Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1991 Aug;29(8):1652–1658. doi: 10.1128/jcm.29.8.1652-1658.1991

Mycobacterium paratuberculosis antigen D: characterization and evidence that it is a bacterioferritin.

B W Brooks 1, N M Young 1, D C Watson 1, R H Robertson 1, E A Sugden 1, K H Nielsen 1, S A Becker 1
PMCID: PMC270179  PMID: 1761687

Abstract

By using a combination of agarose and polyacrylamide gel electrophoresis, Mycobacterium paratuberculosis antigen D was resolved from a crude sonicated preparation of the organism and characterized as a component with a molecular mass of approximately 400,000 Da. While this component was composed mainly of protein, with unusually high proportions of glutamic acid and leucine, it was resistant to digestion with a number of proteolytic enzymes. Structural detail revealed by electron microscopy, amino acid sequence data, and the demonstration of a Soret band in its absorption spectrum indicated that antigen D was similar to an Escherichia coli bacterioferritin.

Full text

PDF
1652

Images in this article

1654Image
on p.1654
1654Image
on p.1654
1655Image
on p.1655
1656Image
on p.1656

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews S. C., Smith J. M., Guest J. R., Harrison P. M. Amino acid sequence of the bacterioferritin (cytochrome b1) of Escherichia coli-K12. Biochem Biophys Res Commun. 1989 Jan 31;158(2):489–496. doi: 10.1016/s0006-291x(89)80075-0. [DOI] [PubMed] [Google Scholar]
  2. Banyard S. H., Stammers D. K., Harrison P. M. Electron density map of apoferritin at 2.8-A resolution. Nature. 1978 Jan 19;271(5642):282–284. doi: 10.1038/271282a0. [DOI] [PubMed] [Google Scholar]
  3. Bech-Nielsen S., Burianek L. L., Spangler E., Heider L. E., Hoffsis G. F., Dorn C. R. Characterization of Mycobacterium paratuberculosis antigenic proteins. Am J Vet Res. 1985 Nov;46(11):2418–2420. [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  5. Brooks B. W., Robertson R. H., Corner A. H., Samagh B. S., Garcia M. M., Turcotte C., Duncan J. R. Evaluation of the serological response of sheep in one flock to Mycobacterium paratuberculosis by crossed immunoelectrophoresis. Can J Vet Res. 1988 Apr;52(2):199–204. [PMC free article] [PubMed] [Google Scholar]
  6. Bulen W. A., LeComte J. R., Lough S. A hemoprotein from azotobacter containing non-heme iron: isolation and crystallization. Biochem Biophys Res Commun. 1973 Oct 15;54(4):1274–1281. doi: 10.1016/0006-291x(73)91125-x. [DOI] [PubMed] [Google Scholar]
  7. Chiodini R. J., Van Kruiningen H. J., Merkal R. S. Ruminant paratuberculosis (Johne's disease): the current status and future prospects. Cornell Vet. 1984 Jul;74(3):218–262. [PubMed] [Google Scholar]
  8. Duncan J. R., Hall C. E., de Lisle C. Johne's disease and the practitioner. Cornell Vet. 1978 Jan;68 (Suppl 7):179–188. [PubMed] [Google Scholar]
  9. Gruft H., Gaafar H. A. Multiple catalases of mycobacteria: differences in molecular weight. Am Rev Respir Dis. 1974 Sep;110(3):320–323. doi: 10.1164/arrd.1974.110.3.320. [DOI] [PubMed] [Google Scholar]
  10. Hunter S. W., McNeil M., Modlin R. L., Mehra V., Bloom B. R., Brennan P. J. Isolation and characterization of the highly immunogenic cell wall-associated protein of Mycobacterium leprae. J Immunol. 1989 Apr 15;142(8):2864–2872. [PubMed] [Google Scholar]
  11. Hunter S. W., Rivoire B., Mehra V., Bloom B. R., Brennan P. J. The major native proteins of the leprosy bacillus. J Biol Chem. 1990 Aug 25;265(24):14065–14068. [PubMed] [Google Scholar]
  12. Kadir F. H., Moore G. R. Bacterial ferritin contains 24 haem groups. FEBS Lett. 1990 Oct 1;271(1-2):141–143. doi: 10.1016/0014-5793(90)80391-u. [DOI] [PubMed] [Google Scholar]
  13. Kroll J. Tandem-crossed immunoelectrophoresis. Scand J Immunol Suppl. 1973;1:57–59. [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Lenzini L., Rottoli P., Rottoli L. The spectrum of human tuberculosis. Clin Exp Immunol. 1977 Feb;27(2):230–237. [PMC free article] [PubMed] [Google Scholar]
  16. Lygren S. T., Closs O., Bercouvier H., Wayne L. G. Catalases, peroxidases, and superoxide dismutases in Mycobacterium leprae and other mycobacteria studied by crossed immunoelectrophoresis and polyacrylamide gel electrophoresis. Infect Immun. 1986 Dec;54(3):666–672. doi: 10.1128/iai.54.3.666-672.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Milner A. R., Lepper A. W., Symonds W. N., Gruner E. Analysis by ELISA and Western blotting of antibody reactivities in cattle infected with Mycobacterium paratuberculosis after absorption of serum with M phlei. Res Vet Sci. 1987 Mar;42(2):140–144. [PubMed] [Google Scholar]
  18. Myrvang B., Feek C. M., Godal T. Antimycobacterial antibodies in sera from patients throughout the clinico-pathological disease spectrum of leprosy. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Oct;82B(5):701–706. doi: 10.1111/j.1699-0463.1974.tb00238.x. [DOI] [PubMed] [Google Scholar]
  19. Riemann H. P., Abbas B. Diagnosis and control of bovine paratuberculosis (Johne's disease). Adv Vet Sci Comp Med. 1983;27:481–506. [PubMed] [Google Scholar]
  20. Shinnick T. M., Sweetser D., Thole J., van Embden J., Young R. A. The etiologic agents of leprosy and tuberculosis share an immunoreactive protein antigen with the vaccine strain Mycobacterium bovis BCG. Infect Immun. 1987 Aug;55(8):1932–1935. doi: 10.1128/iai.55.8.1932-1935.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Smith J. M., Quirk A. V., Plank R. W., Diffin F. M., Ford G. C., Harrison P. M. The identity of Escherichia coli bacterioferritin and cytochrome b1. Biochem J. 1988 Oct 15;255(2):737–740. [PMC free article] [PubMed] [Google Scholar]
  22. Stiefel E. I., Watt G. D. Azotobacter cytochrome b557.5 is a bacterioferritin. Nature. 1979 May 3;279(5708):81–83. doi: 10.1038/279081a0. [DOI] [PubMed] [Google Scholar]
  23. Sugden E. A., Brooks B. W., Young N. M., Watson D. C., Nielsen K. H., Corner A. H., Turcotte C., Michaelides A., Stewart R. B. Chromatographic purification and characterization of antigens A and D from Mycobacterium paratuberculosis and their use in enzyme-linked immunosorbent assays for diagnosis of paratuberculosis in sheep. J Clin Microbiol. 1991 Aug;29(8):1659–1664. doi: 10.1128/jcm.29.8.1659-1664.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sugden E. A., Samagh B. S., Bundle D. R., Duncan J. R. Lipoarabinomannan and lipid-free arabinomannan antigens of Mycobacterium paratuberculosis. Infect Immun. 1987 Mar;55(3):762–770. doi: 10.1128/iai.55.3.762-770.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
  26. Tsugita A., Yariv J. Preliminary results for the primary structure of bacterioferritin of Escherichia coli. Biochem J. 1985 Oct 1;231(1):209–212. doi: 10.1042/bj2310209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Yamamura Y., Misaki A., Azuma I. Chemical and immunological studies on polysaccharide antigens of mycobacteria, nocardia and corynebacteria. Bull Int Union Tuberc. 1972 Feb;47:181–191. [PubMed] [Google Scholar]
  28. Yariv J., Kalb A. J., Sperling R., Bauminger E. R., Cohen S. G., Ofer S. The composition and the structure of bacterioferritin of Escherichia coli. Biochem J. 1981 Jul 1;197(1):171–175. doi: 10.1042/bj1970171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yariv J. The identity of bacterioferritin and cytochrome b1. Biochem J. 1983 May 1;211(2):527–527. doi: 10.1042/bj2110527. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES