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. 1990 Mar;28(3):480–483. doi: 10.1128/jcm.28.3.480-483.1990

Comparison of directigen RSV with viral isolation and direct immunofluorescence for the identification of respiratory syncytial virus.

J L Waner 1, N J Whitehurst 1, S J Todd 1, H Shalaby 1, L V Wall 1
PMCID: PMC269648  PMID: 2182668

Abstract

An enzyme immunoassay membrane test (Directigen RSV) for the detection of respiratory syncytial virus in clinical specimens was compared prospectively with isolation in cell culture and direct immunofluorescence (IF). A total of 315 nasopharyngeal wash specimens from pediatric patients were examined. Directigen RSV was 86.1% sensitive and 91.3% specific for specimens positive by isolation in cell culture and/or IF, with 88.6% agreement. The false-positive rate was 16%; 2 of 20 specimens giving false-positive reactions by Directigen RSV were true-positives by blocking assay. Twenty-seven specimens (8.5%) whose results were initially uninterpretable by Directigen RSV due to filtration difficulties were diluted and upon retesting produced acceptable results. Sixty-three viral isolates and/or IF identifications of virus antigens representing seven virus groups other than respiratory syncytial virus were also found; cross-reactions between Directigen RSV and other viruses were not observed. Directigen RSV will be useful as an immediate procedure and in facilities lacking a comprehensive virology laboratory.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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