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. 1990 Feb;28(2):201–206. doi: 10.1128/jcm.28.2.201-206.1990

Cytopathic astrovirus isolated from porcine acute gastroenteritis in an established cell line derived from porcine embryonic kidney.

M Shimizu 1, J Shirai 1, M Narita 1, T Yamane 1
PMCID: PMC269575  PMID: 2107200

Abstract

A cytopathic astrovirus was isolated from pigs with acute diarrhea in an established cell line that was derived from porcine embryonic kidneys with the aid of trypsin. The virus showed a distinct cytopathic effect characterized by an enlargement of cells and the appearance of fine granules in the cytoplasm. Porcine astrovirus was shown to have an RNA genome, as determined by the effect of 5-iodo-2'-deoxyuridine on its replication, and five polypeptides with molecular masses of 13,000, 30,000, 31,000, 36,000, and 39,000 daltons; and it was shown to be stable to lipid solvents and heating at 50 degrees C for 30 min but somewhat labile to acid (pH 3.0). The buoyant density of the isolate determined in CsCl was 1.35 g/ml. Seroconversion to the virus was evident in the paired serum specimens obtained from pigs with diarrhea that were housed at the farm where the disease occurred. The neutralization test on serum specimens collected randomly from 128 adult pigs of eight herds revealed that 50 of the serum specimens were positive for antibody to porcine astrovirus, although there was considerable variation in the prevalence among herds, ranging from 0 to 83%. Hysterectomy-produced, colostrum-deprived, 4-day-old pigs developed mild diarrhea after oral exposure to porcine astrovirus propagated in the cell culture; and the virus was isolated again from diarrheal stool specimens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bridger J. C. Detection by electron microscopy of caliciviruses, astroviruses and rotavirus-like particles in the faeces of piglets with diarrhoea. Vet Rec. 1980 Dec 6;107(23):532–533. [PubMed] [Google Scholar]
  2. Dolin R., Treanor J. J., Madore H. P. Novel agents of viral enteritis in humans. J Infect Dis. 1987 Mar;155(3):365–376. doi: 10.1093/infdis/155.3.365. [DOI] [PubMed] [Google Scholar]
  3. Gray J. J., Wreghitt T. G., Cubitt W. D., Elliot P. R. An outbreak of gastroenteritis in a home for the elderly associated with astrovirus type 1 and human calicivirus. J Med Virol. 1987 Dec;23(4):377–381. doi: 10.1002/jmv.1890230410. [DOI] [PubMed] [Google Scholar]
  4. Harbour D. A., Ashley C. R., Williams P. D., Gruffydd-Jones T. J. Natural and experimental astrovirus infection of cats. Vet Rec. 1987 Jun 6;120(23):555–557. doi: 10.1136/vr.120.23.555. [DOI] [PubMed] [Google Scholar]
  5. Herring A. J., Gray E. W., Snodgrass D. R. Purification and characterization of ovine astrovirus. J Gen Virol. 1981 Mar;53(Pt 1):47–55. doi: 10.1099/0022-1317-53-1-47. [DOI] [PubMed] [Google Scholar]
  6. Hoshino Y., Zimmer J. F., Moise N. S., Scott F. W. Detection of astroviruses in feces of a cat with diarrhea. Brief report. Arch Virol. 1981;70(4):373–376. doi: 10.1007/BF01320252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Konno T., Suzuki H., Ishida N., Chiba R., Mochizuki K., Tsunoda A. Astrovirus-associated epidemic gastroenteritis in Japan. J Med Virol. 1982;9(1):11–17. doi: 10.1002/jmv.1890090103. [DOI] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Lee T. W., Kurtz J. B. Human astrovirus serotypes. J Hyg (Lond) 1982 Dec;89(3):539–540. doi: 10.1017/s0022172400071114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lee T. W., Kurtz J. B. Serial propagation of astrovirus in tissue culture with the aid of trypsin. J Gen Virol. 1981 Dec;57(Pt 2):421–424. doi: 10.1099/0022-1317-57-2-421. [DOI] [PubMed] [Google Scholar]
  11. Madeley C. R., Cosgrove B. P. Letter: 28 nm particles in faeces in infantile gastroenteritis. Lancet. 1975 Sep 6;2(7932):451–452. doi: 10.1016/s0140-6736(75)90858-2. [DOI] [PubMed] [Google Scholar]
  12. Marshall J. A., Kennett M. L., Rodger S. M., Studdert M. J., Thompson W. L., Gust I. D. Virus and virus-like particles in the faeces of cats with and without diarrhoea. Aust Vet J. 1987 Apr;64(4):100–105. doi: 10.1111/j.1751-0813.1987.tb09638.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Riepenhoff-Talty M., Saif L. J., Barrett H. J., Suzuki H., Ogra P. L. Potential spectrum of etiological agents of viral enteritis in hospitalized infants. J Clin Microbiol. 1983 Feb;17(2):352–356. doi: 10.1128/jcm.17.2.352-356.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Saif L. J., Bohl E. H., Theil K. W., Cross R. F., House J. A. Rotavirus-like, calicivirus-like, and 23-nm virus-like particles associated with diarrhea in young pigs. J Clin Microbiol. 1980 Jul;12(1):105–111. doi: 10.1128/jcm.12.1.105-111.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shirai J., Shimizu M., Fukusho A. Coronavirus-, calicivirus-, and astrovirus-like particles associated with acute porcine gastroenteritis. Nihon Juigaku Zasshi. 1985 Dec;47(6):1023–1026. doi: 10.1292/jvms1939.47.1023. [DOI] [PubMed] [Google Scholar]
  16. Snodgrass D. R., Gray E. W. Detection and transmission of 30 nm virus particles (astroviruses) in faeces of lambs with diarrhoea. Arch Virol. 1977;55(4):287–291. doi: 10.1007/BF01315050. [DOI] [PubMed] [Google Scholar]
  17. Sugimori T., Morimoto T., Miura Y., Sazawa H. Hemagglutin of Japanese encephalitis virus produced in embryonic swine kidney cell cultures. Natl Inst Anim Health Q (Tokyo) 1969 Summer;9(2):55–64. [PubMed] [Google Scholar]
  18. Williams F. P., Jr Astrovirus-like, coronavirus-like, and parvovirus-like particles detected in the diarrheal stools of beagle pups. Arch Virol. 1980;66(3):215–226. doi: 10.1007/BF01314735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Woode G. N., Bridger J. C. Isolation of small viruses resembling astroviruses and caliciviruses from acute enteritis of calves. J Med Microbiol. 1978 Nov;11(4):441–452. doi: 10.1099/00222615-11-4-441. [DOI] [PubMed] [Google Scholar]
  20. Woode G. N., Pohlenz J. F., Gourley N. E., Fagerland J. A. Astrovirus and Breda virus infections of dome cell epithelium of bovine ileum. J Clin Microbiol. 1984 May;19(5):623–630. doi: 10.1128/jcm.19.5.623-630.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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