Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Jan;28(1):136–139. doi: 10.1128/jcm.28.1.136-139.1990

Numerical analysis of electrophoretic periplasmic protein patterns, a possible marker system for epidemiologic studies.

D Gargallo-Viola 1, D López 1
PMCID: PMC269555  PMID: 2405008

Abstract

The whole-cell and periplasmic protein (PP) compositions of 22 Serratia marcescens isolates were examined. Numerical analysis of whole-cell protein patterns was not a useful procedure for measuring relationships between organisms at the subspecific level. However, there was a very good correlation between electrophoretic PP pattern results and those obtained previously from multilocus enzyme electrophoresis (electrophoretic type) and biotype (D. Gargallo-Viola, J. Clin. Microbiol. 27:860-868, 1989). Clustering of isolates by using PP patterns compared by coefficients based on peak position (Dice coefficient) gave more precise information than that obtained by correlation coefficients. PP patterns appeared to be a useful tool that may be of value for epidemiologic studies.

Full text

PDF
136

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames G. F., Prody C., Kustu S. Simple, rapid, and quantitative release of periplasmic proteins by chloroform. J Bacteriol. 1984 Dec;160(3):1181–1183. doi: 10.1128/jb.160.3.1181-1183.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bermúdez J., López D., Sanahuja A., Viñas M., Lorén J. G. Discriminant analysis of microcalorimetric data of bacterial growth. Can J Microbiol. 1988 Sep;34(9):1058–1062. doi: 10.1139/m88-186. [DOI] [PubMed] [Google Scholar]
  3. Denning D. W., Baker C. J., Troup N. J., Tompkins L. S. Restriction endonuclease analysis of human and bovine group B streptococci for epidemiologic study. J Clin Microbiol. 1989 Jun;27(6):1352–1356. doi: 10.1128/jcm.27.6.1352-1356.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gargallo-Viola D. Enzyme polymorphism, prodigiosin production, and plasmid fingerprints in clinical and naturally occurring isolates of Serratia marcescens. J Clin Microbiol. 1989 May;27(5):860–868. doi: 10.1128/jcm.27.5.860-868.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gargallo D., Lorén J. G., Guinea J., Viñas M. Glucose-6-phosphate dehydrogenase alloenzymes and their relationship to pigmentation in Serratia marcescens. Appl Environ Microbiol. 1987 Aug;53(8):1983–1986. doi: 10.1128/aem.53.8.1983-1986.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gross C. S., Ferguson D. A., Jr, Cummins C. S. Electrophoretic protein patterns and enzyme mobilities in anaerobic coryneforms. Appl Environ Microbiol. 1978 Jun;35(6):1102–1108. doi: 10.1128/aem.35.6.1102-1108.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hancock R. E., Chan L. Outer membranes of environmental isolates of Pseudomonas aeruginosa. J Clin Microbiol. 1988 Nov;26(11):2423–2424. doi: 10.1128/jcm.26.11.2423-2424.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jackman P. J. Classification of Corynebacterium species from axillary skin by numerical analysis of electrophoretic protein patterns. J Med Microbiol. 1982 Nov;15(4):485–492. doi: 10.1099/00222615-15-4-485. [DOI] [PubMed] [Google Scholar]
  9. Kersters K., De Ley J. Classification and identification of bacteria by electrophoresis of their proteins. Soc Appl Bacteriol Symp Ser. 1980;8:273–297. [PubMed] [Google Scholar]
  10. Kersters K., De Ley J. Identification and grouping of bacteria by numerical analysis of their electrophoretic protein patterns. J Gen Microbiol. 1975 Apr;87(2):333–342. doi: 10.1099/00221287-87-2-333. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. López D., Viñas M., Lorén J. G., Bermúdez J. Analysis of microcalorimetric curves for bacterial identification. Can J Microbiol. 1987 Jan;33(1):6–11. doi: 10.1139/m87-002. [DOI] [PubMed] [Google Scholar]
  14. Morris J. A. The use of polyacrylamide gel electrophoresis in taxonomy of Brucella. J Gen Microbiol. 1973 May;76(1):231–237. doi: 10.1099/00221287-76-1-231. [DOI] [PubMed] [Google Scholar]
  15. Mutharia L. M., Nicas T. I., Hancock R. E. Outer membrane proteins of Pseudomonas aeruginosa serotype strains. J Infect Dis. 1982 Dec;146(6):770–779. doi: 10.1093/infdis/146.6.770. [DOI] [PubMed] [Google Scholar]
  16. Owen R. J., Jackman P. J. The similarities between Pseudomonas paucimobilis and allied bacteria derived from analysis of deoxyribonucleic acids and electrophoretic protein patterns. J Gen Microbiol. 1982 Dec;128(12):2945–2954. doi: 10.1099/00221287-128-12-2945. [DOI] [PubMed] [Google Scholar]
  17. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol. 1986 May;51(5):873–884. doi: 10.1128/aem.51.5.873-884.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stock J. B., Rauch B., Roseman S. Periplasmic space in Salmonella typhimurium and Escherichia coli. J Biol Chem. 1977 Nov 10;252(21):7850–7861. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES