Abstract
We have prepared a murine monoclonal antibody (MAb) capable of distinguishing between wild-type Venezuelan equine encephalomyelitis (VEE) virus and the TC-83 vaccine derivative. This MAb, 1A2B-10, was derived from immunization with a synthetic peptide corresponding to the first 19 amino acids of the E2 glycoprotein of Trinidad donkey VEE virus. The MAb reacts with prototype viruses from all naturally occurring VEE subtypes except subtype 6 in an enzyme-linked immunosorbent assay. It does not react with TC-83 virus or members of the western and eastern equine encephalitis virus complex or with Semliki Forest virus. This antibody will also differentiate between TC-83 and Trinidad donkey VEE virus in indirect immunofluorescence assays with virus-infected Vero cells.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hunt A. R., Johnson A. J., Roehrig J. T. Synthetic peptides of Venezuelan equine encephalomyelitis virus E2 glycoprotein. I. Immunogenic analysis and identification of a protective peptide. Virology. 1990 Dec;179(2):701–711. doi: 10.1016/0042-6822(90)90137-g. [DOI] [PubMed] [Google Scholar]
- Johnson K. M., Martin D. H. Venezuelan equine encephalitis. Adv Vet Sci Comp Med. 1974;18(0):79–116. [PubMed] [Google Scholar]
- Pedersen C. E., Jr, Robinson D. M., Cole F. E., Jr Isolation of the vaccine strain of Venezuelan equine encephalomyelitis virus from mosquitoes in Louisiana. Am J Epidemiol. 1972 May;95(5):490–496. doi: 10.1093/oxfordjournals.aje.a121416. [DOI] [PubMed] [Google Scholar]
- Rico-Hesse R., Roehrig J. T., Dickerman R. W. Monoclonal antibodies define antigenic variation in the ID variety of Venezuelan equine encephalitis virus. Am J Trop Med Hyg. 1988 Jan;38(1):187–194. doi: 10.4269/ajtmh.1988.38.187. [DOI] [PubMed] [Google Scholar]
- Roehrig J. T., Corser J. A., Schlesinger M. J. Isolation and characterization of hybrid cell lines producing monoclonal antibodies directed against the structural proteins of Sindbis virus. Virology. 1980 Feb;101(1):41–49. doi: 10.1016/0042-6822(80)90481-x. [DOI] [PubMed] [Google Scholar]
- Roehrig J. T., Day J. W., Kinney R. M. Antigenic analysis of the surface glycoproteins of a Venezuelan equine encephalomyelitis virus (TC-83) using monoclonal antibodies. Virology. 1982 Apr 30;118(2):269–278. doi: 10.1016/0042-6822(82)90346-4. [DOI] [PubMed] [Google Scholar]
- Roehrig J. T., Hunt A. R., Chang G. J., Sheik B., Bolin R. A., Tsai T. F., Trent D. W. Identification of monoclonal antibodies capable of differentiating antigenic varieties of eastern equine encephalitis viruses. Am J Trop Med Hyg. 1990 Apr;42(4):394–398. doi: 10.4269/ajtmh.1990.42.394. [DOI] [PubMed] [Google Scholar]
- Roehrig J. T., Mathews J. H. The neutralization site on the E2 glycoprotein of Venezuelan equine encephalomyelitis (TC-83) virus is composed of multiple conformationally stable epitopes. Virology. 1985 Apr 30;142(2):347–356. doi: 10.1016/0042-6822(85)90343-5. [DOI] [PubMed] [Google Scholar]
- Roehrig J. T., Mathews J. H., Trent D. W. Identification of epitopes on the E glycoprotein of Saint Louis encephalitis virus using monoclonal antibodies. Virology. 1983 Jul 15;128(1):118–126. doi: 10.1016/0042-6822(83)90323-9. [DOI] [PubMed] [Google Scholar]
- Wiebe M. E., Scherer W. F., Peick W. J. Marker characteristics of Venezuelan encephalitis virus strains isolated before and after epidemics and equine epizootics in Middle America. Am J Epidemiol. 1983 Feb;117(2):201–212. doi: 10.1093/oxfordjournals.aje.a113531. [DOI] [PubMed] [Google Scholar]
- Young N. A., Johnson K. M. Antigenic variants of Venezuelan equine encephalitis virus: their geographic distribution and epidemiologic significance. Am J Epidemiol. 1969 Mar;89(3):286–307. doi: 10.1093/oxfordjournals.aje.a120942. [DOI] [PubMed] [Google Scholar]