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. 1991 Mar;29(3):524–532. doi: 10.1128/jcm.29.3.524-532.1991

Polymerase chain reaction analyses identify two distinct classes of Borrelia burgdorferi.

P A Rosa 1, D Hogan 1, T G Schwan 1
PMCID: PMC269812  PMID: 2037670

Abstract

We sequenced homologous chromosomal loci from several North American and European isolates of the Lyme disease spirochete Borrelia burgdorferi, as well as from the relapsing fever spirochete Borrelia hermsii. Inter- and intraspecies sequence comparisons permitted the design of B. burgdorferi-specific polymerase chain reaction primers that detected all strains tested (n = 31) from diverse geographical and biological origins. Polymerase chain reaction "typing" with other unique sets of primers subdivided B. burgdorferi isolates into two groups: all North American isolates and a few European isolates made up one group, while the majority of the European and Asian isolates made up the second group. This classification may have a clinical correlate reflected in differences between "typical" Lyme borreliosis in North America and Europe.

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Selected References

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  1. Ackermann R., Rehse-Küpper B., Gollmer E., Schmidt R. Chronic neurologic manifestations of erythema migrans borreliosis. Ann N Y Acad Sci. 1988;539:16–23. doi: 10.1111/j.1749-6632.1988.tb31834.x. [DOI] [PubMed] [Google Scholar]
  2. Asbrink E., Hovmark A. Early and late cutaneous manifestations in Ixodes-borne borreliosis (erythema migrans borreliosis, Lyme borreliosis). Ann N Y Acad Sci. 1988;539:4–15. doi: 10.1111/j.1749-6632.1988.tb31833.x. [DOI] [PubMed] [Google Scholar]
  3. Barbour A. G. Isolation and cultivation of Lyme disease spirochetes. Yale J Biol Med. 1984 Jul-Aug;57(4):521–525. [PMC free article] [PubMed] [Google Scholar]
  4. Barbour A. G. Plasmid analysis of Borrelia burgdorferi, the Lyme disease agent. J Clin Microbiol. 1988 Mar;26(3):475–478. doi: 10.1128/jcm.26.3.475-478.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Barbour A. G., Tessier S. L., Hayes S. F. Variation in a major surface protein of Lyme disease spirochetes. Infect Immun. 1984 Jul;45(1):94–100. doi: 10.1128/iai.45.1.94-100.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Benach J. L., Coleman J. L., Skinner R. A., Bosler E. M. Adult Ixodes dammini on rabbits: a hypothesis for the development and transmission of Borrelia burgdorferi. J Infect Dis. 1987 Jun;155(6):1300–1306. doi: 10.1093/infdis/155.6.1300. [DOI] [PubMed] [Google Scholar]
  7. Bergström S., Bundoc V. G., Barbour A. G. Molecular analysis of linear plasmid-encoded major surface proteins, OspA and OspB, of the Lyme disease spirochaete Borrelia burgdorferi. Mol Microbiol. 1989 Apr;3(4):479–486. doi: 10.1111/j.1365-2958.1989.tb00194.x. [DOI] [PubMed] [Google Scholar]
  8. Bundoc V. G., Barbour A. G. Clonal polymorphisms of outer membrane protein OspB of Borrelia burgdorferi. Infect Immun. 1989 Sep;57(9):2733–2741. doi: 10.1128/iai.57.9.2733-2741.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Burgdorfer W., Barbour A. G., Hayes S. F., Benach J. L., Grunwaldt E., Davis J. P. Lyme disease-a tick-borne spirochetosis? Science. 1982 Jun 18;216(4552):1317–1319. doi: 10.1126/science.7043737. [DOI] [PubMed] [Google Scholar]
  10. Burgdorfer W., Hayes S. F., Benach J. L. Development of Borrelia burgdorferi in ixodid tick vectors. Ann N Y Acad Sci. 1988;539:172–179. doi: 10.1111/j.1749-6632.1988.tb31851.x. [DOI] [PubMed] [Google Scholar]
  11. Craft J. E., Grodzicki R. L., Steere A. C. Antibody response in Lyme disease: evaluation of diagnostic tests. J Infect Dis. 1984 May;149(5):789–795. doi: 10.1093/infdis/149.5.789. [DOI] [PubMed] [Google Scholar]
  12. Dattwyler R. J., Volkman D. J., Luft B. J., Halperin J. J., Thomas J., Golightly M. G. Seronegative Lyme disease. Dissociation of specific T- and B-lymphocyte responses to Borrelia burgdorferi. N Engl J Med. 1988 Dec 1;319(22):1441–1446. doi: 10.1056/NEJM198812013192203. [DOI] [PubMed] [Google Scholar]
  13. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  14. Halperin J. J., Pass H. L., Anand A. K., Luft B. J., Volkman D. J., Dattwyler R. J. Nervous system abnormalities in Lyme disease. Ann N Y Acad Sci. 1988;539:24–34. doi: 10.1111/j.1749-6632.1988.tb31835.x. [DOI] [PubMed] [Google Scholar]
  15. Huppertz H. I., Sticht-Groh V., Schwan T. Borderline antibody response in initial stages of lymphocytic meningitis does not rule out borreliosis. Lancet. 1986 Dec 20;2(8521-22):1468–1469. doi: 10.1016/s0140-6736(86)92789-3. [DOI] [PubMed] [Google Scholar]
  16. Hyde F. W., Johnson R. C. Genetic relationship of lyme disease spirochetes to Borrelia, Treponema, and Leptospira spp. J Clin Microbiol. 1984 Aug;20(2):151–154. doi: 10.1128/jcm.20.2.151-154.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lane R. S., Burgdorfer W., Hayes S. F., Barbour A. G. Isolation of a spirochete from the soft tick, Ornithodoros coriaceus: a possible agent of epizootic bovine abortion. Science. 1985 Oct 4;230(4721):85–87. doi: 10.1126/science.3898367. [DOI] [PubMed] [Google Scholar]
  18. Magnarelli L. A., Anderson J. F., Johnson R. C. Cross-reactivity in serological tests for Lyme disease and other spirochetal infections. J Infect Dis. 1987 Jul;156(1):183–188. doi: 10.1093/infdis/156.1.183. [DOI] [PubMed] [Google Scholar]
  19. Malloy D. C., Nauman R. K., Paxton H. Detection of Borrelia burgdorferi using the polymerase chain reaction. J Clin Microbiol. 1990 Jun;28(6):1089–1093. doi: 10.1128/jcm.28.6.1089-1093.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mullis K. B., Faloona F. A. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 1987;155:335–350. doi: 10.1016/0076-6879(87)55023-6. [DOI] [PubMed] [Google Scholar]
  21. Mullis K., Faloona F., Scharf S., Saiki R., Horn G., Erlich H. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harb Symp Quant Biol. 1986;51(Pt 1):263–273. doi: 10.1101/sqb.1986.051.01.032. [DOI] [PubMed] [Google Scholar]
  22. Nielsen S. L., Young K. K., Barbour A. G. Detection of Borrelia burgdorferi DNA by the polymerase chain reaction. Mol Cell Probes. 1990 Feb;4(1):73–79. doi: 10.1016/0890-8508(90)90041-w. [DOI] [PubMed] [Google Scholar]
  23. Persing D. H., Telford S. R., 3rd, Spielman A., Barthold S. W. Detection of Borrelia burgdorferi infection in Ixodes dammini ticks with the polymerase chain reaction. J Clin Microbiol. 1990 Mar;28(3):566–572. doi: 10.1128/jcm.28.3.566-572.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Postic D., Edlinger C., Richaud C., Grimont F., Dufresne Y., Perolat P., Baranton G., Grimont P. A. Two genomic species in Borrelia burgdorferi. Res Microbiol. 1990 May;141(4):465–475. doi: 10.1016/0923-2508(90)90072-x. [DOI] [PubMed] [Google Scholar]
  25. Reik L., Steere A. C., Bartenhagen N. H., Shope R. E., Malawista S. E. Neurologic abnormalities of Lyme disease. Medicine (Baltimore) 1979 Jul;58(4):281–294. doi: 10.1097/00005792-197907000-00001. [DOI] [PubMed] [Google Scholar]
  26. Ribeiro J. M., Mather T. N., Piesman J., Spielman A. Dissemination and salivary delivery of Lyme disease spirochetes in vector ticks (Acari: Ixodidae). J Med Entomol. 1987 Mar;24(2):201–205. doi: 10.1093/jmedent/24.2.201. [DOI] [PubMed] [Google Scholar]
  27. Rosa P. A., Schwan T. G. A specific and sensitive assay for the Lyme disease spirochete Borrelia burgdorferi using the polymerase chain reaction. J Infect Dis. 1989 Dec;160(6):1018–1029. doi: 10.1093/infdis/160.6.1018. [DOI] [PubMed] [Google Scholar]
  28. Saiki R. K., Scharf S., Faloona F., Mullis K. B., Horn G. T., Erlich H. A., Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350–1354. doi: 10.1126/science.2999980. [DOI] [PubMed] [Google Scholar]
