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. 1991 Aug;29(8):1616–1619. doi: 10.1128/jcm.29.8.1616-1619.1991

Comparison of the PREMIER cryptococcal antigen enzyme immunoassay and the latex agglutination assay for detection of cryptococcal antigens.

W Gade 1, S W Hinnefeld 1, L S Babcock 1, P Gilligan 1, W Kelly 1, K Wait 1, D Greer 1, M Pinilla 1, R L Kaplan 1
PMCID: PMC270172  PMID: 1761681

Abstract

A new enzyme immunoassay (EIA), PREMIER Cryptococcal Antigen, was compared with latex agglutination (LA) for the detection and quantitation of circulating capsular polysaccharide antigen from Cryptococcus neoformans. The clinical evaluation of PREMIER EIA as a screening assay, including 475 specimens with 120 LA and EIA positives, resulted in 99% sensitivity and 97% specificity. The clinical specimens included sera and cerebrospinal fluids as well as 10 rheumatoid factor-positive and 20 anti-nuclear antibody-positive serum samples. This monoclonal antibody-based assay detects serotypes A to D at 0.63, 0.63, 7.8, and 62 ng/ml, respectively. With three different known positive specimens, the assay was found to yield coefficients of variation of 2 to 12% for intra- and interassay comparisons of precision and reproducibility. The primary use for semiquantitative values derived with the LA or EIA is to follow the course of disease and monitor drug therapies. The present data suggest that the PREMIER EIA will be a valuable method for this purpose. We conclude that the PREMIER Cryptococcal Antigen EIA provides a rapid, convenient, and reliable antigen detection method for screening and semiquantitative determination of antigen levels.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENNETT J. E., HASENCLEVER H. F., TYNES B. S. DETECTION OF CRYPTOCOCCAL POLYSACCHARIDE IN SERUM AND SPINAL FLUID: VALUE IN DIAGNOSIS AND PROGNOSIS. Trans Assoc Am Physicians. 1964;77:145–150. [PubMed] [Google Scholar]
  2. Bottone E. J., Salkin I. F., Hurd N. J., Wormser G. P. Serogroup distribution of Cryptococcus neoformans in patients with AIDS. J Infect Dis. 1987 Jul;156(1):242–242. doi: 10.1093/infdis/156.1.242. [DOI] [PubMed] [Google Scholar]
  3. Diamond R. D., Bennett J. E. Prognostic factors in cryptococcal meningitis. A study in 111 cases. Ann Intern Med. 1974 Feb;80(2):176–181. doi: 10.7326/0003-4819-80-2-176. [DOI] [PubMed] [Google Scholar]
  4. Eckert T. F., Kozel T. R. Production and characterization of monoclonal antibodies specific for Cryptococcus neoformans capsular polysaccharide. Infect Immun. 1987 Aug;55(8):1895–1899. doi: 10.1128/iai.55.8.1895-1899.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goodman J. S., Kaufman L., Koenig M. G. Diagnosis of cryptococcal meningitis. Value of immunologic detection of cryptococcal antigen. N Engl J Med. 1971 Aug 19;285(8):434–436. doi: 10.1056/NEJM197108192850804. [DOI] [PubMed] [Google Scholar]
  6. Gray L. D., Roberts G. D. Experience with the use of pronase to eliminate interference factors in the latex agglutination test for cryptococcal antigen. J Clin Microbiol. 1988 Nov;26(11):2450–2451. doi: 10.1128/jcm.26.11.2450-2451.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McManus E. J., Jones J. M. Detection of a Trichosporon beigelii antigen cross-reactive with Cryptococcus neoformans capsular polysaccharide in serum from a patient with disseminated Trichosporon infection. J Clin Microbiol. 1985 May;21(5):681–685. doi: 10.1128/jcm.21.5.681-685.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Melcher G. P., Reed K. D., Rinaldi M. G., Lee J. W., Pizzo P. A., Walsh T. J. Demonstration of a cell wall antigen cross-reacting with cryptococcal polysaccharide in experimental disseminated trichosporonosis. J Clin Microbiol. 1991 Jan;29(1):192–196. doi: 10.1128/jcm.29.1.192-196.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Murphy J. W., Mosley R. L., Cherniak R., Reyes G. H., Kozel T. R., Reiss E. Serological, electrophoretic, and biological properties of Cryptococcus neoformans antigens. Infect Immun. 1988 Feb;56(2):424–431. doi: 10.1128/iai.56.2.424-431.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Patterson T. F., Andriole V. T. Current concepts in cryptococcosis. Eur J Clin Microbiol Infect Dis. 1989 May;8(5):457–465. doi: 10.1007/BF01964060. [DOI] [PubMed] [Google Scholar]
  11. Perfect J. R. Cryptococcosis. Infect Dis Clin North Am. 1989 Mar;3(1):77–102. [PubMed] [Google Scholar]

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