Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Feb;28(2):393–394. doi: 10.1128/jcm.28.2.393-394.1990

Rapid detection of Pneumocystis carinii in bronchoalveolar lavage samples by using Cellufluor staining.

V S Baselski 1, M K Robison 1, L W Pifer 1, D R Woods 1
PMCID: PMC269620  PMID: 1690218

Abstract

Cellufluor (Calcofluor white) has been found to be a useful, rapid chemofluorescent stain for detection of Pneumocystis carinii cysts in bronchoalveolar lavage samples. When compared with toluidine blue O and Giemsa stains on 45 specimens (22 positive and 23 negative), the sensitivity and specificity of the Cellufluor stain were 95 and 100%, respectively.

Full text

PDF
393

Images in this article

394Image
on p.394

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bedrossian C. W., Mason M. R., Gupta P. K. Rapid cytologic diagnosis of Pneumocystis: a comparison of effective techniques. Semin Diagn Pathol. 1989 Aug;6(3):245–261. [PubMed] [Google Scholar]
  2. Blumenfeld W., Griffiss J. M. Pneumocystis carinii in sputum. Comparable efficacy of screening stains and determination of cyst density. Arch Pathol Lab Med. 1988 Aug;112(8):816–820. [PubMed] [Google Scholar]
  3. Broaddus C., Dake M. D., Stulbarg M. S., Blumenfeld W., Hadley W. K., Golden J. A., Hopewell P. C. Bronchoalveolar lavage and transbronchial biopsy for the diagnosis of pulmonary infections in the acquired immunodeficiency syndrome. Ann Intern Med. 1985 Jun;102(6):747–752. doi: 10.7326/0003-4819-102-6-747. [DOI] [PubMed] [Google Scholar]
  4. CHALVARDJIAN A. M., GRAWE L. A. A NEW PROCEDURE FOR THE IDENTIFICATION OF PNEUMOCYSTIS CARINII CYSTS IN TISSUE SECTIONS AND SMEARS. J Clin Pathol. 1963 Jul;16:383–384. doi: 10.1136/jcp.16.4.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gill V. J., Evans G., Stock F., Parrillo J. E., Masur H., Kovacs J. A. Detection of Pneumocystis carinii by fluorescent-antibody stain using a combination of three monoclonal antibodies. J Clin Microbiol. 1987 Oct;25(10):1837–1840. doi: 10.1128/jcm.25.10.1837-1840.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gill V. J., Nelson N. A., Stock F., Evans G. Optimal use of the cytocentrifuge for recovery and diagnosis of Pneumocystis carinii in bronchoalveolar lavage and sputum specimens. J Clin Microbiol. 1988 Sep;26(9):1641–1644. doi: 10.1128/jcm.26.9.1641-1644.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gutierrez Y. The biology of Pneumocystis carinii. Semin Diagn Pathol. 1989 Aug;6(3):203–211. [PubMed] [Google Scholar]
  8. Hughes J., McCully M. E. The use of an optical brightener in the study of plant structure. Stain Technol. 1975 Sep;50(5):319–329. doi: 10.3109/10520297509117082. [DOI] [PubMed] [Google Scholar]
  9. Hughes W. T., Price R. A., Kim H. K., Coburn T. P., Grigsby D., Feldman S. Pneumocystis carinii pneumonitis in children with malignancies. J Pediatr. 1973 Mar;82(3):404–415. doi: 10.1016/s0022-3476(73)80113-1. [DOI] [PubMed] [Google Scholar]
  10. Kahn F. W., Jones J. M. Analysis of bronchoalveolar lavage specimens from immunocompromised patients with a protocol applicable in the microbiology laboratory. J Clin Microbiol. 1988 Jun;26(6):1150–1155. doi: 10.1128/jcm.26.6.1150-1155.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kim H. K., Hughes W. T. Comparison of methods for identification of Pneumocystis carinii in pulmonary aspirates. Am J Clin Pathol. 1973 Oct;60(4):462–466. doi: 10.1093/ajcp/60.4.462. [DOI] [PubMed] [Google Scholar]
  12. Linder J., Radio S. J. Immunohistochemistry of Pneumocystis carinii. Semin Diagn Pathol. 1989 Aug;6(3):238–244. [PubMed] [Google Scholar]
  13. Maeda H., Ishida N. Specificity of binding of hexopyranosyl polysaccharides with fluorescent brightener. J Biochem. 1967 Aug;62(2):276–278. doi: 10.1093/oxfordjournals.jbchem.a128660. [DOI] [PubMed] [Google Scholar]
  14. Monheit J. E., Cowan D. F., Moore D. G. Rapid detection of fungi in tissues using calcofluor white and fluorescence microscopy. Arch Pathol Lab Med. 1984 Aug;108(8):616–618. [PubMed] [Google Scholar]
  15. Stover D. E., Zaman M. B., Hajdu S. I., Lange M., Gold J., Armstrong D. Bronchoalveolar lavage in the diagnosis of diffuse pulmonary infiltrates in the immunosuppressed host. Ann Intern Med. 1984 Jul;101(1):1–7. doi: 10.7326/0003-4819-101-1-1. [DOI] [PubMed] [Google Scholar]
  16. Walzer P. D. Diagnosis of Pneumocystis carinii pneumonia. J Infect Dis. 1988 Apr;157(4):629–632. doi: 10.1093/infdis/157.4.629. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES