Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Feb;28(2):170–176. doi: 10.1128/jcm.28.2.170-176.1990

Species, biotype, and bacteriophage type determinations compared with cell envelope protein profiles for typing Acinetobacter strains.

P J Bouvet 1, S Jeanjean 1, J F Vieu 1, L Dijkshoorn 1
PMCID: PMC269570  PMID: 2179253

Abstract

Species, biotypes, and phage types were determined for 120 Acinetobacter strains from clinical or environmental sources or from culture collections. These characteristics were compared with cell envelope protein profiles obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in previous studies. A considerable heterogeneity of species and types was observed by use of the various methods, in particular among strains from different sources. Acinetobacter baumannii was the most commonly found species in isolates from clinical sources, followed by Acinetobacter species 3. Nine biotypes were observed among A. baumannii strains. Further differentiation within most species and biotypes was achieved by protein profile typing and, to some extent, phage typing. Of 120 strains, 49 (41%) were not typeable by phages. Consistent results for the various classification methods were obtained for strains from common sources. Biotyping seemed an appropriate method for the screening of strains, whereas protein profile and phage typing could serve as additional methods to establish the identity or nonidentity of strains. Results of this study suggest that the combination of the typing methods is useful in epidemiological studies.

Full text

PDF
170

Images in this article

175Image
on p.175
176Image
on p.176

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander M., Ismail F., Jackman P. J., Noble W. C. Fingerprinting Acinetobacter strains from clinical sources by numerical analysis of electrophoretic protein patterns. J Med Microbiol. 1984 Aug;18(1):55–64. doi: 10.1099/00222615-18-1-55. [DOI] [PubMed] [Google Scholar]
  2. Andrews H. J. Acinetobacter bacteriocin typing. J Hosp Infect. 1986 Mar;7(2):169–175. doi: 10.1016/0195-6701(86)90060-5. [DOI] [PubMed] [Google Scholar]
  3. Bergogne-Bérézin E., Joly-Guillou M. L., Vieu J. F. Epidemiology of nosocomial infections due to Acinetobacter calcoaceticus. J Hosp Infect. 1987 Sep;10(2):105–113. doi: 10.1016/0195-6701(87)90135-6. [DOI] [PubMed] [Google Scholar]
  4. Bouvet P. J., Grimont P. A. Identification and biotyping of clinical isolates of Acinetobacter. Ann Inst Pasteur Microbiol. 1987 Sep-Oct;138(5):569–578. doi: 10.1016/0769-2609(87)90042-1. [DOI] [PubMed] [Google Scholar]
  5. Dijkshoorn L., Michel M. F., Degener J. E. Cell envelope protein profiles of Acinetobacter calcoaceticus strains isolated in hospitals. J Med Microbiol. 1987 Jun;23(4):313–319. doi: 10.1099/00222615-23-4-313. [DOI] [PubMed] [Google Scholar]
  6. Dijkshoorn L., Van Vianen W., Degener J. E., Michel M. F. Typing of Acinetobacter calcoaceticus strains isolated from hospital patients by cell envelope protein profiles. Epidemiol Infect. 1987 Dec;99(3):659–667. doi: 10.1017/s0950268800066516. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. French G. L., Casewell M. W., Roncoroni A. J., Knight S., Phillips I. A hospital outbreak of antibiotic-resistant Acinetobacter anitratus: epidemiology and control. J Hosp Infect. 1980 Jun;1(2):125–131. doi: 10.1016/0195-6701(80)90044-4. [DOI] [PubMed] [Google Scholar]
  8. Freney J., Bouvet P. J., Tixier C. Identification et détermination de la sensibilité aux antibiotiques de 31 souches cliniques d'Acinetobacter autres que A. baumannii. Ann Biol Clin (Paris) 1989;47(1):41–44. [PubMed] [Google Scholar]
  9. Glew R. H., Moellering R. C., Jr, Kunz L. J. Infections with Acinetobacter calcoaceticus (Herellea vaginicola): clinical and laboratory studies. Medicine (Baltimore) 1977 Mar;56(2):79–97. doi: 10.1097/00005792-197703000-00001. [DOI] [PubMed] [Google Scholar]
  10. Juni E. Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus. J Bacteriol. 1972 Nov;112(2):917–931. doi: 10.1128/jb.112.2.917-931.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Marcus B. B., Samuels S. B., Pittman B., Cherry W. B. A serologic study of Herellea vaginicola and its identification by immunofluorescent staining. Am J Clin Pathol. 1969 Sep;52(3):309–319. doi: 10.1093/ajcp/52.3.309. [DOI] [PubMed] [Google Scholar]
  12. Santos Ferreira M. O., Vieu J. F., Klein B. Phage-types and susceptibility to 26 antibiotics of nosocomial strains of Acinetobacter isolated in Portugal. J Int Med Res. 1984;12(6):364–368. doi: 10.1177/030006058401200609. [DOI] [PubMed] [Google Scholar]
  13. Vieu J. F., Bergogne-Berezin E., Joly M. L., Berthelot G., Fichelle A., Prevost C. Epidémiologie d'Acinetobacter calcoaceticus. Nouv Presse Med. 1980 Dec 6;9(46):3551–3552. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES