Abstract
An investigation of the hydrolysis of Tween 80 reagent by Branhamella catarrhalis and related organisms (Neisseria and Moraxella species) revealed that only B. catarrhalis gave a positive result. A total of 226 strains, including reference organisms and clinical isolates, were studied. B. catarrhalis changed the color of the reagent from amber to pink-red after overnight incubation. We recommend this simple and cost-effective test as an alternative procedure to DNase testing or tributyrin hydrolysis or as a supplemental procedure for the identification of B. catarrhalis in clinical specimens.
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- Davies B. I., Maesen F. P. Branhamella catarrhalis chest infections. Lancet. 1986 Aug 2;2(8501):278–278. doi: 10.1016/s0140-6736(86)92088-x. [DOI] [PubMed] [Google Scholar]
- Doern G. V., Miller M. J., Winn R. E. Branhamella (Neisseria) catarrhalis systemic disease in humans. Case reports and review of the literature. Arch Intern Med. 1981 Nov;141(12):1690–1692. [PubMed] [Google Scholar]
- Gray L. D., Van Scoy R. E., Anhalt J. P., Yu P. K. Wound infection caused by Branhamella catarrhalis. J Clin Microbiol. 1989 May;27(5):818–820. doi: 10.1128/jcm.27.5.818-820.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Janda W. M., Ruther P. B.CAT CONFIRM, a rapid test for confirmation of Branhamella catarrhalis. J Clin Microbiol. 1989 May;27(5):1130–1131. doi: 10.1128/jcm.27.5.1130-1131.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kovatch A. L., Wald E. R., Michaels R. H. beta-Lactamase-producing Branhamella catarrhalis causing otitis media in children. J Pediatr. 1983 Feb;102(2):261–264. doi: 10.1016/s0022-3476(83)80537-x. [DOI] [PubMed] [Google Scholar]
- Wallace R. J., Jr, Musher D. M. In honor of Dr. Sarah Branham, a star is born. The realization of Branhamella catarrhalis as a respiratory pathogen. Chest. 1986 Sep;90(3):447–450. doi: 10.1378/chest.90.3.447. [DOI] [PubMed] [Google Scholar]
- Weiner M. Bacto-TB hydrolysis reagent (Tween 80) for identification of Serratia. J Clin Microbiol. 1977 Nov;6(5):536–537. doi: 10.1128/jcm.6.5.536-537.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]