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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1991 Apr;29(4):718–722. doi: 10.1128/jcm.29.4.718-722.1991

Evaluation of the Baxter-MicroScan 4-hour enzyme-based yeast identification system.

G A Land 1, I F Salkin 1, M el-Zaatari 1, M R McGinnis 1, G Hashem 1
PMCID: PMC269859  PMID: 1653786

Abstract

A new 4-h Yeast Identification Panel (YIP; Baxter-MicroScan, W. Sacramento, Calif.) was compared with the API 20C Yeast Identification System (Analytab Products, Inc., Plainview, N.Y.) in the identification of recent clinical yeast isolates. The YIP had a 94% correlation (288 of 306) in identifying 22 species within the genera Candida, Hansenula, Pichia, Rhodotorula, Saccharomyces, and Torulopsis. Correlation dropped to 65% for those species within the genera of slower growing yeasts, i.e., Blastoschizomyces spp., Crpytococcus spp., Geotrichum spp., Hyphopichia spp., Phaeococcomyces spp., Prototheca spp., and Trichosporon spp. Overall correlation with the API 20C was 92% (365 of 401) for those taxa included in the data base and 85% (373 of 437) for all yeasts encountered in the study. There were 36 (8.2%) discrepant identifications, which were due in part to the limited data base. Expansion of the data base plus the easy inoculation, reading, and rapid results of the YIP should make it an excellent method for yeast identification.

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Selected References

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  1. BLOOMFIELD N., GORDON M. A., ELMENDORF D. F., Jr DETECTION OF CRYPTOCOCCUS NEOFORMANS ANTIGEN IN BODY FLUIDS BY LATEX PARTICLE AGGLUTINATION. Proc Soc Exp Biol Med. 1963 Oct;114:64–67. doi: 10.3181/00379727-114-28586. [DOI] [PubMed] [Google Scholar]
  2. Baker J. G., Salkin I. F., Pincus D. H., Bodensteiner D. C., D'Amato R. F. Pathogenicity of Candida paratropicalis. Arch Pathol Lab Med. 1983 Nov;107(11):577–579. [PubMed] [Google Scholar]
  3. Bobey D. G., Ederer G. M. Rapid detection of yeast enzymes by using 4-methylumbelliferyl substrates. J Clin Microbiol. 1981 Feb;13(2):393–394. doi: 10.1128/jcm.13.2.393-394.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bowman P. I., Ahearn D. G. Evaluation of the Uni-Yeast-Tek kit for the identification of medically important yeasts. J Clin Microbiol. 1975 Oct;2(4):354–358. doi: 10.1128/jcm.2.4.354-358.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Casal M., Linares M. J. Contribution to the study of the enzymatic profiles of yeast organisms with medical interest. Mycopathologia. 1983 Mar 22;81(3):155–159. doi: 10.1007/BF00436820. [DOI] [PubMed] [Google Scholar]
  6. Christenson J. C., Guruswamy A., Mukwaya G., Rettig P. J. Candida lusitaniae: an emerging human pathogen. Pediatr Infect Dis J. 1987 Aug;6(8):755–757. [PubMed] [Google Scholar]
  7. Cooper B. H., Prowant S., Alexander B., Brunson D. H. Collaborative evaluation of the Abbott yeast identification system. J Clin Microbiol. 1984 Jun;19(6):853–856. doi: 10.1128/jcm.19.6.853-856.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hadfield T. L., Smith M. B., Winn R. E., Rinaldi M. G., Guerra C. Mycoses caused by Candida lusitaniae. Rev Infect Dis. 1987 Sep-Oct;9(5):1006–1012. doi: 10.1093/clinids/9.5.1006. [DOI] [PubMed] [Google Scholar]
  9. Hoy J., Hsu K. C., Rolston K., Hopfer R. L., Luna M., Bodey G. P. Trichosporon beigelii infection: a review. Rev Infect Dis. 1986 Nov-Dec;8(6):959–967. [PubMed] [Google Scholar]
  10. Land G. A., Harrison B. A., Hulme K. L., Cooper B. H., Byrd J. C. Evaluation of the new API 20C strip for yeast identification against a conventional method. J Clin Microbiol. 1979 Sep;10(3):357–364. doi: 10.1128/jcm.10.3.357-364.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Land G. A., Salkin I. F. Uncommon yeastlike zoopathogens and commercial systems for their identification. Mycopathologia. 1987 Sep;99(3):155–171. doi: 10.1007/BF00437438. [DOI] [PubMed] [Google Scholar]
  12. Land G. A., Vinton E. C., Adcock G. B., Hopkins J. M. Improved auxanographic method for yeast assimilations: a comparison with other approaches. J Clin Microbiol. 1975 Sep;2(3):206–217. doi: 10.1128/jcm.2.3.206-217.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pappagianis D., Collins M. S., Hector R., Remington J. Development of resistance to amphotericin B in Candida lusitaniae infecting a human. Antimicrob Agents Chemother. 1979 Aug;16(2):123–126. doi: 10.1128/aac.16.2.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pfaller M. A., Preston T., Bale M., Koontz F. P., Body B. A. Comparison of the Quantum II, API Yeast Ident, and AutoMicrobic systems for identification of clinical yeast isolates. J Clin Microbiol. 1988 Oct;26(10):2054–2058. doi: 10.1128/jcm.26.10.2054-2058.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Roberts G. D., Wang H. S., Hollick G. E. Evaluation of the API 20 c microtube system for the identification of clinically important yeasts. J Clin Microbiol. 1976 Mar;3(3):302–305. doi: 10.1128/jcm.3.3.302-305.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Salkin I. F., Land G. A., Hurd N. J., Goldson P. R., McGinnis M. R. Evaluation of YeastIdent and Uni-Yeast-Tek yeast identification systems. J Clin Microbiol. 1987 Apr;25(4):624–627. doi: 10.1128/jcm.25.4.624-627.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schlitzer R. L., Ahearn D. G. Characterization of atypical Candida tropicalis and other uncommon clinical yeast isolates. J Clin Microbiol. 1982 Mar;15(3):511–516. doi: 10.1128/jcm.15.3.511-516.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. St-Germain G., Laverdière M. Torulopsis candida, a new opportunistic pathogen. J Clin Microbiol. 1986 Nov;24(5):884–885. doi: 10.1128/jcm.24.5.884-885.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Walsh T. J., Pizzo P. A. Nosocomial fungal infections: a classification for hospital-acquired fungal infections and mycoses arising from endogenous flora or reactivation. Annu Rev Microbiol. 1988;42:517–545. doi: 10.1146/annurev.mi.42.100188.002505. [DOI] [PubMed] [Google Scholar]
  20. Wingard J. R., Merz W. G., Saral R. Candida tropicalis: a major pathogen in immunocompromised patients. Ann Intern Med. 1979 Oct;91(4):539–543. doi: 10.7326/0003-4819-91-4-539. [DOI] [PubMed] [Google Scholar]
  21. Zimmer B. L., Roberts G. D. Rapid selective urease test for presumptive identification of Cryptococcus neoformans. J Clin Microbiol. 1979 Sep;10(3):380–381. doi: 10.1128/jcm.10.3.380-381.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. el-Zaatari M., Pasarell L., McGinnis M. R., Buckner J., Land G. A., Salkin I. F. Evaluation of the updated Vitek yeast identification data base. J Clin Microbiol. 1990 Sep;28(9):1938–1941. doi: 10.1128/jcm.28.9.1938-1941.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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