Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Jan;28(1):126–127. doi: 10.1128/jcm.28.1.126-127.1990

Evaluation of Bacto TB hydrolysis reagent (Tween 80) for the identification of Branhamella catarrhalis.

M Weiner 1, P D Penha 1
PMCID: PMC269551  PMID: 2105335

Abstract

An investigation of the hydrolysis of Tween 80 reagent by Branhamella catarrhalis and related organisms (Neisseria and Moraxella species) revealed that only B. catarrhalis gave a positive result. A total of 226 strains, including reference organisms and clinical isolates, were studied. B. catarrhalis changed the color of the reagent from amber to pink-red after overnight incubation. We recommend this simple and cost-effective test as an alternative procedure to DNase testing or tributyrin hydrolysis or as a supplemental procedure for the identification of B. catarrhalis in clinical specimens.

Full text

PDF
126

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Davies B. I., Maesen F. P. Branhamella catarrhalis chest infections. Lancet. 1986 Aug 2;2(8501):278–278. doi: 10.1016/s0140-6736(86)92088-x. [DOI] [PubMed] [Google Scholar]
  2. Doern G. V., Miller M. J., Winn R. E. Branhamella (Neisseria) catarrhalis systemic disease in humans. Case reports and review of the literature. Arch Intern Med. 1981 Nov;141(12):1690–1692. [PubMed] [Google Scholar]
  3. Gray L. D., Van Scoy R. E., Anhalt J. P., Yu P. K. Wound infection caused by Branhamella catarrhalis. J Clin Microbiol. 1989 May;27(5):818–820. doi: 10.1128/jcm.27.5.818-820.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Janda W. M., Ruther P. B.CAT CONFIRM, a rapid test for confirmation of Branhamella catarrhalis. J Clin Microbiol. 1989 May;27(5):1130–1131. doi: 10.1128/jcm.27.5.1130-1131.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kovatch A. L., Wald E. R., Michaels R. H. beta-Lactamase-producing Branhamella catarrhalis causing otitis media in children. J Pediatr. 1983 Feb;102(2):261–264. doi: 10.1016/s0022-3476(83)80537-x. [DOI] [PubMed] [Google Scholar]
  6. Wallace R. J., Jr, Musher D. M. In honor of Dr. Sarah Branham, a star is born. The realization of Branhamella catarrhalis as a respiratory pathogen. Chest. 1986 Sep;90(3):447–450. doi: 10.1378/chest.90.3.447. [DOI] [PubMed] [Google Scholar]
  7. Weiner M. Bacto-TB hydrolysis reagent (Tween 80) for identification of Serratia. J Clin Microbiol. 1977 Nov;6(5):536–537. doi: 10.1128/jcm.6.5.536-537.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES