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. 1991 Jul;29(7):1523–1526. doi: 10.1128/jcm.29.7.1523-1526.1991

Detection of the Lyme disease bacterium, Borrelia burgdorferi, by using the polymerase chain reaction and a nonradioisotopic gene probe.

D J Wise 1, T L Weaver 1
PMCID: PMC270147  PMID: 1885749

Abstract

A 419-bp region of the flagellin gene sequence of Borrelia burgdorferi was used as a target for the polymerase chain reaction. With a nonradioactively labeled gene-specific probe, sensitivity to as few as 1 to 10 spirochetes was observed. The targeted gene fragment was conserved in the American and European strains of B. burgdorferi tested and among several other pathogenic borreliae.

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Selected References

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