Abstract
Six different oligonucleotide probes complementary to the hypervariable regions of 16S rRNA of Bacteroides gingivalis were tested for specificity and sensitivity against 77 field strains of B. gingivalis and 105 strains of 12 other Bacteroides species. The data demonstrated that these probes were very specific (range, 0.85 to 1.00) and sensitive (1.00). Some limited cross-reactions with other Bacteroides species were observed. Four of these probes should be useful for rapid detection and identification of B. gingivalis.
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bonta Y., Zambon J. J., Genco R. J., Neiders M. E. Rapid identification of periodontal pathogens in subgingival plaque: comparison of indirect immunofluorescence microscopy with bacterial culture for detection of Actinobacillus actinomycetemcomitans. J Dent Res. 1985 May;64(5):793–798. doi: 10.1177/00220345850640050201. [DOI] [PubMed] [Google Scholar]
- Conway de Macario E., Macario A. J. Monoclonal antibodies for bacterial identification and taxonomy: 1985 and beyond. Clin Lab Med. 1985 Sep;5(3):531–544. [PubMed] [Google Scholar]
- Dix K., Watanabe S. M., McArdle S., Lee D. I., Randolph C., Moncla B., Schwartz D. E. Species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria. J Clin Microbiol. 1990 Feb;28(2):319–323. doi: 10.1128/jcm.28.2.319-323.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holdeman L. V., Cato E. P., Moore W. E. Taxonomy of anaerobes: present state of the art. Rev Infect Dis. 1984 Mar-Apr;6 (Suppl 1):S3–10. doi: 10.1093/clinids/6.supplement_1.s3. [DOI] [PubMed] [Google Scholar]
- Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A. 1985 Oct;82(20):6955–6959. doi: 10.1073/pnas.82.20.6955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loesche W. J. The identification of bacteria associated with periodontal disease and dental caries by enzymatic methods. Oral Microbiol Immunol. 1986 Nov;1(1):65–72. doi: 10.1111/j.1399-302x.1986.tb00322.x. [DOI] [PubMed] [Google Scholar]
- Mayrand D., Holt S. C. Biology of asaccharolytic black-pigmented Bacteroides species. Microbiol Rev. 1988 Mar;52(1):134–152. doi: 10.1128/mr.52.1.134-152.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moncla B. J., Strockbine L., Braham P., Karlinsey J., Roberts M. C. The use of whole-cell DNA probes for the identification of Bacteroides intermedius isolates in a dot blot assay. J Dent Res. 1988 Oct;67(10):1267–1270. doi: 10.1177/00220345880670100401. [DOI] [PubMed] [Google Scholar]
- Moore W. E. Microbiology of periodontal disease. J Periodontal Res. 1987 Sep;22(5):335–341. doi: 10.1111/j.1600-0765.1987.tb01595.x. [DOI] [PubMed] [Google Scholar]
- Roberts M. C., Hillier S. L., Schoenknecht F. D., Holmes K. K. Comparison of gram stain, DNA probe, and culture for the identification of species of Mobiluncus in female genital specimens. J Infect Dis. 1985 Jul;152(1):74–77. doi: 10.1093/infdis/152.1.74. [DOI] [PubMed] [Google Scholar]
- Roberts M. C., Moncla B., Kenny G. E. Chromosomal DNA probes for the identification of Bacteroides species. J Gen Microbiol. 1987 Jun;133(6):1423–1430. doi: 10.1099/00221287-133-6-1423. [DOI] [PubMed] [Google Scholar]
- Tenover F. C. Diagnostic deoxyribonucleic acid probes for infectious diseases. Clin Microbiol Rev. 1988 Jan;1(1):82–101. doi: 10.1128/cmr.1.1.82. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tompkins L. S. Recombinant DNA and other direct specimen identification techniques. Clin Lab Med. 1985 Mar;5(1):99–107. [PubMed] [Google Scholar]
- Zambon J. J., Slots J., Genco R. J. Serology of oral Actinobacillus actinomycetemcomitans and serotype distribution in human periodontal disease. Infect Immun. 1983 Jul;41(1):19–27. doi: 10.1128/iai.41.1.19-27.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]