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. 1990 Jan;28(1):59–64. doi: 10.1128/jcm.28.1.59-64.1990

Evaluation of an enzyme-linked immunosorbent assay that uses ferrous metal beads for determination of antihistoplasmal immunoglobulins G and M.

S E Zimmerman 1, M L French 1, M B Kleiman 1, L J Wheat 1
PMCID: PMC269537  PMID: 2105336

Abstract

A rapid enzyme-linked immunosorbent assay (ELISA) for the detection of human class-specific antibodies to Histoplasma capsulatum (histoplasmal immunoglobulin M [HIgM] and histoplasmal IgG [HIgG]) was developed by using antigen adsorbed onto polycarbonate-coated ferrous beads. In the ELISA method all the reagents used were commercially available. In 135 specimens from patients with confirmed histoplasmosis, sensitivities were 76% for complement fixation (CF), 53% for immunodiffusion (ID), and 64% for the ELISA for HIgM and HIgG combined. The ELISA detected histoplasmal antibody in 36% of the specimens with negative antibody titers by CF and 46% of the specimens with negative antibody titers by ID. The ELISA detected histoplasmal antibody in 27% of specimens that were negative by both CF and ID. When limited to specimens collected within 4 months of the onset of histoplasmosis symptoms, sensitivities were 82% for CF, 63% for ID, and 86% for ELISA for HIgG and HIgM combined. Within this group, ELISA detected histoplasmal antibody in 90% of the specimens that were negative by CF, 76% that were negative by ID, and 100% that were negative by both CF and ID. The specificity of the ELISA could not be fully addressed since sera from patients with other fungal infections were not available.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauman D. S., Smith C. D. Comparison of immunodiffusion and complement fixation tests in the diagnosis of histoplasmosis. J Clin Microbiol. 1976 Aug;2(2):77–80. [PMC free article] [PubMed] [Google Scholar]
  2. Goodwin R. A., Loyd J. E., Des Prez R. M. Histoplasmosis in normal hosts. Medicine (Baltimore) 1981 Jul;60(4):231–266. doi: 10.1097/00005792-198107000-00001. [DOI] [PubMed] [Google Scholar]
  3. Reiss E., Hutchinson H., Pine L., Ziegler D. W., Kaufman L. Solid-phase competitive-binding radioimmunoassay for detecting antibody to the M antigen of histoplasmin. J Clin Microbiol. 1977 Dec;6(6):598–604. doi: 10.1128/jcm.6.6.598-604.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Sathapatayavongs B., Batteiger B. E., Wheat J., Slama T. G., Wass J. L. Clinical and laboratory features of disseminated histoplasmosis during two large urban outbreaks. Medicine (Baltimore) 1983 Sep;62(5):263–270. doi: 10.1097/00005792-198309000-00001. [DOI] [PubMed] [Google Scholar]
  5. Sprouse R. F., Caldwell C. W., Everett E. D. Radioimmunoassays that use 125I-labeled protein A for determination of Histoplasma capsulatum-specific immunoglobulin G, A, and M class antibodies in histoplasmosis. J Clin Microbiol. 1981 Jan;13(1):66–72. doi: 10.1128/jcm.13.1.66-72.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Terry P. B., Rosenow E. C., 3rd, Roberts G. D. False-positive complement-fixation serology in histoplasmosis. A retrospective study. JAMA. 1978 Jun 9;239(23):2453–2456. doi: 10.1001/jama.239.23.2453. [DOI] [PubMed] [Google Scholar]
  7. Wheat J., French M. L., Kamel S., Tewari R. P. Evaluation of cross-reactions in Histoplasma capsulatum serologic tests. J Clin Microbiol. 1986 Mar;23(3):493–499. doi: 10.1128/jcm.23.3.493-499.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Wheat J., French M. L., Kohler R. B., Zimmerman S. E., Smith W. R., Norton J. A., Eitzen H. E., Smith C. D., Slama T. G. The diagnostic laboratory tests for histoplasmosis: analysis of experience in a large urban outbreak. Ann Intern Med. 1982 Nov;97(5):680–685. doi: 10.7326/0003-4819-97-5-680. [DOI] [PubMed] [Google Scholar]
  9. Wheat L. J., Kohler R. B., French M. L., Garten M., Kleiman M., Zimmerman S. E., Schlech W., Ho J., White A., Brahmi Z. Immunoglobulin M and G histoplasmal antibody response in histoplasmosis. Am Rev Respir Dis. 1983 Jul;128(1):65–70. doi: 10.1164/arrd.1983.128.1.65. [DOI] [PubMed] [Google Scholar]
  10. Wheat L. J., Slama T. G., Eitzen H. E., Kohler R. B., French M. L., Biesecker J. L. A large urban outbreak of histoplasmosis: clinical features. Ann Intern Med. 1981 Mar;94(3):331–337. doi: 10.7326/0003-4819-94-3-331. [DOI] [PubMed] [Google Scholar]

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