Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Mar;28(3):430–434. doi: 10.1128/jcm.28.3.430-434.1990

Use of Haemophilus test medium for broth microdilution antimicrobial susceptibility testing of Streptococcus pneumoniae.

J H Jorgensen 1, L A Maher 1, A W Howell 1
PMCID: PMC269637  PMID: 2324270

Abstract

A recently described medium (Haemophilus test medium [HTM]) for antimicrobial susceptibility testing of Haemophilus influenzae was evaluated in this study for broth microdilution testing of Streptococcus pneumoniae. A total of 137 clinical isolates was tested against 11 antimicrobial agents, using Mueller-Hinton broth supplemented with 3% lysed horse blood in parallel with HTM. Inocula of 5 X 10(5) CFU/ml and incubation for 20 to 24 h were used with both media. All isolates of S. pneumoniae produced acceptable growth in both media, and MICs determined in HTM agreed closely with those determined in lysed horse blood. Drugs which provided a MIC within 1 log2 concentration difference in both media included penicillin (100%), ampicillin (98.0%), amoxicillin-clavulanate (100%), ampicillin-sulbactam (100%), cephalexin (98.9%), cefaclor (96.8%), cefuroxime (99.0%), chloramphenicol (96.2%), tetracycline (96.2%), and erythromycin (100%). HTM MICs with trimethoprim-sulfamethoxazole were 1 to 2 log2 concentration increments higher in 92.0% of isolates than MICs determined in lysed horse blood. Based on the results of this study, HTM appears to represent a promising alternative medium for broth microdilution susceptibility testing of S. pneumoniae.

Full text

PDF
430

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. D'Amato R. F., Swenson J. M., McKinley G. A., Hochstein L., Wallman A. A., Cleri D. J., Mastellone A. J., Fredericks L., Gonzalez L., Pincus D. H. Quantitative antimicrobial susceptibility test for Streptococcus pneumoniae using inoculum supplemented with whole defibrinated sheep blood. J Clin Microbiol. 1987 Sep;25(9):1753–1756. doi: 10.1128/jcm.25.9.1753-1756.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dang-Van A., Tiraby G., Acar J. F., Shaw W. V., Bouanchaud D. H. Chloramphenicol resistance in Streptococcus pneumoniae: enzymatic acetylation and possible plasmid linkage. Antimicrob Agents Chemother. 1978 Apr;13(4):577–583. doi: 10.1128/aac.13.4.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Istre G. R., Humphreys J. T., Albrecht K. D., Thornsberry C., Swenson J. M., Hopkins R. S. Chloramphenicol and penicillin resistance in pneumococci isolated from blood and cerebrospinal fluid: a prevalence study in metropolitan Denver. J Clin Microbiol. 1983 Mar;17(3):472–475. doi: 10.1128/jcm.17.3.472-475.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jackson M. A., Shelton S., Nelson J. D., McCracken G. H., Jr Relatively penicillin-resistant pneumococcal infections in pediatric patients. Pediatr Infect Dis. 1984 Mar-Apr;3(2):129–132. doi: 10.1097/00006454-198403000-00010. [DOI] [PubMed] [Google Scholar]
  5. Jacobs M. R., Koornhof H. J., Robins-Browne R. M., Stevenson C. M., Vermaak Z. A., Freiman I., Miller G. B., Witcomb M. A., Isaäcson M., Ward J. I. Emergence of multiply resistant pneumococci. N Engl J Med. 1978 Oct 5;299(14):735–740. doi: 10.1056/NEJM197810052991402. [DOI] [PubMed] [Google Scholar]
  6. Jacobs M. R., Mithal Y., Robins-Browne R. M., Gaspar M. N., Koornhof H. J. Antimicrobial susceptibility testing of pneumococci: determination of Kirby-Bauer breakpoints for penicillin G, erythromycin, clindamycin, tetracycline, chloramphenicol, and rifampin. Antimicrob Agents Chemother. 1979 Aug;16(2):190–197. doi: 10.1128/aac.16.2.190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jones R. N., Edson D. C. Special topics in antimicrobial susceptibility testing: test accuracy against methicillin-resistant Staphylococcus aureus, pneumococci, and the sensitivity of beta-lactamase methods. Am J Clin Pathol. 1983 Oct;80(4 Suppl):609–614. [PubMed] [Google Scholar]
  8. Jorgensen J. H., Redding J. S., Maher L. A., Howell A. W. Improved medium for antimicrobial susceptibility testing of Haemophilus influenzae. J Clin Microbiol. 1987 Nov;25(11):2105–2113. doi: 10.1128/jcm.25.11.2105-2113.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Klugman K. P., Koornhof H. J. Drug resistance patterns and serogroups or serotypes of pneumococcal isolates from cerebrospinal fluid or blood, 1979-1986. J Infect Dis. 1988 Nov;158(5):956–964. doi: 10.1093/infdis/158.5.956. [DOI] [PubMed] [Google Scholar]
  10. Markiewicz Z., Tomasz A. Variation in penicillin-binding protein patterns of penicillin-resistant clinical isolates of pneumococci. J Clin Microbiol. 1989 Mar;27(3):405–410. doi: 10.1128/jcm.27.3.405-410.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Matthews H. W., Baker C. N., Thornsberry C. Relationship between in vitro susceptibility test results for chloramphenicol and production of chloramphenicol acetyltransferase by Haemophilus influenzae, Streptococcus pneumoniae, and Aerococcus species. J Clin Microbiol. 1988 Nov;26(11):2387–2390. doi: 10.1128/jcm.26.11.2387-2390.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shanholtzer C. J., Peterson L. R. False susceptible penicillin G MICs for Streptococcus pneumoniae with a commercial microdilution system. Am J Clin Pathol. 1986 May;85(5):626–629. doi: 10.1093/ajcp/85.5.626. [DOI] [PubMed] [Google Scholar]
  13. Simberkoff M. S., Lukaszewski M., Cross A., Al-Ibrahim M., Baltch A. L., Smith R. P., Geiseler P. J., Nadler J., Richmond A. S. Antibiotic-resistant isolates of Streptococcus pneumoniae from clinical specimens: a cluster of serotype 19A organisms in Brooklyn, New York. J Infect Dis. 1986 Jan;153(1):78–82. doi: 10.1093/infdis/153.1.78. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES