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. 1990 Jan;28(1):87–91. doi: 10.1128/jcm.28.1.87-91.1990

Monoclonal antibodies to Nocardia asteroides and Nocardia brasiliensis antigens.

T Jiménez 1, A M Díaz 1, H Zlotnik 1
PMCID: PMC269542  PMID: 2405017

Abstract

Nocardia asteroides and Nocardia brasiliensis whole-cell extracts were used as antigens to generate monoclonal antibodies (MAbs). Six stable hybrid cell lines secreting anti-Nocardia spp. MAbs were obtained. These were characterized by enzyme-linked immunosorbent assay, Western blot (immunoblot), and immunofluorescence assay. Although all the MAbs exhibited different degrees of cross-reactivity with N. asteroides and N. brasiliensis antigens as well as with culture-filtrate antigens from Mycobacteria spp., they have the potential for use as reagents in the purification of Nocardia antigens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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