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. 1991 Mar;29(3):650–652. doi: 10.1128/jcm.29.3.650-652.1991

Comparison of standard culture methods, a shell vial assay, and a DNA probe for the detection of herpes simplex virus.

L A Seal 1, P S Toyama 1, K M Fleet 1, K S Lerud 1, S R Heth 1, A J Moorman 1, J C Woods 1, R B Hill 1
PMCID: PMC269839  PMID: 1645373

Abstract

A nonradioactive, biotinylated herpes simplex virus (HSV) DNA probe, a shell vial (rabbit kidney cell) culture assay enhanced by a direct fluorescent (HSV monoclonal)-antibody stain at 16 to 20 h postinoculation, and conventional tube cultures with confirmation via HSV-specific (polyclonal antibody) immunoperoxidase assay were compared for 199 specimens. The predictive values of the positive results were 54.5% for the probe, 95.9% for the shell vial assay, and 100% for the conventional culture methods, while the predictive values of the negative tests were 68.1, 84.0, and 98.4%, respectively. We conclude that the DNA probe (sensitivity, 24.5%; specificity, 88.3%) and the shell vial assay (sensitivity, 66.2%; specificity, 98.4%) cannot be substituted for conventional tube culture techniques (sensitivity, 97.1%; specificity, 100%) in the routine identification of HSV in our laboratory.

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Selected References

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