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. 1990 Feb;28(2):358–360. doi: 10.1128/jcm.28.2.358-360.1990

Reactivity of coagulase-negative staphylococci isolated from cow and goat milk with monoclonal antibodies to Staphylococcus aureus capsular polysaccharide types 5 and 8.

B Poutrel 1, C Mendolia 1, L Sutra 1, J M Fournier 1
PMCID: PMC269607  PMID: 2312680

Abstract

Monoclonal antibodies to Staphylococcus aureus capsular polysaccharide types 5 and 8 were used in an enzyme-linked immunosorbent assay to serotype 74 and 42 coagulase-negative isolates from cow and goat milk, respectively. Eighteen (15.5%) isolates were typable: 13 Staphylococcus haemolyticus, 1 S. hyicus, 1 S. simulans, and 1 S. warneri from bovine origin and 2 S. lentus from caprine origin. Type 5 was predominant, accounting for about 89% of typable isolates. Reactivity with monoclonal antibodies varied considerably according to isolates. The significance and the potential importance of these findings are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. C., Wilson C. D. Encapsulated, coagulase-negative strain of Staphylococcus simulans. Infect Immun. 1981 Jul;33(1):304–308. doi: 10.1128/iai.33.1.304-308.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arbeit R. D., Karakawa W. W., Vann W. F., Robbins J. B. Predominance of two newly described capsular polysaccharide types among clinical isolates of Staphylococcus aureus. Diagn Microbiol Infect Dis. 1984 Apr;2(2):85–91. doi: 10.1016/0732-8893(84)90002-6. [DOI] [PubMed] [Google Scholar]
  3. Boutonnier A., Nato F., Bouvet A., Lebrun L., Audurier A., Mazie J. C., Fournier J. M. Direct testing of blood culture for detection of the serotype 5 and 8 capsular polysaccharides of Staphylococcus aureus. J Clin Microbiol. 1989 May;27(5):989–993. doi: 10.1128/jcm.27.5.989-993.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Christensen G. D., Parisi J. T., Bisno A. L., Simpson W. A., Beachey E. H. Characterization of clinically significant strains of coagulase-negative staphylococci. J Clin Microbiol. 1983 Aug;18(2):258–269. doi: 10.1128/jcm.18.2.258-269.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Devriese L. A., Schleifer K. H., Adegoke G. O. Identification of coagulase-negative staphylococci from farm animals. J Appl Bacteriol. 1985 Jan;58(1):45–55. doi: 10.1111/j.1365-2672.1985.tb01428.x. [DOI] [PubMed] [Google Scholar]
  6. Fournier J. M., Hannon K., Moreau M., Karakawa W. W., Vann W. F. Isolation of type 5 capsular polysaccharide from Staphylococcus aureus. Ann Inst Pasteur Microbiol. 1987 Sep-Oct;138(5):561–567. doi: 10.1016/0769-2609(87)90041-x. [DOI] [PubMed] [Google Scholar]
  7. Fournier J. M., Vann W. F., Karakawa W. W. Purification and characterization of Staphylococcus aureus type 8 capsular polysaccharide. Infect Immun. 1984 Jul;45(1):87–93. doi: 10.1128/iai.45.1.87-93.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Karakawa W. W., Fournier J. M., Vann W. F., Arbeit R., Schneerson R. S., Robbins J. B. Method for the serological typing of the capsular polysaccharides of Staphylococcus aureus. J Clin Microbiol. 1985 Sep;22(3):445–447. doi: 10.1128/jcm.22.3.445-447.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Karakawa W. W., Sutton A., Schneerson R., Karpas A., Vann W. F. Capsular antibodies induce type-specific phagocytosis of capsulated Staphylococcus aureus by human polymorphonuclear leukocytes. Infect Immun. 1988 May;56(5):1090–1095. doi: 10.1128/iai.56.5.1090-1095.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kristinsson K. G., Spencer R. C. Slime production as a marker for clinically significant infections with coagulase-negative staphylococci. J Infect Dis. 1986 Oct;154(4):728–729. doi: 10.1093/infdis/154.4.728. [DOI] [PubMed] [Google Scholar]
  11. Lerondelle C., Poutrel B. Characteristics of non-clinical mammary infections of goats. Ann Rech Vet. 1984;15(1):105–112. [PubMed] [Google Scholar]
  12. Nelles M. J., Niswander C. A., Karakawa W. W., Vann W. F., Arbeit R. D. Reactivity of type-specific monoclonal antibodies with Staphylococcus aureus clinical isolates and purified capsular polysaccharide. Infect Immun. 1985 Jul;49(1):14–18. doi: 10.1128/iai.49.1.14-18.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Poutrel B., Boutonnier A., Sutra L., Fournier J. M. Prevalence of capsular polysaccharide types 5 and 8 among Staphylococcus aureus isolates from cow, goat, and ewe milk. J Clin Microbiol. 1988 Jan;26(1):38–40. doi: 10.1128/jcm.26.1.38-40.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Poutrel B. Udder infection of goats by coagulase-negative staphylococci. Vet Microbiol. 1984 Apr;9(2):131–137. doi: 10.1016/0378-1135(84)90028-2. [DOI] [PubMed] [Google Scholar]
  15. Rainard P., Poutrel B. Dynamics of nonclinical bovine intramammary infections with major and minor pathogens. Am J Vet Res. 1982 Dec;43(12):2143–2146. [PubMed] [Google Scholar]
  16. Sompolinsky D., Samra Z., Karakawa W. W., Vann W. F., Schneerson R., Malik Z. Encapsulation and capsular types in isolates of Staphylococcus aureus from different sources and relationship to phage types. J Clin Microbiol. 1985 Nov;22(5):828–834. doi: 10.1128/jcm.22.5.828-834.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Timms L. L., Schultz L. H. Dynamics and significance of coagulase-negative staphylococcal intramammary infections. J Dairy Sci. 1987 Dec;70(12):2648–2657. doi: 10.3168/jds.S0022-0302(87)80335-1. [DOI] [PubMed] [Google Scholar]
  18. Yamada T., Ichiman Y., Yoshida K. Possible common biological and immunological properties for detecting encapsulated strains of Staphylococcus epidermidis. J Clin Microbiol. 1988 Oct;26(10):2167–2172. doi: 10.1128/jcm.26.10.2167-2172.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yoshida K., Ichiman Y., Umeda A. Cross protection between an encapsulated strain of Staphylococcus hyicus and encapsulated strains of Staphylococcus aureus. J Appl Bacteriol. 1988 Dec;65(6):491–499. doi: 10.1111/j.1365-2672.1988.tb01922.x. [DOI] [PubMed] [Google Scholar]
  20. Yoshida K., Ichiman Y., Umeda A., Takeuchi S. Demonstration of capsule in a strain of Staphylococcus hyicus by an electron microscope. J Appl Bacteriol. 1988 Jun;64(6):483–486. doi: 10.1111/j.1365-2672.1988.tb02439.x. [DOI] [PubMed] [Google Scholar]
  21. Yoshida K., Minegishi Y. Isolation of an encapsulated strain of Staphylococcus epidermidis. J Appl Bacteriol. 1979 Oct;47(2):299–301. doi: 10.1111/j.1365-2672.1979.tb01758.x. [DOI] [PubMed] [Google Scholar]

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