMed] [Google Scholar]
  4. Hollis V. W., Jr, Aoki T., Barrera O., Oldstone M. B., Dixon F. J. Detection of naturally occurring antibodies to RNA-dependent DNA polymerase of murine leukemia virus in kidney eluates of AKR mice. J Virol. 1974 Feb;13(2):448–454. doi: 10.1128/jvi.13.2.448-454.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jacquemin P. C., Saxinger C., Gallo R. C., Hardy W. D., Jr, Essex M. Antibody response in cats to feline leukemia virus reverse transcriptase under natural conditions of exposure to the virus. Virology. 1978 Dec;91(2):472–476. doi: 10.1016/0042-6822(78)90393-8. [DOI] [PubMed] [Google Scholar]
  6. Laurence J., Saunders A., Kulkosky J. Characterization and clinical association of antibody inhibitory to HIV reverse transcriptase activity. Science. 1987 Mar 20;235(4795):1501–1504. doi: 10.1126/science.2435004. [DOI] [PubMed] [Google Scholar]
  7. Levy J. A., Hoffman A. D., Kramer S. M., Landis J. A., Shimabukuro J. M., Oshiro L. S. Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS. Science. 1984 Aug 24;225(4664):840–842. doi: 10.1126/science.6206563. [DOI] [PubMed] [Google Scholar]
  8. Mullins J. I., Chen C. S., Hoover E. A. Disease-specific and tissue-specific production of unintegrated feline leukaemia virus variant DNA in feline AIDS. Nature. 1986 Jan 23;319(6051):333–336. doi: 10.1038/319333a0. [DOI] [PubMed] [Google Scholar]
  9. Nishanian P., Taylor J. M., Korns E., Detels R., Saah A., Fahey J. L. Significance of quantitative enzyme-linked immunosorbent assay (ELISA) results in evaluation of three ELISAs and Western blot tests for detection of antibodies to human immunodeficiency virus in a high-risk population. J Clin Microbiol. 1987 Feb;25(2):395–400. doi: 10.1128/jcm.25.2.395-400.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rho H. M., Poiesz B., Ruscetti F. W., Gallo R. C. Characterization of the reverse transcriptase from a new retrovirus (HTLV) produced by a human cutaneous T-cell lymphoma cell line. Virology. 1981 Jul 15;112(1):355–360. doi: 10.1016/0042-6822(81)90642-5. [DOI] [PubMed] [Google Scholar]
  11. Robert-Gurof M., Gallo R. C. Type-specific binding antibody to baboon endogenous virus (M7) reverse transcriptase. J Gen Virol. 1979 Apr;43(1):241–246. doi: 10.1099/0022-1317-43-1-241. [DOI] [PubMed] [Google Scholar]
  12. Sherr C. J., Fedele L. A., Benveniste R. E., Todaro G. J. Interspecies antigenic determinants of the reverse transcriptases and p30 proteins of mammalian type C viruses. J Virol. 1975 Jun;15(6):1440–1448. doi: 10.1128/jvi.15.6.1440-1448.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Temin H. M., Baltimore D. RNA-directed DNA synthesis and RNA tumor viruses. Adv Virus Res. 1972;17:129–186. doi: 10.1016/s0065-3527(08)60749-6. [DOI] [PubMed] [Google Scholar]
  14. Verma I. M. The reverse transcriptase. Biochim Biophys Acta. 1977 Mar 21;473(1):1–38. doi: 10.1016/0304-419x(77)90005-1. [DOI] [PubMed] [Google Scholar]
  15. Wuu K. D., Graves D. C., Ferrer J. F. Inhibition of the reverse transcriptase of bovine leukemia virus by antibody in sera from leukemic cattle and immunological characterization of the enzyme. Cancer Res. 1977 May;37(5):1438–1442. [PubMed] [Google Scholar]
  16. di Marzo Veronese F., Copeland T. D., DeVico A. L., Rahman R., Oroszlan S., Gallo R. C., Sarngadharan M. G. Characterization of highly immunogenic p66/p51 as the reverse transcriptase of HTLV-III/LAV. Science. 1986 Mar 14;231(4743):1289–1291. doi: 10.1126/science.2418504. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES