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. 1990 Feb;28(2):379–382. doi: 10.1128/jcm.28.2.379-382.1990

Enzyme-linked immunosorbent assay for distinguishing serological responses of lepromatous and tuberculoid leprosies to the 29/33-kilodalton doublet and 64-kilodalton antigens of Mycobacterium tuberculosis.

P K Das 1, A Rambukkana 1, J G Baas 1, D G Groothuis 1, M Halperin 1
PMCID: PMC269615  PMID: 2107205

Abstract

Immunoblot assays for the antibodies to Mycobacterium tuberculosis sonic extracts showed that all serum specimens of 40 lepromatous and of 28 tuberculoid leprosy patients reacted in a significant manner to 29/33-kilodalton (kDa) doublet and 64-kDa antigens, respectively. By using an enzyme-linked immunosorbent assay, we observed a significantly high immunoglobulin G antibody titer to the purified M. tuberculosis 29/33-kDa doublet and 64-kDa antigens in lepromatous and tuberculoid leprosy patients, respectively, as compared with normal subjects and tuberculosis patients. This enzyme-linked immunosorbent assay serology may be useful for distinguishing two polar types of leprosy and for diagnosing leprosy in general.

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Selected References

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  1. Bothamley G., Swanson Beck J., Agusni I., Ilias M. I., Kardjito T., Grange J. M., Ivanyi J. Antibodies to Mycobacterium tuberculosis in leprosy. Lancet. 1987 May 9;1(8541):1098–1098. doi: 10.1016/s0140-6736(87)90532-0. [DOI] [PubMed] [Google Scholar]
  2. Britton W. J., Hellqvist L., Garsia R. J., Basten A. Antigens of Mycobacterium leprae identified by immunoprecipitation with sera from leprosy and tuberculosis patients. Clin Exp Immunol. 1988 Mar;71(3):394–398. [PMC free article] [PubMed] [Google Scholar]
  3. Daniel T. M., Debanne S. M. The serodiagnosis of tuberculosis and other mycobacterial diseases by enzyme-linked immunosorbent assay. Am Rev Respir Dis. 1987 May;135(5):1137–1151. doi: 10.1164/arrd.1987.135.5.1137. [DOI] [PubMed] [Google Scholar]
  4. Daniel T. M., Janicki B. W. Mycobacterial antigens: a review of their isolation, chemistry, and immunological properties. Microbiol Rev. 1978 Mar;42(1):84–113. doi: 10.1128/mr.42.1.84-113.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Das P. K., Ibrahim A. A., Binkhuysen F., Thi Hué P., Qui T. H., Rangarajan R., Ruitenberg E. J. The use of subcellular components of BCG for studying host-Mycobacterium interaction in relation to leprosy. Ann Immunol (Paris) 1982 Jul-Aug;133D(1):41–59. [PubMed] [Google Scholar]
  6. Das P. K., Rambukkana A., Bass J. G., Groothuis D. G., Kok A., Halperin M. Identification of mycobacterial antigens for "ELISA" serology in the diagnosis of leprosy and tuberculosis. Acta Leprol. 1989;7 (Suppl 1):117–120. [PubMed] [Google Scholar]
  7. Das P. K., Tulp A. Use of a unit gravity sedimentation chamber for the purification of Mycobacterium leprae. Ann Microbiol (Paris) 1982 Nov-Dec;133(3):389–400. [PubMed] [Google Scholar]
  8. Ehrenberg J. P., Gebre N. Analysis of the antigenic profile of Mycobacterium leprae: cross-reactive and unique specificities of human and rabbit antibodies. Scand J Immunol. 1987 Dec;26(6):673–681. doi: 10.1111/j.1365-3083.1987.tb02303.x. [DOI] [PubMed] [Google Scholar]
  9. Fine P. Leprosy and tuberculosis--an epidemiological comparison. Tubercle. 1984 Jun;65(2):137–153. doi: 10.1016/0041-3879(84)90067-9. [DOI] [PubMed] [Google Scholar]
  10. Gaylord H., Brennan P. J., Young D. B., Buchanan T. M. Most Mycobacterium leprae carbohydrate-reactive monoclonal antibodies are directed to lipoarabinomannan. Infect Immun. 1987 Nov;55(11):2860–2863. doi: 10.1128/iai.55.11.2860-2863.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Harboe M. Immunological aspects of leprosy: ten years' activity at the Armauer Hansen Research Institute and Prospects for further work. Int J Lepr Other Mycobact Dis. 1980 Jun;48(2):193–205. [PubMed] [Google Scholar]
  12. Ivanyi J., Sinha S., Aston R., Cussell D., Keen M., Sengupta U. Definition of species specific and cross-reactive antigenic determinants of Mycobacterium leprae using monoclonal antibodies. Clin Exp Immunol. 1983 Jun;52(3):528–536. [PMC free article] [PubMed] [Google Scholar]
  13. Kaufmann S. H. Immunology of leprosy: new findings, future perspectives. Microb Pathog. 1986 Apr;1(2):107–114. doi: 10.1016/0882-4010(86)90013-6. [DOI] [PubMed] [Google Scholar]
  14. Klatser P. R., De Wit M. Y., Kolk A. H. An ELISA-inhibition test using monoclonal antibody for the serology of leprosy. Clin Exp Immunol. 1985 Dec;62(3):468–473. [PMC free article] [PubMed] [Google Scholar]
  15. Klatser P. R., van Rens M. M., Eggelte T. A. Immunochemical characterization of Mycobacterium leprae antigens by the SDS-polyacrylamide gel electrophoresis immunoperoxidase technique (SGIP) using patients' sera. Clin Exp Immunol. 1984 Jun;56(3):537–544. [PMC free article] [PubMed] [Google Scholar]
  16. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  17. Rumschlag H. S., Shinnick T. M., Cohen M. L. Serological responses of patients with lepromatous and tuberculoid leprosy to 30-, 31-, and 32-kilodalton antigens of Mycobacterium tuberculosis. J Clin Microbiol. 1988 Oct;26(10):2200–2202. doi: 10.1128/jcm.26.10.2200-2202.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shoenfeld Y., Isenberg D. A. Mycobacteria and autoimmunity. Immunol Today. 1988 Jun;9(6):178–182. doi: 10.1016/0167-5699(88)91294-7. [DOI] [PubMed] [Google Scholar]
  19. Sinha S., Sengupta U., Ramu G., Ivanyi J. A serological test for leprosy based on competitive inhibition of monoclonal antibody binding to the MY2a determinant of Mycobacterium leprae. Trans R Soc Trop Med Hyg. 1983;77(6):869–871. doi: 10.1016/0035-9203(83)90309-7. [DOI] [PubMed] [Google Scholar]
  20. Stanford J. L. Leprosy research, present and future. Acta Leprol. 1984 Oct-Dec;(95):421–425. [PubMed] [Google Scholar]
  21. de Wit M. Y., Klatser P. R. Purification and characterization of a 36 kDa antigen of Mycobacterium leprae. J Gen Microbiol. 1988 Jun;134(6):1541–1548. doi: 10.1099/00221287-134-6-1541. [DOI] [PubMed] [Google Scholar]

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