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. 1991 Feb;29(2):310–314. doi: 10.1128/jcm.29.2.310-314.1991

Differentiation of Acanthamoeba strains from infected corneas and the environment by using restriction endonuclease digestion of whole-cell DNA.

S Kilvington 1, J R Beeching 1, D G White 1
PMCID: PMC269759  PMID: 1672534

Abstract

Restriction endonuclease digestion of Acanthamoeba whole-cell DNA was used to study the relationship between 33 morphologically identical strains from keratitis cases (30 strains), contact lens storage containers (2 strains), and soil (1 strain). Samples digested with BglII, EcoRI, or HindIII and separated by agarose gel electrophoresis contained detectable mitochondrial DNA restriction fragment length polymorphisms (RFLPs). By comparing RFLPs, the strains could be assigned to seven multiple-strain and three single-strain groups. The largest of these contained nine strains, eight of which were isolated in keratitis cases in various locations worldwide and may indicate a group particularly associated with keratitis. Restriction endonuclease analysis of whole-cell DNA is proposed as a valuable technique for detecting mitochondrial DNA RFLPs in the differentiation of morphologically identical Acanthamoeba strains and may therefore be useful in resolving the complex taxonomy of the genus, which has hitherto been founded on subjective morphological criteria.

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Selected References

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  1. Aufy S., Kilvington S., Mann P. G., Warhurst D. C. Improved selective isolation of Naegleria fowleri from the environment. Trans R Soc Trop Med Hyg. 1986;80(2):350–351. doi: 10.1016/0035-9203(86)90060-x. [DOI] [PubMed] [Google Scholar]
  2. Badenoch P. R., Johnson A. M., Christy P. E., Coster D. J. Pathogenicity of Acanthamoeba and a Corynebacterium in the rat cornea. Arch Ophthalmol. 1990 Jan;108(1):107–112. doi: 10.1001/archopht.1990.01070030113040. [DOI] [PubMed] [Google Scholar]
  3. Bogler S. A., Zarley C. D., Burianek L. L., Fuerst P. A., Byers T. J. Interstrain mitochondrial DNA polymorphism detected in Acanthamoeba by restriction endonuclease analysis. Mol Biochem Parasitol. 1983 Jun;8(2):145–163. doi: 10.1016/0166-6851(83)90006-3. [DOI] [PubMed] [Google Scholar]
  4. Casemore D. P. Sensitivity of Hartmannella (Acanthamoeba) to 5-fluorocytosine, hydroxystilbamidine, and other substances. J Clin Pathol. 1970 Nov;23(8):649–652. doi: 10.1136/jcp.23.8.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cohen E. J., Parlato C. J., Arentsen J. J., Genvert G. I., Eagle R. C., Jr, Wieland M. R., Laibson P. R. Medical and surgical treatment of Acanthamoeba keratitis. Am J Ophthalmol. 1987 May 15;103(5):615–625. doi: 10.1016/s0002-9394(14)74320-x. [DOI] [PubMed] [Google Scholar]
  6. De Jonckheere J. F. Growth characteristics, cytopathic effect in cell culture, and virulence in mice of 36 type strains belonging to 19 different Acanthamoeba spp. Appl Environ Microbiol. 1980 Apr;39(4):681–685. doi: 10.1128/aem.39.4.681-685.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jones D. B. Acanthamoeba--the ultimate opportunist? Am J Ophthalmol. 1986 Oct 15;102(4):527–530. doi: 10.1016/0002-9394(86)90085-1. [DOI] [PubMed] [Google Scholar]
