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. 1990 Mar;28(3):573–579. doi: 10.1128/jcm.28.3.573-579.1990

Detection of immunoglobulins G and M to rubella virus by time-resolved immunofluorometry.

P Shankaran 1, E Reichstein 1, M J Khosravi 1, E P Diamandis 1
PMCID: PMC269664  PMID: 2182672

Abstract

We describe new methods for the detection of immunoglobulin G (IgG) and IgM rubella-specific antibodies in serum. The IgG assay was based on a solid-phase rubella antigen immobilization approach, and the IgM assay was based on the IgM capture assay principle. Both assays used biotinylated antibodies (anti-human IgG and antirubella monoclonal antibody, respectively). The tracer system was based on streptavidin labeled with a fluorescent europium chelate. The final measurements were done by using time-resolved fluorescence. Both assays were thoroughly evaluated with clinical samples and compared successfully with established techniques. We anticipate that these assays are suitable for routine clinical use.

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Selected References

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