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. 1991 May;29(5):889–893. doi: 10.1128/jcm.29.5.889-893.1991

Analysis of serotypes and electropherotypes of equine rotaviruses isolated in the United States.

M E Hardy 1, G N Woode 1, Z C Xu 1, J D Williams 1, M E Conner 1, R M Dwyer 1, D G Powell 1
PMCID: PMC269902  PMID: 1647407

Abstract

Equine group A rotaviruses isolated over a 10-year period in New York State, New Jersey, Kentucky, and Texas were compared serotypically and electropherotypically. All isolates were determined to be serotype 3 by reaction with hyperimmune antiserum to the serotype 3 H-2 strain of equine rotavirus. All displayed RNA electrophoretic migration patterns related to that of the H-2 strain but distinct from that of serotype 5 strain H-1. A serologic survey of 184 mares in Kentucky, which was done to determine the incidence of H-1 and H-2 infections, showed geometric mean serum neutralizing titers to the H-2 strain of equine rotavirus to be significantly higher than those to the H-1 strain. These data suggest that the serotype 3 H-2 strain is the dominant equine rotavirus in Kentucky and perhaps elsewhere in the United States. We were unable to produce confirmational evidence that the H-1 strain occurs as a natural infection in the United States.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beards G. M. Polymorphism of genomic RNAs within rotavirus serotypes and subgroups. Arch Virol. 1982;74(1):65–70. doi: 10.1007/BF01320783. [DOI] [PubMed] [Google Scholar]
  2. Conner M. E., Darlington R. W. Rotavirus infection in foals. Am J Vet Res. 1980 Oct;41(10):1699–1703. [PubMed] [Google Scholar]
  3. Durham P. J., Stevenson B. J., Farquharson B. C. Rotavirus and coronavirus associated diarrhoea in domestic animals. N Z Vet J. 1979 Mar;27(3):30–32. doi: 10.1080/00480169.1979.34595. [DOI] [PubMed] [Google Scholar]
  4. Estes M. K., Graham D. Y., Dimitrov D. H. The molecular epidemiology of rotavirus gastroenteritis. Prog Med Virol. 1984;29:1–22. [PubMed] [Google Scholar]
  5. Gaul S. K., Simpson T. F., Woode G. N., Fulton R. W. Antigenic relationships among some animal rotaviruses: virus neutralization in vitro and cross-protection in piglets. J Clin Microbiol. 1982 Sep;16(3):495–503. doi: 10.1128/jcm.16.3.495-503.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gillespie J., Kalica A., Conner M., Schiff E., Barr M., Holmes D., Frey M. The isolation, propagation and characterization of tissue-cultured equine rotaviruses. Vet Microbiol. 1984 Feb;9(1):1–14. doi: 10.1016/0378-1135(84)90074-9. [DOI] [PubMed] [Google Scholar]
  7. Greenberg H. B., Valdesuso J., van Wyke K., Midthun K., Walsh M., McAuliffe V., Wyatt R. G., Kalica A. R., Flores J., Hoshino Y. Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus. J Virol. 1983 Aug;47(2):267–275. doi: 10.1128/jvi.47.2.267-275.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hoshino Y., Wyatt R. G., Greenberg H. B., Flores J., Kapikian A. Z. Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization. J Infect Dis. 1984 May;149(5):694–702. doi: 10.1093/infdis/149.5.694. [DOI] [PubMed] [Google Scholar]
  9. Hoshino Y., Wyatt R. G., Greenberg H. B., Kalica A. R., Flores J., Kapikian A. Z. Isolation and characterization of an equine rotavirus. J Clin Microbiol. 1983 Sep;18(3):585–591. doi: 10.1128/jcm.18.3.585-591.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hoshino Y., Wyatt R. G., Greenberg H. B., Kalica A. R., Flores J., Kapikian A. Z. Isolation, propagation, and characterization of a second equine rotavirus serotype. Infect Immun. 1983 Sep;41(3):1031–1037. doi: 10.1128/iai.41.3.1031-1037.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Offit P. A., Clark H. F., Blavat G., Greenberg H. B. Reassortant rotaviruses containing structural proteins vp3 and vp7 from different parents induce antibodies protective against each parental serotype. J Virol. 1986 Nov;60(2):491–496. doi: 10.1128/jvi.60.2.491-496.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shaw R. D., Vo P. T., Offit P. A., Coulson B. S., Greenberg H. B. Antigenic mapping of the surface proteins of rhesus rotavirus. Virology. 1986 Dec;155(2):434–451. doi: 10.1016/0042-6822(86)90205-9. [DOI] [PubMed] [Google Scholar]
  13. Theil K. W., McCloskey C. M., Saif L. J., Redman D. R., Bohl E. H., Hancock D. D., Kohler E. M., Moorhead P. D. Rapid, simple method of preparing rotaviral double-stranded ribonucleic acid for analysis by polyacrylamide gel electrophoresis. J Clin Microbiol. 1981 Sep;14(3):273–280. doi: 10.1128/jcm.14.3.273-280.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tzipori S., Walker M. Isolation of rotavirus from foals with diarrhoea. Aust J Exp Biol Med Sci. 1978 Aug;56(4):453–457. doi: 10.1038/icb.1978.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Woode G. N., Bridger J. C., Jones J. M., Flewett T. H., Davies H. A., Davis H. A., White G. B. Morphological and antigenic relationships between viruses (rotaviruses) from acute gastroenteritis of children, calves, piglets, mice, and foals. Infect Immun. 1976 Sep;14(3):804–810. doi: 10.1128/iai.14.3.804-810.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Woode G. N., Kelso N. E., Simpson T. F., Gaul S. K., Evans L. E., Babiuk L. Antigenic relationships among some bovine rotaviruses: serum neutralization and cross-protection in gnotobiotic calves. J Clin Microbiol. 1983 Aug;18(2):358–364. doi: 10.1128/jcm.18.2.358-364.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Woode G. N., Zheng S. L., Rosen B. I., Knight N., Gourley N. E., Ramig R. F. Protection between different serotypes of bovine rotavirus in gnotobiotic calves: specificity of serum antibody and coproantibody responses. J Clin Microbiol. 1987 Jun;25(6):1052–1058. doi: 10.1128/jcm.25.6.1052-1058.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Zheng S. L., Woode G. N., Melendy D. R., Ramig R. F. Comparative studies of the antigenic polypeptide species VP4, VP6, and VP7 of three strains of bovine rotavirus. J Clin Microbiol. 1989 Sep;27(9):1939–1945. doi: 10.1128/jcm.27.9.1939-1945.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

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