Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1991 Apr;29(4):778–781. doi: 10.1128/jcm.29.4.778-781.1991

Seroreactive recombinant herpes simplex virus type 2-specific glycoprotein G.

D L Parkes 1, C M Smith 1, J M Rose 1, J Brandis 1, S R Coates 1
PMCID: PMC269869  PMID: 1653787

Abstract

The herpes simplex virus type 2 (HSV-2) genome codes for an envelope protein, glycoprotein G (gG), which contains predominantly type 2-specific epitopes. A portion of this gG gene has been expressed as a fusion protein in Escherichia coli. Expression was regulated by a lambda phage pL promoter. The 60,000-molecular-weight recombinant protein was purified by ion-exchange chromatography. Amino acid sequence analysis confirmed the N terminus of the purified protein. Mice immunized with recombinant gG developed antibodies reactive with native HSV-2 protein, but not with HSV-1 protein, in an indirect immunofluorescence assay. The serological activity of this purified recombinant gG protein was evaluated by immunoblot assay. This protein was reactive with an HSV-2 gG monoclonal antibody. It was also reactive with HSV-2 rabbit antiserum but not with HSV-1 rabbit antiserum. Of 15 patient serum samples known to have antibody to HSV-2, 14 were reactive with this recombinant type 2-specific gG protein, and none of 15 HSV antibody-negative patient serum samples showed reactivity. In agreement with the expected prevalence of HSV-2 infection, 27.6% of 134 serum samples from random normal individuals had antibodies reactive with recombinant gG. This recombinant gG protein may be of value in detecting HSV-2-specific antibody responses in patients infected with HSV-2.

Full text

PDF
778

Images in this article

779Image
on p.779
780Image
on p.780

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashley R. L., Militoni J., Lee F., Nahmias A., Corey L. Comparison of Western blot (immunoblot) and glycoprotein G-specific immunodot enzyme assay for detecting antibodies to herpes simplex virus types 1 and 2 in human sera. J Clin Microbiol. 1988 Apr;26(4):662–667. doi: 10.1128/jcm.26.4.662-667.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Balachandran N., Hutt-Fletcher L. M. Synthesis and processing of glycoprotein gG of herpes simplex virus type 2. J Virol. 1985 Jun;54(3):825–832. doi: 10.1128/jvi.54.3.825-832.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Corey L., Adams H. G., Brown Z. A., Holmes K. K. Genital herpes simplex virus infections: clinical manifestations, course, and complications. Ann Intern Med. 1983 Jun;98(6):958–972. doi: 10.7326/0003-4819-98-6-958. [DOI] [PubMed] [Google Scholar]
  4. Forghani B., Schmidt N. J., Lennette E. H. Solid phase radioimmunoassay for typing herpes simplex viral antibodies in human sera. J Clin Microbiol. 1975 Nov;2(5):410–418. doi: 10.1128/jcm.2.5.410-418.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Johnson R. E., Nahmias A. J., Magder L. S., Lee F. K., Brooks C. A., Snowden C. B. A seroepidemiologic survey of the prevalence of herpes simplex virus type 2 infection in the United States. N Engl J Med. 1989 Jul 6;321(1):7–12. doi: 10.1056/NEJM198907063210102. [DOI] [PubMed] [Google Scholar]
  6. Langenberg A., Benedetti J., Jenkins J., Ashley R., Winter C., Corey L. Development of clinically recognizable genital lesions among women previously identified as having "asymptomatic" herpes simplex virus type 2 infection. Ann Intern Med. 1989 Jun 1;110(11):882–887. doi: 10.7326/0003-4819-110-11-882. [DOI] [PubMed] [Google Scholar]
  7. Lee F. K., Coleman R. M., Pereira L., Bailey P. D., Tatsuno M., Nahmias A. J. Detection of herpes simplex virus type 2-specific antibody with glycoprotein G. J Clin Microbiol. 1985 Oct;22(4):641–644. doi: 10.1128/jcm.22.4.641-644.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lehtinen M., Koivisto V., Lehtinen T., Paavonen J., Leinikki P. Immunoblotting and enzyme-linked immunosorbent assay analysis of serological responses in patients infected with herpes simplex virus types 1 and 2. Intervirology. 1985;24(1):18–25. doi: 10.1159/000149614. [DOI] [PubMed] [Google Scholar]
  9. Marsden H. S., Buckmaster A., Palfreyman J. W., Hope R. G., Minson A. C. Characterization of the 92,000-dalton glycoprotein induced by herpes simplex virus type 2. J Virol. 1984 May;50(2):547–554. doi: 10.1128/jvi.50.2.547-554.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McGeoch D. J., Moss H. W., McNab D., Frame M. C. DNA sequence and genetic content of the HindIII l region in the short unique component of the herpes simplex virus type 2 genome: identification of the gene encoding glycoprotein G, and evolutionary comparisons. J Gen Virol. 1987 Jan;68(Pt 1):19–38. doi: 10.1099/0022-1317-68-1-19. [DOI] [PubMed] [Google Scholar]
  11. Moore D. E., Ashley R. L., Zarutskie P. W., Coombs R. W., Soules M. R., Corey L. Transmission of genital herpes by donor insemination. JAMA. 1989 Jun 16;261(23):3441–3443. [PubMed] [Google Scholar]
  12. Plummer G. A review of the identification and titration of antibodies to herpes simplex viruses type 1 and type 2 in human sera. Cancer Res. 1973 Jun;33(6):1469–1476. [PubMed] [Google Scholar]
  13. Roizman B., Norrild B., Chan C., Pereira L. Identification and preliminary mapping with monoclonal antibodies of a herpes simplex virus 2 glycoprotein lacking a known type 1 counterpart. Virology. 1984 Feb;133(1):242–247. doi: 10.1016/0042-6822(84)90447-1. [DOI] [PubMed] [Google Scholar]
  14. Seth A., Lapis P., Vande Woude G. F., Papas T. High-level expression vectors to synthesize unfused proteins in Escherichia coli. Gene. 1986;42(1):49–57. doi: 10.1016/0378-1119(86)90149-6. [DOI] [PubMed] [Google Scholar]
  15. Sullender W. M., Yasukawa L. L., Schwartz M., Pereira L., Hensleigh P. A., Prober C. G., Arvin A. M. Type-specific antibodies to herpes simplex virus type 2 (HSV-2) glycoprotein G in pregnant women, infants exposed to maternal HSV-2 infection at delivery, and infants with neonatal herpes. J Infect Dis. 1988 Jan;157(1):164–171. doi: 10.1093/infdis/157.1.164. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES