Abstract
The ATB 32 Staph system correctly identified 45 of 54 Staphylococcus hyicus cultures isolated from pigs and cattle. The biochemical profiles of the remaining nine cultures were not listed in the product data base. The 40 porcine and 14 bovine cultures resulted in three and seven different biochemical profiles, respectively. In parallel experiments, almost all S. hyicus cultures showed hemolytic reactions on chocolate agar, had CAMP-like reactivities in the zone of lysis of the staphylococcal beta-lysin, and had bacteriolytic properties on Micrococcus luteus cells. In addition, the S. hyicus cultures were unpigmented, were DNase positive, expressed an S. hyicus-specific teichoic acid, and were usually coagulase positive in porcine plasma. Most of the porcine strains were protein A positive, and two porcine cultures were clumping factor positive.
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- Brun Y., Bes M., Boeufgras J. M., Monget D., Fleurette J., Auckenthaler R., Devriese L. A., Kocur M., Marples R. R., Piemont Y. International collaborative evaluation of the ATB 32 staph gallery for identification of the Staphylococcus species. Zentralbl Bakteriol. 1990 Aug;273(3):319–326. doi: 10.1016/s0934-8840(11)80435-4. [DOI] [PubMed] [Google Scholar]
- Crouch S. F., Pearson T. A., Parham D. M. Comparison of modified Minitek system with Staph-Ident system for species identification of coagulase-negative staphylococci. J Clin Microbiol. 1987 Sep;25(9):1626–1628. doi: 10.1128/jcm.25.9.1626-1628.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Devriese L. A., Derycke J. Staphylococcus hyicus in cattle. Res Vet Sci. 1979 May;26(3):356–358. [PubMed] [Google Scholar]
- Devriese L. A. Identification of clumping-factor-negative staphylococci isolated from cows' udders. Res Vet Sci. 1979 Nov;27(3):313–320. [PubMed] [Google Scholar]
- Devriese L. A., Schleifer K. H., Adegoke G. O. Identification of coagulase-negative staphylococci from farm animals. J Appl Bacteriol. 1985 Jan;58(1):45–55. doi: 10.1111/j.1365-2672.1985.tb01428.x. [DOI] [PubMed] [Google Scholar]
- Devriese L. A., Vlaminck K., Nuytten J., De Keersmaecker P. Staphylococcus hyicus in skin lesions of horses. Equine Vet J. 1983 Jul;15(3):263–265. doi: 10.1111/j.2042-3306.1983.tb01786.x. [DOI] [PubMed] [Google Scholar]
- Frede C., Christ D., Lämmler C. Streptolytic activities of a lytic enzyme from Staphylococcus hyicus. Zentralbl Bakteriol. 1989 May;271(1):54–60. doi: 10.1016/s0934-8840(89)80053-2. [DOI] [PubMed] [Google Scholar]
- Hébert G. A., Hancock G. A. Synergistic hemolysis exhibited by species of staphylococci. J Clin Microbiol. 1985 Sep;22(3):409–415. doi: 10.1128/jcm.22.3.409-415.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kloos W. E., Wolfshohl J. F. Identification of Staphylococcus species with the API STAPH-IDENT system. J Clin Microbiol. 1982 Sep;16(3):509–516. doi: 10.1128/jcm.16.3.509-516.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Langlois B. E., Harmon R. J., Akers K. Identification of Staphylococcus species of bovine origin with the DMS Staph-Trac system. J Clin Microbiol. 1984 Aug;20(2):227–230. doi: 10.1128/jcm.20.2.227-230.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lantz M. S., Switalski L. M., Kornman K. S., Hök M. Bacteroides intermedius binds fibrinogen. J Bacteriol. 1985 Aug;163(2):623–628. doi: 10.1128/jb.163.2.623-628.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lämmler C., Brückler J. Synergistic hemolytic reactions between staphylococci and Micrococcus lylae. Zentralbl Veterinarmed B. 1989 Jun;36(4):297–302. doi: 10.1111/j.1439-0450.1989.tb00605.x. [DOI] [PubMed] [Google Scholar]
- Lämmler C. Characteristic bacteriolytic activities of Staphylococcus hyicus. J Clin Microbiol. 1989 Jul;27(7):1682–1683. doi: 10.1128/jcm.27.7.1682-1683.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lämmler C. Evaluation of the Staph-Zym-system for identification of Staphylococcus hyicus and Staphylococcus intermedius. Zentralbl Veterinarmed B. 1989 May;36(3):180–184. doi: 10.1111/j.1439-0450.1989.tb00589.x. [DOI] [PubMed] [Google Scholar]
- Lämmler C., de Freitas J. C., Chhatwal G. S., Blobel H. Interactions of immunoglobulin G, fibrinogen and fibronectin with Staphylococcus hyicus and Staphylococcus intermedius. Zentralbl Bakteriol Mikrobiol Hyg A. 1985 Oct;260(2):232–237. doi: 10.1016/s0176-6724(85)80119-x. [DOI] [PubMed] [Google Scholar]
- Maddux R. L., Koehne G. Identification of Staphylococcus hyicus with the API staph strip. J Clin Microbiol. 1982 Jun;15(6):984–986. doi: 10.1128/jcm.15.6.984-986.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miekka S. I., Ingham K. C., Menache D. Rapid methods for isolation of human plasma fibronectin. Thromb Res. 1982 Jul 1;27(1):1–14. doi: 10.1016/0049-3848(82)90272-9. [DOI] [PubMed] [Google Scholar]
- Müller H. P., Schaeg W., Blobel H. Protein A-Aktivität von Staphylococcus hyicus im Vergleich zu Protein A von Staphylococcus aureus. Zentralbl Bakteriol Mikrobiol Hyg A. 1981;249(4):443–451. [PubMed] [Google Scholar]
- Phillips W. E., Jr, Kloos W. E. Identification of coagulase-positive Staphylococcus intermedius and Staphylococcus hyicus subsp. hyicus isolates from veterinary clinical specimens. J Clin Microbiol. 1981 Dec;14(6):671–673. doi: 10.1128/jcm.14.6.671-673.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shimizu A., Teranishi H., Kawano J., Kimura S. Phage patterns of Staphylococcus hyicus subsp. hyicus isolated from chickens, cattle and pigs. Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Jun;265(1-2):57–61. doi: 10.1016/s0176-6724(87)80152-9. [DOI] [PubMed] [Google Scholar]
- Takeuchi S., Kobayashi Y., Morozumi T., Niibori S. Isolation and some properties of Staphylococcus hyicus subsp. hyicus from pigs, chickens and cows. Nihon Juigaku Zasshi. 1985 Oct;47(5):841–843. doi: 10.1292/jvms1939.47.841. [DOI] [PubMed] [Google Scholar]
- Watts J. L., Nickerson S. C. A comparison of the STAPH-Ident and STAPH-Trac systems to conventional methods in the identification of staphylococci isolated from bovine udders. Vet Microbiol. 1986 Jul;12(2):179–187. doi: 10.1016/0378-1135(86)90079-9. [DOI] [PubMed] [Google Scholar]
- Watts J. L., Owens W. E., Nickerson S. C. Evaluation of the Minitek gram-positive set for identification of staphylococci isolated from the bovine mammary gland. J Clin Microbiol. 1986 May;23(5):873–875. doi: 10.1128/jcm.23.5.873-875.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watts J. L., Owens W. E., Nickerson S. C. Identification of staphylococci from bovine udders: evaluation of the API 20GP system. Can J Microbiol. 1986 Apr;32(4):359–361. doi: 10.1139/m86-069. [DOI] [PubMed] [Google Scholar]
- Watts J. L., Pankey J. W., Nickerson S. C. Evaluation of the Staph-Ident and STAPHase systems for identification of staphylococci from bovine intramammary infections. J Clin Microbiol. 1984 Sep;20(3):448–452. doi: 10.1128/jcm.20.3.448-452.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]


