Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1991 May;29(5):879–882. doi: 10.1128/jcm.29.5.879-882.1991

Controlled evaluation of BACTEC Plus 26 and Roche Septi-Chek aerobic blood culture bottles.

M P Weinstein 1, S Mirrett 1, M L Wilson 1, L J Harrell 1, C W Stratton 1, L B Reller 1
PMCID: PMC269900  PMID: 2056053

Abstract

Blood culture bottles that accept up to 10 ml of blood have recently been introduced for the BACTEC nonradiometric blood culture system. The new formulation, designated BACTEC Plus, contains 25 ml of tryptic soy broth, 0.05% sodium polyanetholesulfonate, and mixed resins. In a collaborative study conducted at three university hospitals, we evaluated the BACTEC Plus 26 (BP26) aerobic bottle and the Roche Septi-Chek aerobic bottle with its agar slide paddle in 5,293 paired blood cultures. Significantly more microorganisms (P less than 0.001), especially Staphylococcus aureus (P less than 0.001), Staphylococcus epidermidis (P less than 0.01), enterococci (P less than 0.005), and members of the family Enterobacteriaceae (P less than 0.005), were detected by the BP26 bottle. When both bottles detected growth, BP26 did so earlier (P less than 0.001). In particular, S. epidermidis (P less than 0.001), streptococci (P less than 0.005), enterococci (P less than 0.05), and members of the family Enterobacteriaceae (P less than 0.001) were detected earlier by the BP26 bottle. We conclude that the BP26 bottle provides a yield and speed of detection of microorganisms superior to those of the Roche Septi-Chek aerobic blood culture bottle.

Full text

PDF
879

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appleman M. D., Swinney R. S., Heseltine P. N. Evaluation of the Antibiotic Removal Device. J Clin Microbiol. 1982 Feb;15(2):278–281. doi: 10.1128/jcm.15.2.278-281.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aronson M. D., Bor D. H. Blood cultures. Ann Intern Med. 1987 Feb;106(2):246–253. doi: 10.7326/0003-4819-106-2-246. [DOI] [PubMed] [Google Scholar]
  3. Courcol R. J., Durocher A. V., Roussel-Delvallez M., Fruchart A., Martin G. R. Routine evaluation of BACTEC NR-16A and NR-17A media. J Clin Microbiol. 1988 Sep;26(9):1619–1622. doi: 10.1128/jcm.26.9.1619-1622.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Doern G. V., Gantz N. M. Detection of bacteremia in patients receiving antimicrobial therapy: an evaluation of the antimicrobial removal device and 16B medium. J Clin Microbiol. 1983 Jul;18(1):43–48. doi: 10.1128/jcm.18.1.43-48.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Henry N. K., McLimans C. A., Wright A. J., Thompson R. L., Wilson W. R., Washington J. A., 2nd Microbiological and clinical evaluation of the isolator lysis-centrifugation blood culture tube. J Clin Microbiol. 1983 May;17(5):864–869. doi: 10.1128/jcm.17.5.864-869.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hopfer R. L., Moore D. G., Fainstein V., Watkins S., Wenglar M. Routine use of BACTEC 16B bottles to remove antibacterial and antitumor agents from blood cultures of cancer patients. J Clin Microbiol. 1983 Oct;18(4):759–764. doi: 10.1128/jcm.18.4.759-764.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ilstrup D. M., Washington J. A., 2nd The importance of volume of blood cultured in the detection of bacteremia and fungemia. Diagn Microbiol Infect Dis. 1983 Jun;1(2):107–110. doi: 10.1016/0732-8893(83)90039-1. [DOI] [PubMed] [Google Scholar]
  8. Lorian V. Some effects of subinhibitory concentrations of antibiotics on bacteria. Bull N Y Acad Med. 1975 Oct;51(9):1046–1055. [PMC free article] [PubMed] [Google Scholar]
  9. McGuire N. M., Kauffman C. A., Hertz C. S., Kovach J. M. Evaluation of the BACTEC antimicrobial removal system for detection of bacteremia. J Clin Microbiol. 1983 Sep;18(3):449–451. doi: 10.1128/jcm.18.3.449-451.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pfaller M. A., Sibley T. K., Westfall L. M., Hoppe-Bauer J. E., Keating M. A., Murray P. R. Clinical laboratory comparison of a slide blood culture system with a conventional broth system. J Clin Microbiol. 1982 Sep;16(3):525–530. doi: 10.1128/jcm.16.3.525-530.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Plorde J. J., Carlson L. G., Dau M. E. Lack of clinical relevance in routine final subcultures of radiometrically negative BACTEC blood culture vials. Am J Clin Pathol. 1982 Nov;78(5):753–755. doi: 10.1093/ajcp/78.5.753. [DOI] [PubMed] [Google Scholar]
  12. Plorde J. J., Tenover F. C., Carlson L. G. Specimen volume versus yield in the BACTEC blood culture system. J Clin Microbiol. 1985 Aug;22(2):292–295. doi: 10.1128/jcm.22.2.292-295.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Reimer L. G., McDaniel J. D., Mirrett S., Reller L. B., Wang W. L. Controlled evaluation of supplemented peptone and Bactec blood culture broths for the detection of bacteremia and fungemia. J Clin Microbiol. 1985 Apr;21(4):531–534. doi: 10.1128/jcm.21.4.531-534.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Strand C. L., Hickman A., Bryant J. K., Sutton K. Evaluation of the BACTEC resin blood-culture medium. Am J Clin Pathol. 1983 Nov;80(5):706–710. doi: 10.1093/ajcp/80.5.706. [DOI] [PubMed] [Google Scholar]
  15. Tenney J. H., Reller L. B., Mirrett S., Wang W. L., Weinstein M. P. Controlled evaluation of the volume of blood cultured in detection of bacteremia and fungemia. J Clin Microbiol. 1982 Apr;15(4):558–561. doi: 10.1128/jcm.15.4.558-561.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Washington J. A., 2nd, Ilstrup D. M. Blood cultures: issues and controversies. Rev Infect Dis. 1986 Sep-Oct;8(5):792–802. doi: 10.1093/clinids/8.5.792. [DOI] [PubMed] [Google Scholar]
  17. Weinstein M. P., Mirrett S., Reller L. B. Comparative evaluation of Oxoid Signal and BACTEC radiometric blood culture systems for the detection of bacteremia and fungemia. J Clin Microbiol. 1988 May;26(5):962–964. doi: 10.1128/jcm.26.5.962-964.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Weinstein M. P., Reller L. B., Mirrett S., Stratton C. W., Reimer L. G., Wang W. L. Controlled evaluation of the agar-slide and radiometric blood culture systems for the detection of bacteremia and fungemia. J Clin Microbiol. 1986 Feb;23(2):221–225. doi: 10.1128/jcm.23.2.221-225.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weinstein M. P., Reller L. B., Murphy J. R., Lichtenstein K. A. The clinical significance of positive blood cultures: a comprehensive analysis of 500 episodes of bacteremia and fungemia in adults. I. Laboratory and epidemiologic observations. Rev Infect Dis. 1983 Jan-Feb;5(1):35–53. doi: 10.1093/clinids/5.1.35. [DOI] [PubMed] [Google Scholar]
  20. Wright A. J., Thompson R. L., McLimans C. A., Wilson W. R., Washington J. A., 2nd The antimicrobial removal device. A microbiological and clinical evaluation. Am J Clin Pathol. 1982 Aug;78(2):173–177. doi: 10.1093/ajcp/78.2.173. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES