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. 1991 Mar;29(3):431–435. doi: 10.1128/jcm.29.3.431-435.1991

New oligopeptide immunoglobulin G test for human parvovirus B19 antibodies.

T F Schwarz 1, S Modrow 1, B Hottenträger 1, B Höflacher 1, G Jäger 1, W Scharti 1, R Sumazakl 1, H Wolf 1, J Middeldorp 1, M Roggendorf 1, et al.
PMCID: PMC269794  PMID: 1645365

Abstract

A new, highly sensitive and specific enzyme immunoassay using oligopeptides as antigen (enzyme-linked immunosorbent assay [ELISA] B19-OP) for detecting parvovirus B19-specific immunoglobulin G (IgG) was established. As antigens, B19-specific oligopeptides of 24 and 30 kDa derived from a 196-kDa fusion protein of beta-galactosidase and viral capsid protein (VPI) of B19 after CNBr cleavage and separation by high-pressure liquid chromatography were used. Of 139 serum specimens tested in parallel for anti-B19 IgG by standard ELISA using B19 particles as antigen and by ELISA B19-OP, 73 (52.5%) were positive and 63 (45.3%) were negative in both tests, and 3 (2.2%) were negative by standard ELISA but positive by ELISA B19-OP and by immunoblot. By using ELISA B19-OP, it was possible to detect anti-B19 IgG in an asymptomatic blood donor 4 weeks after acute infection, and anti-B19 IgG titers of 10(-5) could be measured in convalescent-phase sera.

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Selected References

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