  29. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Schmid G. P., Steigerwalt A. G., Johnson S. E., Barbour A. G., Steere A. C., Robinson I. M., Brenner D. J. DNA characterization of the spirochete that causes Lyme disease. J Clin Microbiol. 1984 Aug;20(2):155–158. doi: 10.1128/jcm.20.2.155-158.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Schwan T. G., Burgdorfer W. Antigenic changes of Borrelia burgdorferi as a result of in vitro cultivation. J Infect Dis. 1987 Nov;156(5):852–853. doi: 10.1093/infdis/156.5.852-a. [DOI] [PubMed] [Google Scholar]
  32. Schwan T. G., Burgdorfer W., Garon C. F. Changes in infectivity and plasmid profile of the Lyme disease spirochete, Borrelia burgdorferi, as a result of in vitro cultivation. Infect Immun. 1988 Aug;56(8):1831–1836. doi: 10.1128/iai.56.8.1831-1836.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Schwan T. G., Simpson W. J., Schrumpf M. E., Karstens R. H. Identification of Borrelia burgdorferi and B. hermsii using DNA hybridization probes. J Clin Microbiol. 1989 Aug;27(8):1734–1738. doi: 10.1128/jcm.27.8.1734-1738.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Steere A. C., Batsford W. P., Weinberg M., Alexander J., Berger H. J., Wolfson S., Malawista S. E. Lyme carditis: cardiac abnormalities of Lyme disease. Ann Intern Med. 1980 Jul;93(1):8–16. doi: 10.7326/0003-4819-93-1-8. [DOI] [PubMed] [Google Scholar]
  35. Steere A. C., Grodzicki R. L., Kornblatt A. N., Craft J. E., Barbour A. G., Burgdorfer W., Schmid G. P., Johnson E., Malawista S. E. The spirochetal etiology of Lyme disease. N Engl J Med. 1983 Mar 31;308(13):733–740. doi: 10.1056/NEJM198303313081301. [DOI] [PubMed] [Google Scholar]
  36. Steere A. C. Lyme disease. N Engl J Med. 1989 Aug 31;321(9):586–596. doi: 10.1056/NEJM198908313210906. [DOI] [PubMed] [Google Scholar]
  37. Steere A. C., Malawista S. E., Hardin J. A., Ruddy S., Askenase W., Andiman W. A. Erythema chronicum migrans and Lyme arthritis. The enlarging clinical spectrum. Ann Intern Med. 1977 Jun;86(6):685–698. doi: 10.7326/0003-4819-86-6-685. [DOI] [PubMed] [Google Scholar]
  38. Steere A. C., Malawista S. E., Snydman D. R., Shope R. E., Andiman W. A., Ross M. R., Steele F. M. Lyme arthritis: an epidemic of oligoarticular arthritis in children and adults in three connecticut communities. Arthritis Rheum. 1977 Jan-Feb;20(1):7–17. doi: 10.1002/art.1780200102. [DOI] [PubMed] [Google Scholar]
  39. Steere A. C., Schoen R. T., Taylor E. The clinical evolution of Lyme arthritis. Ann Intern Med. 1987 Nov;107(5):725–731. doi: 10.7326/0003-4819-107-5-725. [DOI] [PubMed] [Google Scholar]
  40. Stiernstedt G., Gustafsson R., Karlsson M., Svenungsson B., Sköldenberg B. Clinical manifestations and diagnosis of neuroborreliosis. Ann N Y Acad Sci. 1988;539:46–55. doi: 10.1111/j.1749-6632.1988.tb31837.x. [DOI] [PubMed] [Google Scholar]
  41. Wallich R., Moter S. E., Simon M. M., Ebnet K., Heiberger A., Kramer M. D. The Borrelia burgdorferi flagellum-associated 41-kilodalton antigen (flagellin): molecular cloning, expression, and amplification of the gene. Infect Immun. 1990 Jun;58(6):1711–1719. doi: 10.1128/iai.58.6.1711-1719.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Wheeler C. M., Coleman J. L., Habicht G. S., Benach J. L. Adult Ixodes dammini on rabbits: development of acute inflammation in the skin and immune responses to salivary gland, midgut, and spirochetal components. J Infect Dis. 1989 Feb;159(2):265–273. doi: 10.1093/infdis/159.2.265. [DOI] [PubMed] [Google Scholar]
  43. Wilske B., Preac-Mursic V., Schierz G., Kühbeck R., Barbour A. G., Kramer M. Antigenic variability of Borrelia burgdorferi. Ann N Y Acad Sci. 1988;539:126–143. doi: 10.1111/j.1749-6632.1988.tb31846.x. [DOI] [PubMed] [Google Scholar]

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