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. 1991 Jul;29(7):1303–1307. doi: 10.1128/jcm.29.7.1303-1307.1991

Comparison of ribotyping with conventional methods for the type identification of Enterobacter cloacae.

J Garaizar 1, M E Kaufmann 1, T L Pitt 1
PMCID: PMC270105  PMID: 1715880

Abstract

An Escherichia coli rRNA probe was compared with a combination of O serotyping, phage susceptibility, and biotype pattern for the type identification of strains of Enterobacter cloacae. Forty-five isolates of E. cloacae from 36 patients in nine hospitals were examined. By conventional typing, only 26 (57.7%) could be assigned to a specific serotype and 6 (13.3%) were autoagglutinating owing to rough lipopolysaccharide antigens. All isolates could be assigned to one of three biotypes, but many phage sensitivity patterns were evident. Twenty-nine distinct strains were identified by combined typing. Probing of EcoRI and BamHI digests of chromosomal DNA with a cDNA copy of E. coli rRNA proved to be highly discriminating between strains. Thirty different ribotypes based on 28 bands were recorded. Overall, agreement between the ribotyping and combined typing methods was good (84.4%), and discrepancies were generally confined to serologically unclassifiable strains and variability in biotype codes. Ribotyping was reproducible, and five of six pairs of isolates from the same and different patients gave identical hybridization profiles on separate occasions. We conclude that ribotyping is a highly discriminatory and reproducible method for the typing of E. cloacae, but in most outbreaks it offers little increase in discrimination over traditional methods.

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Selected References

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  1. Altwegg M., Hickman-Brenner F. W., Farmer J. J., 3rd Ribosomal RNA gene restriction patterns provide increased sensitivity for typing Salmonella typhi strains. J Infect Dis. 1989 Jul;160(1):145–149. doi: 10.1093/infdis/160.1.145. [DOI] [PubMed] [Google Scholar]
  2. Flynn D. M., Weinstein R. A., Nathan C., Gaston M. A., Kabins S. A. Patients' endogenous flora as the source of "nosocomial" Enterobacter in cardiac surgery. J Infect Dis. 1987 Aug;156(2):363–368. doi: 10.1093/infdis/156.2.363. [DOI] [PubMed] [Google Scholar]
  3. Gaston M. A., Bucher C., Pitt T. L. O serotyping scheme for Enterobacter cloacae. J Clin Microbiol. 1983 Nov;18(5):1079–1083. doi: 10.1128/jcm.18.5.1079-1083.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gaston M. A. Enterobacter: an emerging nosocomial pathogen. J Hosp Infect. 1988 Apr;11(3):197–208. doi: 10.1016/0195-6701(88)90098-9. [DOI] [PubMed] [Google Scholar]
  5. Gaston M. A. Evaluation of a bacteriophage-typing scheme for Enterobacter cloacae. J Med Microbiol. 1987 Dec;24(4):291–295. doi: 10.1099/00222615-24-4-291. [DOI] [PubMed] [Google Scholar]
  6. Gaston M. A. Isolation and selection of a bacteriophage-typing set for Enterobacter cloacae. J Med Microbiol. 1987 Dec;24(4):285–290. doi: 10.1099/00222615-24-4-285. [DOI] [PubMed] [Google Scholar]
  7. Grimont F., Grimont P. A. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur Microbiol. 1986 Sep-Oct;137B(2):165–175. doi: 10.1016/s0769-2609(86)80105-3. [DOI] [PubMed] [Google Scholar]
  8. Hitchcock P. J., Brown T. M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983 Apr;154(1):269–277. doi: 10.1128/jb.154.1.269-277.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Irino K., Grimont F., Casin I., Grimont P. A. rRNA gene restriction patterns of Haemophilus influenzae biogroup aegyptius strains associated with Brazilian purpuric fever. J Clin Microbiol. 1988 Aug;26(8):1535–1538. doi: 10.1128/jcm.26.8.1535-1538.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Markowitz S. M., Smith S. M., Williams D. S. Retrospective analysis of plasmid patterns in a study of burn unit outbreaks of infection due to Enterobacter cloacae. J Infect Dis. 1983 Jul;148(1):18–23. doi: 10.1093/infdis/148.1.18. [DOI] [PubMed] [Google Scholar]
  12. Old D. C. Biotyping of Enterobacter cloacae. J Clin Pathol. 1982 Aug;35(8):875–878. doi: 10.1136/jcp.35.8.875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Owen R. J., Beck A., Dayal P. A., Dawson C. Detection of genomic variation in Providencia stuartii clinical isolates by analysis of DNA restriction fragment length polymorphisms containing rRNA cistrons. J Clin Microbiol. 1988 Oct;26(10):2161–2166. doi: 10.1128/jcm.26.10.2161-2166.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Owen R. J. Chromosomal DNA fingerprinting--a new method of species and strain identification applicable to microbial pathogens. J Med Microbiol. 1989 Oct;30(2):89–99. doi: 10.1099/00222615-30-2-89. [DOI] [PubMed] [Google Scholar]
  15. Salvi R. J., Ahroon W., Saunders S. S., Arnold S. A. Evoked potentials: computer-automated threshold-tracking procedure using an objective detection criterion. Ear Hear. 1987 Jun;8(3):151–156. [PubMed] [Google Scholar]
  16. Sanders C. C., Sanders W. E., Jr Microbial resistance to newer generation beta-lactam antibiotics: clinical and laboratory implications. J Infect Dis. 1985 Mar;151(3):399–406. doi: 10.1093/infdis/151.3.399. [DOI] [PubMed] [Google Scholar]
  17. Saunders N. A., Harrison T. G., Kachwalla N., Taylor A. G. Identification of species of the genus Legionella using a cloned rRNA gene from Legionella pneumophila. J Gen Microbiol. 1988 Aug;134(8):2363–2374. doi: 10.1099/00221287-134-8-2363. [DOI] [PubMed] [Google Scholar]
  18. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  19. Stull T. L., LiPuma J. J., Edlind T. D. A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis. 1988 Feb;157(2):280–286. doi: 10.1093/infdis/157.2.280. [DOI] [PubMed] [Google Scholar]
  20. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
  21. Yogev D., Halachmi D., Kenny G. E., Razin S. Distinction of species and strains of mycoplasmas (mollicutes) by genomic DNA fingerprints with an rRNA gene probe. J Clin Microbiol. 1988 Jun;26(6):1198–1201. doi: 10.1128/jcm.26.6.1198-1201.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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