Abstract
Restriction fragment length polymorphism analysis was performed with the endonucleases EcoRI, BglII, and HinfI on a collection of Candida albicans strains comprising eight strains randomly selected from clinical microbiology laboratory specimens, three reported azole-resistant strains from treatment failures, and several subcultures of the azole-resistant strain NCPF 3310 (also known as the Darlington strain) received from different laboratories. The results demonstrated a diversity of the restriction fragment length polymorphism patterns that were obtained and revealed that two of the proposed Darlington subcultures had patterns distinct from each other and from those of the other Darlington isolates; both were also found to have lost their azole resistance.
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- Hitchcock C. A., Barrett-Bee K. J., Russell N. J. Inhibition of 14 alpha-sterol demethylase activity in Candida albicans Darlington does not correlate with resistance to azole. J Med Vet Mycol. 1987 Oct;25(5):329–333. [PubMed] [Google Scholar]
- Hitchcock C. A., Barrett-Bee K. J., Russell N. J. The lipid composition of azole-sensitive and azole-resistant strains of Candida albicans. J Gen Microbiol. 1986 Sep;132(9):2421–2431. doi: 10.1099/00221287-132-9-2421. [DOI] [PubMed] [Google Scholar]
- Howell S. A., Mallet A. I., Noble W. C. A comparison of the sterol content of multiple isolates of the Candida albicans Darlington strain with other clinically azole-sensitive and -resistant strains. J Appl Bacteriol. 1990 Nov;69(5):692–696. doi: 10.1111/j.1365-2672.1990.tb01564.x. [DOI] [PubMed] [Google Scholar]
- Matthews R., Burnie J. Assessment of DNA fingerprinting for rapid identification of outbreaks of systemic candidiasis. BMJ. 1989 Feb 11;298(6670):354–357. doi: 10.1136/bmj.298.6670.354. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Odds F. C., Webster C. E., Fisk P. G., Riley V. C., Mayuranathan P., Simmons P. D. Candida species and C. albicans biotypes in women attending clinics in genitourinary medicine. J Med Microbiol. 1989 May;29(1):51–54. doi: 10.1099/00222615-29-1-51. [DOI] [PubMed] [Google Scholar]
- Phongpaichit S., Mackenzie D. W., Fraser C. Strain differentiation of Candida albicans by morphotyping. Epidemiol Infect. 1987 Oct;99(2):421–428. doi: 10.1017/s0950268800067911. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith R. A., Hitchcock C. A., Evans E. G., Lacey C. J., Adams D. J. The identification of Candida albicans strains by restriction fragment length polymorphism analysis of DNA. J Med Vet Mycol. 1989;27(6):431–434. [PubMed] [Google Scholar]
- Warnock D. W., Johnson E. M., Richardson M. D., Vickers C. F. Modified response to ketoconazole of Candida albicans from a treatment failure. Lancet. 1983 Mar 19;1(8325):642–643. doi: 10.1016/s0140-6736(83)91809-3. [DOI] [PubMed] [Google Scholar]
- Warnock D. W., Speller C. D., Milne J. D., Hilton A. L., Kershaw P. I. Epidemiological investigation of patients with vulvovaginal candidosis. Application of a resistogram method for strain differentiation of Candida albicans. Br J Vener Dis. 1979 Oct;55(5):357–361. doi: 10.1136/sti.55.5.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wills J. W., Lasker B. A., Sirotkin K., Riggsby W. S. Repetitive DNA of Candida albicans: nuclear and mitochondrial components. J Bacteriol. 1984 Mar;157(3):918–924. doi: 10.1128/jb.157.3.918-924.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]