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. 1991 Jul;29(7):1344–1347. doi: 10.1128/jcm.29.7.1344-1347.1991

Cross-reactivity and antigenic heterogeneity among Actinobacillus pleuropneumoniae strains of serotypes 4 and 7.

K R Mittal 1, S Bourdon 1
PMCID: PMC270113  PMID: 1909343

Abstract

Actinobacillus pleuropneumoniae strains of serotypes 4 and 7 were studied for their antigenic properties by means of agglutination, coagglutination, indirect hemagglutination, immunodiffusion, and counterimmunoelectrophoresis tests. Strains of serotype 4 showed cross-reactivity with those of serotype 7 in various serological tests. Serotype 7 strains were antigenically heterogeneous and shared common antigens with several other serotypes. By using boiled whole-cell saline extract as the antigen in the immunodiffusion test, serotype 7 strains could be divided into four subgroups. Subgroup I strains did not have antigens in common with other serotypes, whereas subgroup II strains had antigens in common with serotype 4; subgroup III strains had antigens in common with serotype 10, and subgroup IV had antigens in common with serotypes 1, 9, and 11. The indirect hemagglutination test using unheated whole-cell saline extract as the antigen detected serotype-specific activity. Quantification of serotype-specific and group-specific antigens by coagglutination and immunodiffusion tests was found useful for identifying strains that belonged to serotype 4 or 7.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Biberstein E. L., Gunnarsson A., Hurvell B. Cultural and biochemical criteria for the identification of haemophilus spp from swine. Am J Vet Res. 1977 Jan;38(1):7–11. [PubMed] [Google Scholar]
  2. Cordery M. C., Smith I. M., Mackenzie N. M., Parker D. J. Quantification by flow microfluorimetry of specific binding of antibody to some serotypes of Haemophilus pleuropneumoniae. Res Vet Sci. 1986 Sep;41(2):277–278. [PubMed] [Google Scholar]
  3. Gunnarsson A., Biberstein E. L., Hurvell B. Serologic studies on porcine strains of Haemophilus parahaemolyticus (pleuropneumoniae): agglutination reactions. Am J Vet Res. 1977 Aug;38(8):1111–1114. [PubMed] [Google Scholar]
  4. Hommez J., Devriese L. A., Castryck F., Cassimon P. Slide precipitation: a simple method to type Actinobacillus (Haemophilus) pleuropneumoniae. Vet Microbiol. 1990 Aug;24(2):123–126. doi: 10.1016/0378-1135(90)90059-5. [DOI] [PubMed] [Google Scholar]
  5. Kamp E. M., Popma J. K., Van Leengoed L. A. Serotyping of Haemophilus pleuropneumoniae in the Netherlands: with emphasis on heterogeneity within serotype 1 and (proposed) serotype 9. Vet Microbiol. 1987 Mar;13(3):249–257. doi: 10.1016/0378-1135(87)90087-3. [DOI] [PubMed] [Google Scholar]
  6. Mittal K. R., Higgins R., Lariviere S. Determination of antigenic specificity and relationship among Haemophilus pleuropneumoniae serotypes by an indirect hemagglutination test. J Clin Microbiol. 1983 May;17(5):787–790. doi: 10.1128/jcm.17.5.787-790.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mittal K. R., Higgins R., Lariviere S. Evaluation of slide agglutination and ring precipitation tests for capsular serotyping of Haemophilus pleuropneumoniae. J Clin Microbiol. 1982 Jun;15(6):1019–1023. doi: 10.1128/jcm.15.6.1019-1023.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mittal K. R., Higgins R., Lariviere S. Quantitation of serotype-specific and cross-reacting group-specific antigens by coagglutination and immunodiffusion tests for differentiating Actinobacillus (Haemophilus) pleuropneumoniae strains belonging to cross-reacting serotypes 3, 6, and 8. J Clin Microbiol. 1988 May;26(5):985–989. doi: 10.1128/jcm.26.5.985-989.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mittal K. R., Higgins R., Larivière S. Identification and serotyping of Haemophilus pleuropneumoniae by coagglutination test. J Clin Microbiol. 1983 Dec;18(6):1351–1354. doi: 10.1128/jcm.18.6.1351-1354.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mittal K. R., Higgins R., Larivière S., Leblanc D. A 2-mercaptoethanol tube agglutination test for diagnosis of Haemophilus pleuropneumoniae infection in pigs. Am J Vet Res. 1984 Apr;45(4):715–719. [PubMed] [Google Scholar]
  11. Nicolet J. Sur l'hémophilose du pore. 3. Différenciation sérologique de Haemophilus parahaemolyticus. Zentralbl Bakteriol Orig. 1971;216(4):487–495. [PubMed] [Google Scholar]
  12. Nicolet J. Taxonomy and Serological Identification of Actinobacillus pleuropneumoniae. Can Vet J. 1988 Jul;29(7):578–580. [PMC free article] [PubMed] [Google Scholar]
  13. Nielsen R. Seroepidemiology of Actinobacillus pleuropneumoniae. Can Vet J. 1988 Jul;29(7):580–582. [PMC free article] [PubMed] [Google Scholar]
  14. Nielsen R. Serological characterization of Actinobacillus pleuropneumoniae strains and proposal of a new serotype: serotype 12. Acta Vet Scand. 1986;27(3):453–455. doi: 10.1186/BF03548158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Piffer I. A., Carter G. R., Botovchenco A. A. Identification of serotypes of Haemophilus pleuropneumoniae by counterimmunoelectrophoresis. Vet Rec. 1986 Mar 15;118(11):292–294. doi: 10.1136/vr.118.11.292. [DOI] [PubMed] [Google Scholar]
  16. Rapp V. J., Ross R. F., Erickson B. Z. Serotyping of Haemophilus pleuropneumoniae by rapid slide agglutination and indirect fluorescent antibody tests in swine. Am J Vet Res. 1985 Jan;46(1):185–192. [PubMed] [Google Scholar]
  17. Rosendal S., Boyd D. A. Haemophilus pleuropneumoniae serotyping. J Clin Microbiol. 1982 Nov;16(5):840–843. doi: 10.1128/jcm.16.5.840-843.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sebunya T. N., Saunders J. R. Haemophilus pleuropneumoniae infection in swine: a review. J Am Vet Med Assoc. 1983 Jun 15;182(12):1331–1337. [PubMed] [Google Scholar]

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