. 2009 May 2;59(2):81–91. doi: 10.1007/s10616-009-9196-x

Table 1.

Summary of growth and production characteristics of SEAP-producing CHO cells in response to temperature reduction

Culture conditions	Specific growth rate (h⁻¹)	Max. viable cell conc. (10⁶ cells/mL)	q_SEAP (10⁻⁶U/cells/day)		Max. SEAP activity (U/mL)		Max. volumetric productivity (U/mL/day)		Max. IVCD (10⁶cells/mL × day)		Culture Time^e (h)
			Single^b		Single	Bi^b	Single	Bi	Single	Bi	Single	Bi
			Before^c	After^d	Single	Bi^b	Single	Bi	Single	Bi	Single	Bi
Suspension	0.032	1.65	0.045		0.20	0.68	0.034	0.077	6.2	10	135	212
Suspension	0.032	1.65	0.013	0.105	0.20	0.68	0.034	0.077	6.2	10	135	212
Microcarrier (Cytodex 3)	0.029 −9%^a	1.25 −24%	0.027		0.12	0.27 −60%	0.017	0.027 −65%	5.4	8.0 −21%	164	241 +14%
Microcarrier (Cytodex 3)	0.029 −9%^a	1.25 −24%	0.017 +31%	0.042 −60%	0.12	0.27 −60%	0.017	0.027 −65%	5.4	8.0 −21%	164	241 +14%
Microcarrier (Cytopore 1)^f	0.025 −22%	1.14 −31%	0.017		0.11	0.15 −78%	0.013	0.018 −77%	6.7	7.2 −29%	184	203 n.a.
Microcarrier (Cytopore 1)^f	0.025 −22%	1.14 −31%	0.010 −23%	0.030 −71%	0.11	0.15 −78%	0.013	0.018 −77%	6.7	7.2 −29%	184	203 n.a.

^aPercent change compared to suspension culture

^bSingle: single-temperature cultures; Bi: biphasic cultures

^cSpecific productivity before low temperature shift

^dSpecific productivity after low temperature shift

^eCulture time is the time when culture viability decreases to ~60%; for Cytopore 1 culture, the time is termination time

^fData based on the total cell density