Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Mar;28(3):633–636. doi: 10.1128/jcm.28.3.633-636.1990

Porcine rotaviruses antigenically related to human rotavirus serotypes 1 and 2.

R B Bellinzoni 1, N M Mattion 1, D O Matson 1, J Blackhall 1, J L La Torre 1, E A Scodeller 1, S Urasawa 1, K Taniguchi 1, M K Estes 1
PMCID: PMC269683  PMID: 2157739

Abstract

Fecal samples from rotavirus-infected piglets were characterized by a serotyping enzyme-linked immunosorbent assay (ELISA) by using monoclonal antibodies (MAbs) specific to human serotypes 1, 2, 3, and 4 (D. O. Matson, M. K. Estes, J. W. Burns, H. B. Greenberg, K. Taniguchi, and S. Urasawa, submitted for publication). Rotavirus in 19 of 25 specimens tested from two herds of pigs from Buenos Aires province, Argentina, were classified antigenically as follows: one serotype 1, four serotype 2, two serotype 3, and no serotype 4. Six specimens reacted with both serotype 1 and 2 MAbs, and viruses in six specimens probably belonged to other serotypes because they reacted only with a VP7 common epitope MAb. Two porcine rotavirus fecal samples found to contain both serotype 1 and 2 viruses by the MAb-based test and one found to contain a serotype 2 virus were grown in tissue culture. When plaque-purified preparations of these tissue culture-adapted viruses were analyzed in the serotyping ELISA, the C60 and C86 preparations reacted only as serotype 1 viruses, indicating that the original fecal samples, which showed multiple VP7 reactivities, were heterogeneous and apparently contained two types of viruses. Testing of plaque-purified C134 virus confirmed its serotype 2 reactivity. The MAb-based serotype designations of these viruses also were confirmed by using a neutralization immunoperoxidase focus reduction assay. This is the first report of the occurrence of serotype 1 and 2 rotaviruses in animals. The MAbs originally developed to serotype human rotaviruses can be utilized to type animal rotaviruses.

