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. 1991 Aug;29(8):1596–1603. doi: 10.1128/jcm.29.8.1596-1603.1991

Application of colorimetric microdilution plate hybridization for rapid genetic identification of 22 Mycobacterium species.

S Kusunoki 1, T Ezaki 1, M Tamesada 1, Y Hatanaka 1, K Asano 1, Y Hashimoto 1, E Yabuuchi 1
PMCID: PMC270169  PMID: 1761680

Abstract

Quantitative microdilution plate hybridization was used to identify 22 Mycobacterium species. DNAs of clinical strains were rapidly extracted and labeled with photoreactive biotin. Labeled DNAs were distributed into wells of a microdilution plate in which reference DNAs had been immobilized. After 2 h of hybridization, hybridized DNAs were quantitatively detected with peroxidase-conjugated streptavidin and the substrate, tetramethylbenzidine. This method could differentiate among 20 of the 22 Mycobacterium species tested. The type strains of Mycobacterium tuberculosis and M. bovis were genetically highly related and could not be differentiated by this method. Of 194 biochemically identified human clinical strains, 178 (90%) were genetically identified within 3 h of the small-scale DNA extraction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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