Figure 1.
Mdm2 interacts with the general transcription factor TFIID in cultured cells. SJSA cells (6 × 107) were harvested and proteins in the extracts were immunoprecipitated with anti-TAFII250 monoclonal antibody (clone 6B3), anti-Mdm2 monoclonal antibodies (SMP14 and 4B2) and IgG mouse as a control. Immunoprecipitates were analysed by Western blotting with anti-TAFII250 antibody or anti-Mdm2 antibodies as indicated.