Abstract
The Sceptor system (Becton Dickinson Diagnostic Instrument Systems, Towson Md.) was assessed for its ability to identify veterinary clinical isolates. A total of 605 bacteria, including 315 isolates of the family Enterobacteriaceae, 191 gram-negative nonenteric bacteria, and 99 gram-positive bacteria, were tested. Overall, 534 (88.3%) were correctly identified, 28 (4.6%) were not identified, 12 (2.0%) were incorrectly identified at the genus levels, and 32 (5.3%) were incorrectly identified at the species level. The Sceptor system correctly identified 292 (92.7%) isolates of Enterobacteriaceae, 165 (86.4%) gram-negative nonenteric bacteria, and 77 (77.8%) gram-positive bacteria. One hundred thirty organisms not contained in the data base were tested with the Sceptor system to assess the possibility of expanding the data base. The Sceptor system was an acceptable method for the identification of isolates of Enterobacteriaceae but not gram-negative nonenteric and gram-positive bacteria of animal origin. Development of a veterinary isolate-specific data base would improve the utility of the Sceptor system in veterinary diagnostic bacteriology.
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Selected References
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- Collins M. T., Swanson E. C. Use of the API 20E system to identify non-Enterobacteriaceae from veterinary medical sources. Am J Vet Res. 1981 Jul;42(7):1269–1273. [PubMed] [Google Scholar]
- Cornaglia G., Dainelli B., Berlutti F., Thaller M. C. Commercial identification systems often fail to identify Providencia stuartii. J Clin Microbiol. 1988 Feb;26(2):323–327. doi: 10.1128/jcm.26.2.323-327.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Devenish J. A., Barnum D. A. Evaluation of the API 20E system for the identification of gram-negative nonfermenters from animal origin. Can J Comp Med. 1982 Jan;46(1):80–84. [PMC free article] [PubMed] [Google Scholar]
- Gross A. J., Huppert M. A test of significance for determining if one new testing procedure is better than another relative to a standard procedure. J Chronic Dis. 1971 Aug;24(5):285–288. doi: 10.1016/0021-9681(71)90118-4. [DOI] [PubMed] [Google Scholar]
- Hubbard G. B. Aeromonas hydrophila infection in Xenopus laevis. Lab Anim Sci. 1981 Jun;31(3):297–300. [PubMed] [Google Scholar]
- Jones R. L., Adney W. S., Davis M. A., Vonbyren H., Thompson G. Evaluation of Quantum II microbiology system for identification of gram-negative bacteria of veterinary origin. J Clin Microbiol. 1987 Nov;25(11):2071–2074. doi: 10.1128/jcm.25.11.2071-2074.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaper J. B., Lockman H., Colwell R. R., Joseph S. W. Aeromonas hydrophila: ecology and toxigenicity of isolates from an estuary. J Appl Bacteriol. 1981 Apr;50(2):359–377. doi: 10.1111/j.1365-2672.1981.tb00900.x. [DOI] [PubMed] [Google Scholar]
- Motyl M. R., McKinley G., Janda J. M. In vitro susceptibilities of Aeromonas hydrophila, Aeromonas sobria, and Aeromonas caviae to 22 antimicrobial agents. Antimicrob Agents Chemother. 1985 Jul;28(1):151–153. doi: 10.1128/aac.28.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schiemann D. A. Yersinia enterocolitica: observations on some growth characteristics and response to selective agents. Can J Microbiol. 1980 Oct;26(10):1232–1240. doi: 10.1139/m80-205. [DOI] [PubMed] [Google Scholar]
- Swanson E. C., Collins M. T. Use of the API 20E system to identify veterinary Enterobacteriaceae. J Clin Microbiol. 1980 Jul;12(1):10–14. doi: 10.1128/jcm.12.1.10-14.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watts J. L. Evaluation of the Minitek Gram-Positive Set for identification of streptococci isolated from bovine mammary glands. J Clin Microbiol. 1989 May;27(5):1008–1010. doi: 10.1128/jcm.27.5.1008-1010.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watts J. L., Owens W. E., Nickerson S. C. Identification of staphylococci from bovine udders: evaluation of the API 20GP system. Can J Microbiol. 1986 Apr;32(4):359–361. doi: 10.1139/m86-069. [DOI] [PubMed] [Google Scholar]
- Woolfrey B. F., Lally R. T., Quall C. O. Evaluation of the AutoSCAN-3 and Sceptor systems for Enterobacteriaceae identification. J Clin Microbiol. 1983 May;17(5):807–813. doi: 10.1128/jcm.17.5.807-813.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
