Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1991 Jul;29(7):1368–1371. doi: 10.1128/jcm.29.7.1368-1371.1991

Prospective multicenter study on isolation of human immunodeficiency virus type 1 from homosexual men after seroconversion.

P Gupta 1, H Farzadegan 1, D Imagawa 1, M Lee 1, L Kingsley 1, S Zhou 1, J Armstrong 1, C Rinaldo 1
PMCID: PMC270119  PMID: 1885732

Abstract

A prospective multicenter study was undertaken to isolate human immunodeficiency virus type 1 (HIV-1) from 45 homosexual men for a period of 30 months after seroconversion. Efficiency of HIV-1 isolation from peripheral blood mononuclear cells was relatively stable over time, ranging from 64% at the time of seroconversion to more than 82% after 18 months of seroconversion. However, Kaplan-Meier analysis of HIV-1 culture data indicates that the cumulative proportion of HIV-1 culture positivity at 3, 6, 12, and 18 months after seroconversion was 62, 65, 84, and 92%, respectively. No significant correlation was observed between the presence of HIV-1 p24 antigen in serum, or numbers of CD4+ and CD8+ blood lymphocytes, and HIV-1 isolation within this period of time. These data suggest that HIV-1 viremia in homosexual men gradually increases to almost 100% culture positivity by 18 months after seroconversion.

