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. 1991 Mar;29(3):560–564. doi: 10.1128/jcm.29.3.560-564.1991

Aeromonas jandaei (formerly genospecies DNA group 9 A. sobria), a new sucrose-negative species isolated from clinical specimens.

A Carnahan 1, G R Fanning 1, S W Joseph 1
PMCID: PMC269818  PMID: 2037673

Abstract

A large numerical taxonomy study conducted in 1988 of 165 mostly clinical Aeromonas strains from diverse geographic sources produced a cluster (S = 84%, SSM) of four sucrose-negative strains that included the DNA definition strain for DNA group 9 A. sobria (CDC 0787-80). These four strains, together with five additional strains received in 1989, were subjected to DNA-DNA hybridization (hydroxyapatite, 32P, 60 and 75 degrees C), and all eight strains were closely related to the ninth labeled DNA group 9 definition strain CDC 0787-80 (73 to 86% relatedness at 60 degrees C and 68 to 80% relatedness at 75 degrees C; percent divergence, 2.0 to 3.5). Type strains and DNA definition strains for all other established Aeromonas species were only 35 to 72% related (60 degrees C) to CDC 0787-80. We propose the name Aeromonas jandaei for this highly related group of nine strains, formerly known as DNA group 9 A. sobria. The type strain was designated ATCC 49568 (CDC 0787-80). The nine strains were examined at 36 degrees C and were found to be resistant to 0/129 (vibriostatic agent) and uniformly positive for oxidase, gas production from glucose, indole, lysine decarboxylase, arginine dihydrolase, o-nitrophenyl-beta-D-galactopyranoside, motility (25 degrees C), nitrate reduction, citrate utilization, hemolysis on sheep blood agar, and growth in Trypticase soy broth with no added NaCl. They all fermented D-glucose, D-mannitol, and mannose but did not ferment sucrose, cellobiose, L-arabinose, inositol, salicin, or D-sorbitol. They were uniformly negative for esculin and urea hydrolysis, elastase production, ornithine decarboxylation, and the string test. The antibiogram of A. jandaei resembled that of other aeromonads (resistance to ampicillin and cephalothin), but it differed from most other aeromonads because of resistance to single dilution of colistin and differed from clinical A. veronii biogroup sorbria (formerly A. sobria) by its nearly uniform resistance to cephalothin. The esculin-, sucrose-, and cellobiose-negative and colistin-resistant profile distinguished A. jandaei from other Aeromonas species. These A. jandaei strains were isolated from blood (two strains), wounds (two strains), diarrheal stools (four strains), and a prawn (one strain). The blood and wound isolates, in particular, suggest that there is a possible clinical significance for this species and justify identification of and further research on this group of motile aeromonads.

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Selected References

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  1. Altwegg M., Steigerwalt A. G., Altwegg-Bissig R., Lüthy-Hottenstein J., Brenner D. J. Biochemical identification of Aeromonas genospecies isolated from humans. J Clin Microbiol. 1990 Feb;28(2):258–264. doi: 10.1128/jcm.28.2.258-264.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brenner D. J., Fanning G. R., Rake A. V., Johnson K. E. Batch procedure for thermal elution of DNA from hydroxyapatite. Anal Biochem. 1969 Apr 4;28(1):447–459. doi: 10.1016/0003-2697(69)90199-7. [DOI] [PubMed] [Google Scholar]
  3. Brenner D. J., McWhorter A. C., Knutson J. K., Steigerwalt A. G. Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. J Clin Microbiol. 1982 Jun;15(6):1133–1140. doi: 10.1128/jcm.15.6.1133-1140.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carnahan A. M., Marii M. A., Fanning G. R., Pass M. A., Joseph S. W. Characterization of Aeromonas schubertii strains recently isolated from traumatic wound infections. J Clin Microbiol. 1989 Aug;27(8):1826–1830. doi: 10.1128/jcm.27.8.1826-1830.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Farmer J. J., 3rd, Fanning G. R., Huntley-Carter G. P., Holmes B., Hickman F. W., Richard C., Brenner D. J. Kluyvera, a new (redefined) genus in the family Enterobacteriaceae: identification of Kluyvera ascorbata sp. nov. and Kluyvera cryocrescens sp. nov. in clinical specimens. J Clin Microbiol. 1981 May;13(5):919–933. doi: 10.1128/jcm.13.5.919-933.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hazen T. C., Fliermans C. B., Hirsch R. P., Esch G. W. Prevalence and distribution of Aeromonas hydrophila in the United States. Appl Environ Microbiol. 1978 Nov;36(5):731–738. doi: 10.1128/aem.36.5.731-738.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hickman-Brenner F. W., MacDonald K. L., Steigerwalt A. G., Fanning G. R., Brenner D. J., Farmer J. J., 3rd Aeromonas veronii, a new ornithine decarboxylase-positive species that may cause diarrhea. J Clin Microbiol. 1987 May;25(5):900–906. doi: 10.1128/jcm.25.5.900-906.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Janda J. M. Biochemical and exoenzymatic properties of Aeromonas species. Diagn Microbiol Infect Dis. 1985 May;3(3):223–232. doi: 10.1016/0732-8893(85)90034-3. [DOI] [PubMed] [Google Scholar]
  9. Janda J. M., Brenden R. Importance of Aeromonas sobria in Aeromonas bacteremia. J Infect Dis. 1987 Mar;155(3):589–591. doi: 10.1093/infdis/155.3.589. [DOI] [PubMed] [Google Scholar]
  10. Janda J. M., Duffey P. S. Mesophilic aeromonads in human disease: current taxonomy, laboratory identification, and infectious disease spectrum. Rev Infect Dis. 1988 Sep-Oct;10(5):980–997. doi: 10.1093/clinids/10.5.980. [DOI] [PubMed] [Google Scholar]
  11. Janda J. M., Oshiro L. S., Abbott S. L., Duffey P. S. Virulence markers of mesophilic aeromonads: association of the autoagglutination phenomenon with mouse pathogenicity and the presence of a peripheral cell-associated layer. Infect Immun. 1987 Dec;55(12):3070–3077. doi: 10.1128/iai.55.12.3070-3077.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Janda J. M., Reitano M., Bottone E. J. Biotyping of Aeromonas isolates as a correlate to delineating a species-associated disease spectrum. J Clin Microbiol. 1984 Jan;19(1):44–47. doi: 10.1128/jcm.19.1.44-47.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Joseph S. W., Carnahan A. M., Brayton P. R., Fanning G. R., Almazan R., Drabick C., Trudo E. W., Jr, Colwell R. R. Aeromonas jandaei and Aeromonas veronii dual infection of a human wound following aquatic exposure. J Clin Microbiol. 1991 Mar;29(3):565–569. doi: 10.1128/jcm.29.3.565-569.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Joseph S. W., Daily O. P., Hunt W. S., Seidler R. J., Allen D. A., Colwell R. R. Aeromonas primary wound infection of a diver in polluted waters. J Clin Microbiol. 1979 Jul;10(1):46–49. doi: 10.1128/jcm.10.1.46-49.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kaper J. B., Lockman H., Colwell R. R., Joseph S. W. Aeromonas hydrophila: ecology and toxigenicity of isolates from an estuary. J Appl Bacteriol. 1981 Apr;50(2):359–377. doi: 10.1111/j.1365-2672.1981.tb00900.x. [DOI] [PubMed] [Google Scholar]
  16. Namdari H., Bottone E. J. Microbiologic and clinical evidence supporting the role of Aeromonas caviae as a pediatric enteric pathogen. J Clin Microbiol. 1990 May;28(5):837–840. doi: 10.1128/jcm.28.5.837-840.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Popoff M., Véron M. A taxonomic study of the Aeromonas hydrophila-Aeromonas punctata group. J Gen Microbiol. 1976 May;94(1):11–22. doi: 10.1099/00221287-94-1-11. [DOI] [PubMed] [Google Scholar]
  18. Richardson C. J., Robinson J. O., Wagener L. B., Burke V. In-vitro susceptibility of Aeromonas app. to antimicrobial agents. J Antimicrob Chemother. 1982 Apr;9(4):267–274. doi: 10.1093/jac/9.4.267. [DOI] [PubMed] [Google Scholar]
  19. Turnbull P. C., Lee J. V., Miliotis M. D., Van de Walle S., Koornhof H. J., Jeffery L., Bryant T. N. Enterotoxin production in relation to taxonomic grouping and source of isolation of Aeromonas species. J Clin Microbiol. 1984 Feb;19(2):175–180. doi: 10.1128/jcm.19.2.175-180.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. VERON M., GASSER F. SUR LA D'ETECTION DE L'HYDROG'ENE SULFUR'E PRODUIT PAR CERTAINES ENT'EROBACT'ERIAC'EES DANS LES MILIEUX DITS DE DIAGNOSTIC RAPIDE. Ann Inst Pasteur (Paris) 1963 Sep;105:524–534. [PubMed] [Google Scholar]

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