  8. Jones D. B., Visvesvara G. S., Robinson N. M. Acanthamoeba polyphaga keratitis and Acenthamoeba uveitis associated with fatal meningoencephalitis. Trans Ophthalmol Soc U K. 1975 Jul;95(2):221–232. [PubMed] [Google Scholar]
  9. Kilvington S., Larkin D. F., White D. G., Beeching J. R. Laboratory investigation of Acanthamoeba keratitis. J Clin Microbiol. 1990 Dec;28(12):2722–2725. doi: 10.1128/jcm.28.12.2722-2725.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kingston D., Warhurst D. C. Isolation of amoebae from the air. J Med Microbiol. 1969 Feb;2(1):27–36. doi: 10.1099/00222615-2-1-27. [DOI] [PubMed] [Google Scholar]
  11. Ludwig I. H., Meisler D. M., Rutherford I., Bican F. E., Langston R. H., Visvesvara G. S. Susceptibility of Acanthamoeba to soft contact lens disinfection systems. Invest Ophthalmol Vis Sci. 1986 Apr;27(4):626–628. [PubMed] [Google Scholar]
  12. McLaughlin G. L., Brandt F. H., Visvesvara G. S. Restriction fragment length polymorphisms of the DNA of selected Naegleria and Acanthamoeba amebae. J Clin Microbiol. 1988 Sep;26(9):1655–1658. doi: 10.1128/jcm.26.9.1655-1658.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moore M. B., McCulley J. P., Newton C., Cobo L. M., Foulks G. N., O'Day D. M., Johns K. J., Driebe W. T., Wilson L. A., Epstein R. J. Acanthamoeba keratitis. A growing problem in soft and hard contact lens wearers. Ophthalmology. 1987 Dec;94(12):1654–1661. [PubMed] [Google Scholar]
  14. Nagington J., Richards J. E. Chemotherapeutic compounds and Acanthamoebae from eye infections. J Clin Pathol. 1976 Jul;29(7):648–651. doi: 10.1136/jcp.29.7.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Naginton J., Watson P. G., Playfair T. J., McGill J., Jones B. R., Steele A. D. Amoebic infection of the eye. Lancet. 1974 Dec 28;2(7896):1537–1540. doi: 10.1016/s0140-6736(74)90285-2. [DOI] [PubMed] [Google Scholar]
  16. Nelson M., McClelland M. The effect of site-specific methylation on restriction-modification enzymes. Nucleic Acids Res. 1987;15 (Suppl):r219–r230. doi: 10.1093/nar/15.suppl.r219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stehr-Green J. K., Bailey T. M., Brandt F. H., Carr J. H., Bond W. W., Visvesvara G. S. Acanthamoeba keratitis in soft contact lens wearers. A case-control study. JAMA. 1987 Jul 3;258(1):57–60. [PubMed] [Google Scholar]
  18. Stehr-Green J. K., Bailey T. M., Visvesvara G. S. The epidemiology of Acanthamoeba keratitis in the United States. Am J Ophthalmol. 1989 Apr 15;107(4):331–336. doi: 10.1016/0002-9394(89)90654-5. [DOI] [PubMed] [Google Scholar]
  19. Stevens A. R., O'Dell W. D. In vitro growth and virulence of Acanthamoeba. J Parasitol. 1974 Oct;60(5):884–885. [PubMed] [Google Scholar]
  20. Visvesvara G. S., Jones D. B., Robinson N. M. Isolation, identification, and biological characterization of Acanthamoeba polyphaga from a human eye. Am J Trop Med Hyg. 1975 Sep;24(5):784–790. doi: 10.4269/ajtmh.1975.24.784. [DOI] [PubMed] [Google Scholar]
  21. Warhurst D. C. Pathogenic free-living amoebae. Parasitol Today. 1985 Jul;1(1):24–28. doi: 10.1016/0169-4758(85)90102-4. [DOI] [PubMed] [Google Scholar]
  22. Wright P., Warhurst D., Jones B. R. Acanthamoeba keratitis successfully treated medically. Br J Ophthalmol. 1985 Oct;69(10):778–782. doi: 10.1136/bjo.69.10.778. [DOI] [PMC free article] [PubMed] [Google Scholar]

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