Full text

PDF
633

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albert M. J., Unicomb L. E., Tzipori S. R., Bishop R. F. Isolation and serotyping of animal rotaviruses and antigenic comparison with human rotaviruses. Brief report. Arch Virol. 1987;93(1-2):123–130. doi: 10.1007/BF01313898. [DOI] [PubMed] [Google Scholar]
  2. Bellinzoni R. C., Blackhall J. O., Mattion N. M., Estes M. K., Snodgrass D. R., LaTorre J. L., Scodeller E. A. Serological characterization of bovine rotaviruses isolated from dairy and beef herds in Argentina. J Clin Microbiol. 1989 Nov;27(11):2619–2623. doi: 10.1128/jcm.27.11.2619-2623.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coulson B. S., Unicomb L. E., Pitson G. A., Bishop R. F. Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses. J Clin Microbiol. 1987 Mar;25(3):509–515. doi: 10.1128/jcm.25.3.509-515.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dimitrov D. H., Estes M. K., Rangelova S. M., Shindarov L. M., Melnick J. L., Graham D. Y. Detection of antigenically distinct rotaviruses from infants. Infect Immun. 1983 Aug;41(2):523–526. doi: 10.1128/iai.41.2.523-526.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Estes M. K., Cohen J. Rotavirus gene structure and function. Microbiol Rev. 1989 Dec;53(4):410–449. doi: 10.1128/mr.53.4.410-449.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Flewett T. H., Woode G. N. The rotaviruses. Arch Virol. 1978;57(1):1–23. doi: 10.1007/BF01315633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gerna G., Sarasini A., di Matteo A., Parea M., Orsolini P., Battaglia M. Identification of two subtypes of serotype 4 human rotavirus by using VP7-specific neutralizing monoclonal antibodies. J Clin Microbiol. 1988 Jul;26(7):1388–1392. doi: 10.1128/jcm.26.7.1388-1392.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Green K. Y., Hoshino Y., Ikegami N. Sequence analysis of the gene encoding the serotype-specific glycoprotein (VP7) of two new human rotavirus serotypes. Virology. 1989 Feb;168(2):429–433. doi: 10.1016/0042-6822(89)90289-4. [DOI] [PubMed] [Google Scholar]
  9. Green K. Y., Sears J. F., Taniguchi K., Midthun K., Hoshino Y., Gorziglia M., Nishikawa K., Urasawa S., Kapikian A. Z., Chanock R. M. Prediction of human rotavirus serotype by nucleotide sequence analysis of the VP7 protein gene. J Virol. 1988 May;62(5):1819–1823. doi: 10.1128/jvi.62.5.1819-1823.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hoshino Y., Sereno M. M., Midthun K., Flores J., Kapikian A. Z., Chanock R. M. Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8701–8704. doi: 10.1073/pnas.82.24.8701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hoshino Y., Wyatt R. G., Greenberg H. B., Flores J., Kapikian A. Z. Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization. J Infect Dis. 1984 May;149(5):694–702. doi: 10.1093/infdis/149.5.694. [DOI] [PubMed] [Google Scholar]
  12. Matsui S. M., Mackow E. R., Greenberg H. B. Molecular determinant of rotavirus neutralization and protection. Adv Virus Res. 1989;36:181–214. doi: 10.1016/s0065-3527(08)60585-0. [DOI] [PubMed] [Google Scholar]
  13. Mattion N. M., Bellinzoni R. C., Blackhall J. O., La Torre J. L., Scodeller E. A. Antigenic characterization of swine rotaviruses in Argentina. J Clin Microbiol. 1989 Apr;27(4):795–798. doi: 10.1128/jcm.27.4.795-798.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nagesha H. S., Holmes I. H. New porcine rotavirus serotype antigenically related to human rotavirus serotype 3. J Clin Microbiol. 1988 Feb;26(2):171–174. doi: 10.1128/jcm.26.2.171-174.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Offit P. A., Blavat G. Identification of the two rotavirus genes determining neutralization specificities. J Virol. 1986 Jan;57(1):376–378. doi: 10.1128/jvi.57.1.376-378.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ojeh C. K., Snodgrass D. R., Herring A. J. Evidence for serotypic variation among bovine rotaviruses. Arch Virol. 1984;79(3-4):161–171. doi: 10.1007/BF01310809. [DOI] [PubMed] [Google Scholar]
  17. Pedley S., Bridger J. C., Brown J. F., McCrae M. A. Molecular characterization of rotaviruses with distinct group antigens. J Gen Virol. 1983 Oct;64(Pt 10):2093–2101. doi: 10.1099/0022-1317-64-10-2093. [DOI] [PubMed] [Google Scholar]
  18. Pongsuwanne Y., Taniguchi K., Choonthanom M., Chiwakul M., Susansook T., Saguanwongse S., Jayavasu C., Urasawa S. Subgroup and serotype distributions of human, bovine, and porcine rotavirus in Thailand. J Clin Microbiol. 1989 Sep;27(9):1956–1960. doi: 10.1128/jcm.27.9.1956-1960.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shaw R. D., Stoner-Ma D. L., Estes M. K., Greenberg H. B. Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3. J Clin Microbiol. 1985 Aug;22(2):286–291. doi: 10.1128/jcm.22.2.286-291.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sigolo de San Juan C., Bellinzoni R. C., Mattion N., La Torre J., Scodeller E. A. Incidence of group A and atypical rotaviruses in Brazilian pig herds. Res Vet Sci. 1986 Sep;41(2):270–272. [PubMed] [Google Scholar]
  21. Snodgrass D. R., Herring A. J., Campbell I., Inglis J. M., Hargreaves F. D. Comparison of atypical rotaviruses from calves, piglets, lambs and man. J Gen Virol. 1984 May;65(Pt 5):909–914. doi: 10.1099/0022-1317-65-5-909. [DOI] [PubMed] [Google Scholar]
  22. Sorrentino A., Scodeller E. A., Bellinzoni R., Muchinik G. R., La Torre J. L. Detection of an atypical rotavirus associated with diarrhoea in Chaco, Argentina. Trans R Soc Trop Med Hyg. 1986;80(1):120–122. doi: 10.1016/0035-9203(86)90209-9. [DOI] [PubMed] [Google Scholar]
  23. Taniguchi K., Urasawa S., Urasawa T. Preparation and characterization of neutralizing monoclonal antibodies with different reactivity patterns to human rotaviruses. J Gen Virol. 1985 May;66(Pt 5):1045–1053. doi: 10.1099/0022-1317-66-5-1045. [DOI] [PubMed] [Google Scholar]
  24. Urasawa S., Urasawa T., Taniguchi K., Morita Y., Sakurada N., Saeki Y., Morita O., Hasegawa S. Validity of an enzyme-linked immunosorbent assay with serotype-specific monoclonal antibodies for serotyping human rotavirus in stool specimens. Microbiol Immunol. 1988;32(7):699–708. doi: 10.1111/j.1348-0421.1988.tb01431.x. [DOI] [PubMed] [Google Scholar]
  25. Urasawa S., Urasawa T., Taniguchi K., Wakasugi F., Kobayashi N., Chiba S., Sakurada N., Morita M., Morita O., Tokieda M. Survey of human rotavirus serotypes in different locales in Japan by enzyme-linked immunosorbent assay with monoclonal antibodies. J Infect Dis. 1989 Jul;160(1):44–51. doi: 10.1093/infdis/160.1.44. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES