Abstract
As a result of the growing importance of candidemia, various techniques for the detection of Candida spp. in blood have been designed with a view to speeding up the laboratory procedure. We have performed a retrospective comparison of the efficiencies of the conventional VACUTAINER system (Becton Dickinson Vacutainer Systems) and the BACTEC NR system (Johnston Laboratories, Inc.). During a 4-year period, 88,300 blood cultures were processed, with growth of Candida species in 552. The mean times required for the detection of growth were 7.55 days with the VACUTAINER system and 4.1 days with the BACTEC NR system (P less than 0.05). During the first week of incubation, the VACUTAINER system detected 56.1% of the candidemias and the BACTEC NR detected 93.6% (P less than 0.05). Use of the BACTEC NR therefore permits reduction of the incubation period from the previously established 4 weeks to a more convenient 7 to 14 days.
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- Bille J., Edson R. S., Roberts G. D. Clinical evaluation of the lysis-centrifugation blood culture system for the detection of fungemia and comparison with a conventional biphasic broth blood culture system. J Clin Microbiol. 1984 Feb;19(2):126–128. doi: 10.1128/jcm.19.2.126-128.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bille J., Roberts G. D., Washington J. A., 2nd Retrospective comparison of three blood culture media for the recovery of yeasts from clinical specimens. Eur J Clin Microbiol. 1983 Feb;2(1):22–25. doi: 10.1007/BF02019918. [DOI] [PubMed] [Google Scholar]
- Bille J., Stockman L., Roberts G. D. Detection of yeasts and filamentous fungi in blood cultures during a 10-year period (1972 to 1981). J Clin Microbiol. 1982 Nov;16(5):968–970. doi: 10.1128/jcm.16.5.968-970.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bille J., Stockman L., Roberts G. D., Horstmeier C. D., Ilstrup D. M. Evaluation of a lysis-centrifugation system for recovery of yeasts and filamentous fungi from blood. J Clin Microbiol. 1983 Sep;18(3):469–471. doi: 10.1128/jcm.18.3.469-471.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brannon P., Kiehn T. E. Large-scale clinical comparison of the lysis-centrifugation and radiometric systems for blood culture. J Clin Microbiol. 1985 Dec;22(6):951–954. doi: 10.1128/jcm.22.6.951-954.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Braunstein H., Tomasulo M. A quantitative study of the growth of Candida albicans in vented and unvented blood-culture bottles. Am J Clin Pathol. 1976 Jul;66(1):87–90. doi: 10.1093/ajcp/66.1.87. [DOI] [PubMed] [Google Scholar]
- Caplan L. M., Merz W. G. Evaluation of two commercially prepared biphasic media for recovery of fungi from blood. J Clin Microbiol. 1978 Oct;8(4):469–470. doi: 10.1128/jcm.8.4.469-470.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Courcol R. J., Fruchart A., Roussel-Delvallez M., Martin G. R. Routine evaluation of the nonradiometric BACTEC NR 660 system. J Clin Microbiol. 1986 Jul;24(1):26–29. doi: 10.1128/jcm.24.1.26-29.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cross A. J., Haworth E., Spencer R. C. A re-evaluation of the pour plate blood culture method for the detection of Candida and other septicaemias. J Hosp Infect. 1986 Jan;7(1):74–77. doi: 10.1016/0195-6701(86)90029-0. [DOI] [PubMed] [Google Scholar]
- Dorn G. L., Land G. A., Wilson G. E. Improved blood culture technique based on centrifugation: clinical evaluation. J Clin Microbiol. 1979 Mar;9(3):391–396. doi: 10.1128/jcm.9.3.391-396.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gantz N. M., Medeiros A. A., Swain J. L., O'Brien T. F. Vacuum blood-culture bottles inhibiting growth of Candida and fostering growth of Bacteroides. Lancet. 1974 Nov 16;2(7890):1174–1176. doi: 10.1016/s0140-6736(74)90813-7. [DOI] [PubMed] [Google Scholar]
- Hopfer R. L., Orengo A., Chesnut S., Wenglar M. Radiometric detection of yeasts in blood cultures of cancer patients. J Clin Microbiol. 1980 Sep;12(3):329–331. doi: 10.1128/jcm.12.3.329-331.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Horn R., Wong B., Kiehn T. E., Armstrong D. Fungemia in a cancer hospital: changing frequency, earlier onset, and results of therapy. Rev Infect Dis. 1985 Sep-Oct;7(5):646–655. doi: 10.1093/clinids/7.5.646. [DOI] [PubMed] [Google Scholar]
- Jungkind D., Millan J., Allen S., Dyke J., Hill E. Clinical comparison of a new automated infrared blood culture system with the BACTEC 460 system. J Clin Microbiol. 1986 Feb;23(2):262–266. doi: 10.1128/jcm.23.2.262-266.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kiehn T. E., Capitolo C., Mayo J. B., Armstrong D. Comparative recovery of fungi from biphasic and conventional blood culture media. J Clin Microbiol. 1981 Dec;14(6):681–683. doi: 10.1128/jcm.14.6.681-683.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kiehn T. E., Wong B., Edwards F. F., Armstrong D. Comparative recovery of bacteria and yeasts from lysis-centrifugation and a conventional blood culture system. J Clin Microbiol. 1983 Aug;18(2):300–304. doi: 10.1128/jcm.18.2.300-304.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Komorowski R. A., Farmer S. G. Rapid detection of candidemia. Am J Clin Pathol. 1973 Jan;59(1):56–61. doi: 10.1093/ajcp/59.1.56. [DOI] [PubMed] [Google Scholar]
- Land G. A., Dorn G. L., Fleming W. H., 3rd, Beadles T. A., Foxworth J. H. Isolation and rapid identification of yeasts from compromised hosts. Mycopathologia. 1978 Dec 18;65(1-3):123–131. doi: 10.1007/BF00447183. [DOI] [PubMed] [Google Scholar]
- Myerowitz R. L., Pazin G. J., Allen C. M. Disseminated candidiasis. Changes in incidence, underlying diseases, and pathology. Am J Clin Pathol. 1977 Jul;68(1):29–38. doi: 10.1093/ajcp/68.1.29. [DOI] [PubMed] [Google Scholar]
- Prevost E., Bannister E. Detection of yeast septicemia by biphasic and radiometric methods. J Clin Microbiol. 1981 Apr;13(4):655–660. doi: 10.1128/jcm.13.4.655-660.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reingold A. L., Lu X. D., Plikaytis B. D., Ajello L. Systemic mycoses in the United States, 1980-1982. J Med Vet Mycol. 1986 Dec;24(6):433–436. [PubMed] [Google Scholar]
- Reller L. B. Recent and innovative methods for detection of bacteremia and fungemia. Am J Med. 1983 Jul 28;75(1B):26–30. doi: 10.1016/0002-9343(83)90069-4. [DOI] [PubMed] [Google Scholar]
- Renner E. D., Gatheridge L. A., Washington J. A., 2nd Evaluation of radiometric system for detecting bacteremia. Appl Microbiol. 1973 Sep;26(3):368–372. doi: 10.1128/am.26.3.368-372.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts G. D., Horstmeier C., Hall M., Washington J. A., 2nd Recovery of yeast from vented blood culture bottles. J Clin Microbiol. 1975 Jul;2(1):18–20. doi: 10.1128/jcm.2.1.18-20.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts G. D., Washington J. A., 2nd Detection of fungi in blood cultures. J Clin Microbiol. 1975 Mar;1(3):309–310. doi: 10.1128/jcm.1.3.309-310.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]