Abstract
The UniScept API system was evaluated for agreement of visual versus automated readings of both its identification panels and its antimicrobial susceptibility panels. The biochemical responses of 340 oxidase-negative and oxidase-positive fermentative bacterial cultures were read both visually and automatically in the UniScept API 20E system. Automated and visual readings agreed with 99.3% of the biochemicals. Of the 45 tests that disagreed, the tests for indole and citrate were most often in disagreement. A total of 470 fermentative and nonfermentative cultures were used in the UniScept MIC system to compare visual and automated readings of susceptibility results with 17 antimicrobial agents. Agreement within +/- 1 dilution occurred with 94.1% of the enteric fermenters and with 91.7% of the other cultures. Comparison of visual and automated readings resulted in very major discrepancies in 0.95% of the readings, with the largest percentage of discrepancies associated with glucose nonfermenters (1.8%). It was felt that an automated reading is an acceptable alternative to a visual reading of the biochemicals but that 0.95% was just within the acceptable range of the 1% allowable very major discrepancies in the automated reading of susceptibilities.
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- D'Amato R. F., Isenberg H. D., McKinley G. A., Baron E. J., Tepper R., Shulman M. Novel application of video image processing to biochemical and antimicrobial susceptibility testing. J Clin Microbiol. 1988 Aug;26(8):1492–1495. doi: 10.1128/jcm.26.8.1492-1495.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davis J. R., Stager C. E., Wende R. D., Qadri S. M. Clinical laboratory evaluation of the automicrobic system Enterobacteriaceae biochemical card. J Clin Microbiol. 1981 Oct;14(4):370–375. doi: 10.1128/jcm.14.4.370-375.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matthews H. W., Baker C. N., Thornsberry C. Relationship between in vitro susceptibility test results for chloramphenicol and production of chloramphenicol acetyltransferase by Haemophilus influenzae, Streptococcus pneumoniae, and Aerococcus species. J Clin Microbiol. 1988 Nov;26(11):2387–2390. doi: 10.1128/jcm.26.11.2387-2390.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCracken A. W., Martin W. J., McCarthy L. R., Schwab D. A., Cooper B. H., Helgeson N. G., Prowant S., Robson J. Evaluation of the MS-2 system for rapid identification of Enterobacteriaceae. J Clin Microbiol. 1980 Nov;12(5):684–689. doi: 10.1128/jcm.12.5.684-689.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rhoden D. L., Smith P. B., Baker C. N., Schable B. autoSCAN-4 system for identification of gram-negative bacilli. J Clin Microbiol. 1985 Dec;22(6):915–918. doi: 10.1128/jcm.22.6.915-918.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Staneck J. L., Vincelette J., Lamothe F., Polk E. A. Evaluation of the Sensititre system for identification of Enterobacteriaceae. J Clin Microbiol. 1983 Apr;17(4):647–654. doi: 10.1128/jcm.17.4.647-654.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wenger A., Tissières L. J., Praplan F. R., Kamm W. R., Bille J. Cobas-Bact system for identification of members of the family Enterobacteriaceae in 4 h 20 min. J Clin Microbiol. 1987 Jan;25(1):61–66. doi: 10.1128/jcm.25.1.61-66.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woolfrey B. F., Lally R. T., Quall C. O. Evaluation of the AutoSCAN-3 and Sceptor systems for Enterobacteriaceae identification. J Clin Microbiol. 1983 May;17(5):807–813. doi: 10.1128/jcm.17.5.807-813.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]