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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Feb;28(2):319–323. doi: 10.1128/jcm.28.2.319-323.1990

Species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria.

K Dix 1, S M Watanabe 1, S McArdle 1, D I Lee 1, C Randolph 1, B Moncla 1, D E Schwartz 1
PMCID: PMC269598  PMID: 2312676

Abstract

Oligodeoxynucleotide probes were developed for identification of the periodontal bacteria Actinobacillus actinomycetemcomitans, Bacteroides gingivalis, B. intermedius types I and II, B. forsythus, Eikenella corrodens, Fusobacterium nucleatum, Haemophilus aphrophilus, Streptococcus intermedius, and Wolinella recta. Probes were designed by sequencing the 16S rRNA for each bacterium, identifying hypervariable regions, and chemically synthesizing species-specific probes. These probes were specific when tested against a panel of nucleic acids from closely related bacteria.

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Selected References

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