Abstract
The initial evaluation of a fluorescence, nonmicroscopic method of rapid identification of Candida albicans is described. A total of 524 yeast isolates were evaluated in parallel by the umbelliferyl-conjugated N-acetyl-beta-D-galactosaminide (UAG) test and the germ tube (GT) test in comparison with the API 20C Yeast Identification System. The UAG test correctly identified 333 of the 334 isolates of C. albicans (99.7%), and the GT test identified 328 (98%). There were three false-positive GT reactions and five false-positive UAG reactions with 69 isolates of C. tropicalis. The sensitivity and specificity were 99 and 97%, respectively, for the UAG test and were both 98% for the GT test. The UAG test requires less than 2 h for test completion compared with 2 to 4 h for the GT test, is similar in cost, has a nonmicroscopic visual endpoint, and eliminates the health hazards of handling pooled human sera.
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Selected References
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- Berardinelli S., Opheim D. J. New germ tube induction medium for the identification of Candida albicans. J Clin Microbiol. 1985 Nov;22(5):861–862. doi: 10.1128/jcm.22.5.861-862.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bobey D. G., Ederer G. M. Rapid detection of yeast enzymes by using 4-methylumbelliferyl substrates. J Clin Microbiol. 1981 Feb;13(2):393–394. doi: 10.1128/jcm.13.2.393-394.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fell J. W., Meyer S. A. Systematics of yeast species in the Candida parapsilosis group. Mycopathol Mycol Appl. 1967 Aug 14;32(3):177–193. doi: 10.1007/BF02049795. [DOI] [PubMed] [Google Scholar]
- Huppert M., Harper G., Sun S. H., Delanerolle V. Rapid methods for identification of yeasts. J Clin Microbiol. 1975 Jul;2(1):21–34. doi: 10.1128/jcm.2.1.21-34.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Katsura Y., Uesaka I. Assessment of germ tube dispersion activity of serum from experimental candidiasis: a new procedure for serodiagnosis. Infect Immun. 1974 May;9(5):788–793. doi: 10.1128/iai.9.5.788-793.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LANDAU J. W., DABROWA N., NEWCOMER V. D. THE RAPID FORMATION IN SERUM OF FILAMENTS BY CANDIDA ALBICANS. J Invest Dermatol. 1965 Mar;44:171–179. [PubMed] [Google Scholar]
- Mackenzie D. W. Serum tube identification of Candida albicans. J Clin Pathol. 1962 Nov;15(6):563–565. doi: 10.1136/jcp.15.6.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maddocks J. L., Greenan M. J. A rapid method for identifying bacterial enzymes. J Clin Pathol. 1975 Aug;28(8):686–687. doi: 10.1136/jcp.28.8.686. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martin M. V. Germ-tube formation by oral strains of Candida tropicalis. J Med Microbiol. 1979 May;12(2):187–193. doi: 10.1099/00222615-12-2-187. [DOI] [PubMed] [Google Scholar]
- Martin M. V., White F. H. A microbiologic and ultrastructural investigation of germ-tube formation by oral strains of Candida tropicalis. Am J Clin Pathol. 1981 May;75(5):671–676. doi: 10.1093/ajcp/75.5.671. [DOI] [PubMed] [Google Scholar]
- Salkin I. F., Land G. A., Hurd N. J., Goldson P. R., McGinnis M. R. Evaluation of YeastIdent and Uni-Yeast-Tek yeast identification systems. J Clin Microbiol. 1987 Apr;25(4):624–627. doi: 10.1128/jcm.25.4.624-627.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taschdjian C. L., Kozinn P. J., Toni E. F. Opportunistic yeast infections, with special reference to candidiasis. Ann N Y Acad Sci. 1970 Oct 30;174(2):606–622. doi: 10.1111/j.1749-6632.1970.tb45586.x. [DOI] [PubMed] [Google Scholar]