Abstract
Leptospira interrogans serovar icterohaemorrhagiae RGA (RGA), live or heated at 56 degrees C for 15 min or treated with Formalin, was injected into rabbits to prepare hyperimmune serum. The pathogens L. interrogans serovars icterohaemorrhagiae RGA, icterohaemorrhagiae 1, canicola Moulton, grippotyphosa Andaman, hardjo Hardjoprajitno, and pomona Pomona and the nonpathogen Leptospina biflexa serovar patoc Patoc 1 were processed for sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and after electrophoresis they were then transferred to nitrocellulose paper. Antiserum against RGA (live, heat killed, or Formalin killed) was used on one of each of the three blots. Formalin appeared to completely eliminate antibody response to antigens with the molecular weight of 14,000 to 20,000 (14K to 20K) but did expose an antigen at approximately 23K in the pathogens only. This same band had only slight reactivity when antiserum against heat-killed RGA was used. Heating also eliminated cross-reactivity in the 19K to 30K range and partially degraded bands in the 14K to 20K region so that one broad band rather than several discrete bands appeared. The three antiserum specimens cross-reacted with all of the serovars tested, but fewer antigens of grippotyphosa and hardjo reacted with the antisera. Against patoc, reactivity was limited primarily to the flagellar region. The most cross-reactivity was with the antiserum prepared by using live leptospires.
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- Babudieri B., Castelli M., Pisoni F. Comparative tests with formolized and irradiated vaccines against leptospirosis. Bull World Health Organ. 1973 May;48(5):587–590. [PMC free article] [PubMed] [Google Scholar]
- Bey R. F., Johnson R. C. Current status of leptospiral vaccines. Prog Vet Microbiol Immunol. 1986;2:175–197. [PubMed] [Google Scholar]
- Chapman A. J., Adler B., Faine S. Antigens recognised by the human immune response to infection with Leptospira interrogans serovar hardjo. J Med Microbiol. 1988 Apr;25(4):269–278. doi: 10.1099/00222615-25-4-269. [DOI] [PubMed] [Google Scholar]
- Cinco M., Banfi E., Panfili E. Leptospiral lipopolysaccharide presence in the outer envelope: electrophoretic evidence and immunological specificity. Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Nov;269(3):277–283. doi: 10.1016/s0176-6724(88)80171-8. [DOI] [PubMed] [Google Scholar]
- Hodges R. T. Leptospira interrogans serotype pomona infection in pigs: prevention of leptospiruria by immunisation before exposure to a natural infection. N Z Vet J. 1977 Jan-Feb;25(1-2):33–35. doi: 10.1080/00480169.1977.34346. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Manev C. Serological characteristics of the Leptospira serogroup Pomona. II. Changes in the agglutination and absorption properties of the reference strains after formalin- and heat-inactivation. Zentralbl Bakteriol Orig A. 1976 Nov;236(2-3):323–327. [PubMed] [Google Scholar]
- Painter G. M., Ellinghausen H. C., Jr Immunising potency of Leptospira interrogans serotype canicola after heat inactivation at different temperatures. J Med Microbiol. 1976 Nov;9(4):487–492. doi: 10.1099/00222615-9-4-487. [DOI] [PubMed] [Google Scholar]
- Palmer M. F., Waitkins S. A., Wanyangu S. W. A comparison of live and formalised leptospiral microscopic agglutination test. Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Jun;265(1-2):151–159. doi: 10.1016/s0176-6724(87)80161-x. [DOI] [PubMed] [Google Scholar]
- Pope V., Johnson R. C. Effect of heat or chemical treatment on leptospiral antigens. J Clin Microbiol. 1987 Feb;25(2):255–258. doi: 10.1128/jcm.25.2.255-258.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]