Full text

PDF
1368

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allain J. P., Laurian Y., Paul D. A., Senn D. Serological markers in early stages of human immunodeficiency virus infection in haemophiliacs. Lancet. 1986 Nov 29;2(8518):1233–1236. doi: 10.1016/s0140-6736(86)92673-5. [DOI] [PubMed] [Google Scholar]
  2. Balachandran R., Thampatty P., Rinaldo C., Gupta P. Use of cryopreserved normal peripheral blood lymphocytes for isolation of human immunodeficiency virus from seropositive men. J Clin Microbiol. 1988 Mar;26(3):595–597. doi: 10.1128/jcm.26.3.595-597.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brinchmann J. E., Gaudernack G., Vartdal F. CD8+ T cells inhibit HIV replication in naturally infected CD4+ T cells. Evidence for a soluble inhibitor. J Immunol. 1990 Apr 15;144(8):2961–2966. [PubMed] [Google Scholar]
  4. Fauci A. S. The human immunodeficiency virus: infectivity and mechanisms of pathogenesis. Science. 1988 Feb 5;239(4840):617–622. doi: 10.1126/science.3277274. [DOI] [PubMed] [Google Scholar]
  5. Gendelman H. E., Orenstein J. M., Martin M. A., Ferrua C., Mitra R., Phipps T., Wahl L. A., Lane H. C., Fauci A. S., Burke D. S. Efficient isolation and propagation of human immunodeficiency virus on recombinant colony-stimulating factor 1-treated monocytes. J Exp Med. 1988 Apr 1;167(4):1428–1441. doi: 10.1084/jem.167.4.1428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Giorgi J. V., Cheng H. L., Margolick J. B., Bauer K. D., Ferbas J., Waxdal M., Schmid I., Hultin L. E., Jackson A. L., Park L. Quality control in the flow cytometric measurement of T-lymphocyte subsets: the multicenter AIDS cohort study experience. The Multicenter AIDS Cohort Study Group. Clin Immunol Immunopathol. 1990 May;55(2):173–186. doi: 10.1016/0090-1229(90)90096-9. [DOI] [PubMed] [Google Scholar]
  7. Gupta P., Enrico A., Armstrong J., Doerr M., Ho M., Rinaldo C. A semiquantitative microassay for measurement of relative number of blood mononuclear cells infected with human immunodeficiency virus. AIDS Res Hum Retroviruses. 1990 Oct;6(10):1193–1196. doi: 10.1089/aid.1990.6.1193. [DOI] [PubMed] [Google Scholar]
  8. Ho D. D., Moudgil T., Alam M. Quantitation of human immunodeficiency virus type 1 in the blood of infected persons. N Engl J Med. 1989 Dec 14;321(24):1621–1625. doi: 10.1056/NEJM198912143212401. [DOI] [PubMed] [Google Scholar]
  9. Jackson J. B., Kwok S. Y., Sninsky J. J., Hopsicker J. S., Sannerud K. J., Rhame F. S., Henry K., Simpson M., Balfour H. H., Jr Human immunodeficiency virus type 1 detected in all seropositive symptomatic and asymptomatic individuals. J Clin Microbiol. 1990 Jan;28(1):16–19. doi: 10.1128/jcm.28.1.16-19.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lee M. H., Sano K., Morales F. E., Imagawa D. T. Sensitive reverse transcriptase assay to detect and quantitate human immunodeficiency virus. J Clin Microbiol. 1987 Sep;25(9):1717–1721. doi: 10.1128/jcm.25.9.1717-1721.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Muñoz A., Wang M. C., Bass S., Taylor J. M., Kingsley L. A., Chmiel J. S., Polk B. F. Acquired immunodeficiency syndrome (AIDS)-free time after human immunodeficiency virus type 1 (HIV-1) seroconversion in homosexual men. Multicenter AIDS Cohort Study Group. Am J Epidemiol. 1989 Sep;130(3):530–539. doi: 10.1093/oxfordjournals.aje.a115367. [DOI] [PubMed] [Google Scholar]
  12. Nicholson J. K., Spira T. J., Aloisio C. H., Jones B. M., Kennedy M. S., Holman R. C., McDougal J. S. Serial determinations of HIV-1 titers in HIV-infected homosexual men: association of rising titers with CD4 T cell depletion and progression to AIDS. AIDS Res Hum Retroviruses. 1989 Apr;5(2):205–215. doi: 10.1089/aid.1989.5.205. [DOI] [PubMed] [Google Scholar]
  13. Owen A. J., Pantazis P., Antoniades H. N. Simian sarcoma virus--transformed cells secrete a mitogen identical to platelet-derived growth factor. Science. 1984 Jul 6;225(4657):54–56. doi: 10.1126/science.6328659. [DOI] [PubMed] [Google Scholar]
  14. Psallidopoulos M. C., Schnittman S. M., Thompson L. M., 3rd, Baseler M., Fauci A. S., Lane H. C., Salzman N. P. Integrated proviral human immunodeficiency virus type 1 is present in CD4+ peripheral blood lymphocytes in healthy seropositive individuals. J Virol. 1989 Nov;63(11):4626–4631. doi: 10.1128/jvi.63.11.4626-4631.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rinaldo C., Kingsley L., Neumann J., Reed D., Gupta P., Lyter D. Association of human immunodeficiency virus (HIV) p24 antigenemia with decrease in CD4+ lymphocytes and onset of acquired immunodeficiency syndrome during the early phase of HIV infection. J Clin Microbiol. 1989 May;27(5):880–884. doi: 10.1128/jcm.27.5.880-884.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schnittman S. M., Psallidopoulos M. C., Lane H. C., Thompson L., Baseler M., Massari F., Fox C. H., Salzman N. P., Fauci A. S. The reservoir for HIV-1 in human peripheral blood is a T cell that maintains expression of CD4. Science. 1989 Jul 21;245(4915):305–308. doi: 10.1126/science.2665081. [DOI] [PubMed] [Google Scholar]
  17. Simmonds P., Balfe P., Peutherer J. F., Ludlam C. A., Bishop J. O., Brown A. J. Human immunodeficiency virus-infected individuals contain provirus in small numbers of peripheral mononuclear cells and at low copy numbers. J Virol. 1990 Feb;64(2):864–872. doi: 10.1128/jvi.64.2.864-872.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wolinsky S. M., Rinaldo C. R., Kwok S., Sninsky J. J., Gupta P., Imagawa D., Farzadegan H., Jacobson L. P., Grovit K. S., Lee M. H. Human immunodeficiency virus type 1 (HIV-1) infection a median of 18 months before a diagnostic western blot. Evidence from a cohort of homosexual men. Ann Intern Med. 1989 Dec 15;111(12):961–972. doi: 10.7326/0003-4819-111-12-961. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES