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. 1991 Feb;29(2):227–230. doi: 10.1128/jcm.29.2.227-230.1991

Amplification of repetitive DNA for the specific detection of Naegleria fowleri.

G L McLaughlin 1, M H Vodkin 1, H W Huizinga 1
PMCID: PMC269744  PMID: 2007628

Abstract

By using hybridization at low C0t values, a genomic library on Naegleria fowleri was screened for clones containing repetitive DNA. Partial sequence information from a repetitive clone, Nf9, showed sequence homologies with the mitochondrial ATPase 6 subunit from yeasts and other organisms. Synthetic DNA primers were selected and tested in amplification reactions. Nonstringent hybridization conditions were defined which allowed amplification of N. fowleri DNA and reduced amplification of DNA from nonpathogenic Naegleria species. Stringent conditions were selected which allowed detection only of N. fowleri. Identity of the amplified DNA was confirmed by using internal restriction sites and an internal primer. In a blind study, tissue from mice experimentally infected with N. fowleri was specifically detected by using stringent hybridization conditions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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