Abstract
Two systems, inoculation of bovine endothelial cells and of embryonated chicken eggs, were compared for detection of bluetongue virus (BTV) in blood specimens from experimentally inoculated sheep. For all BTV serotypes tested, embryonated chicken eggs detected longer periods of viremia than did bovine endothelial cells, primarily by detecting BTV in samples containing lower virus concentrations.
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Selected References
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- Foster N. M., Leudke A. J. Direct assay for bluetongue virus by intravascular inoculation of embryonating chicken eggs. Am J Vet Res. 1968 Mar;29(3):749–753. [PubMed] [Google Scholar]
- Foster N. M., Luedke A. J., Metcalf H. E. Bluetongue in sheep and cattle: efficacy of embryonating chicken eggs in viral isolations. Am J Vet Res. 1972 Jan;33(1):77–81. [PubMed] [Google Scholar]
- Gibbs E. P., Greiner E. C., Taylor W. P., Barber T. L., House J. A., Pearson J. E. Isolation of bluetongue virus serotype 2 from cattle in Florida: serotype of bluetongue virus hitherto unrecognized in the Western Hemisphere. Am J Vet Res. 1983 Dec;44(12):2226–2228. [PubMed] [Google Scholar]
- Goldsmit L., Barzilai E., Tadmor A. The comparative sensitivity of sheep and chicken embryos to bluetongue virus and observations on viraemia in experimentally infected sheep. Aust Vet J. 1975 Apr;51(4):190–196. doi: 10.1111/j.1751-0813.1975.tb00053.x. [DOI] [PubMed] [Google Scholar]
- Jochim M. M. An overview of diagnostics for bluetongue. Prog Clin Biol Res. 1985;178:423–433. [PubMed] [Google Scholar]
- Luedke A. J. Bluetongue in sheep: viral assay and viremia. Am J Vet Res. 1969 Apr;30(4):499–509. [PubMed] [Google Scholar]
- McPhee D. A., Parsonson I. M., Della-Porta A. J. Comparative studies on the growth of Australian bluetongue virus serotypes in continuous cell lines and embryonated chicken eggs. Vet Microbiol. 1982 Nov;7(5):401–410. doi: 10.1016/0378-1135(82)90056-6. [DOI] [PubMed] [Google Scholar]
- Metcalf H. E., Lomme J., Beal V. C., Jr Estimate of incidence and direct economic losses due to bluetongue in Mississippi cattle during 1979. Proc Annu Meet U S Anim Health Assoc. 1980;84:186–202. [PubMed] [Google Scholar]
- Sawyer M. M., Osburn B. L. Isolation of bluetongue vaccine virus from infected sheep by direct inoculation onto tissue culture. Cornell Vet. 1977 Jan;67(1):65–71. [PubMed] [Google Scholar]
- Wechsler S. J., Austin K. J., Wilson W. C. Limits of detection of bluetongue virus with different assay systems. J Vet Diagn Invest. 1990 Apr;2(2):103–106. doi: 10.1177/104063879000200203. [DOI] [PubMed] [Google Scholar]
- Wechsler S. J., McHolland L. E. Susceptibilities of 14 cell lines to bluetongue virus infection. J Clin Microbiol. 1988 Nov;26(11):2324–2327. doi: 10.1128/jcm.26.11.2324-2327.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]