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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1991 Aug;29(8):1752–1753. doi: 10.1128/jcm.29.8.1752-1753.1991

Detection of rubella-specific immunoglobulin G: comparison of the enzyme-linked immunosorbent assay and an automated microparticle enzyme immunoassay (IMx).

I J Skurrie 1, J L Head 1, S M Garland 1
PMCID: PMC270199  PMID: 1761701

Abstract

An automated microparticle enzyme immunoassay (IMx Rubella IgG Antibody Assay; Abbott Laboratories, North Chicago, Ill.) was compared with a conventional enzyme-linked immunosorbent assay (ELISA) for detection of rubella-specific immunoglobulin G (IgG) in 400 consecutive antenatal patients. There was complete agreement between the two tests in this population, which had a positivity rate of 99% for rubella-specific IgG antibodies. The performance of the IMx was also evaluated at the cutoff zone by assaying 64 selected antenatal serum samples with low or negative rubella antibody titers as determined by ELISA. Overall, the IMx was found to be a specific, sensitive assay for the detection of rubella-specific IgG and is virtually fully automated for easy performance.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Field P. R., Ho D. W., Cunningham A. L. Evaluation of rubella immune status by three commercial enzyme-linked immunosorbent assays. J Clin Microbiol. 1988 May;26(5):990–994. doi: 10.1128/jcm.26.5.990-994.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Haukenes G., Blom H. False positive rubella virus haemagglutination inhibition reactions: occurrence and disclosure. Med Microbiol Immunol. 1975;161(2):99–106. doi: 10.1007/BF02121750. [DOI] [PubMed] [Google Scholar]
  3. Herrmann K. L. Available rubella serologic tests. Rev Infect Dis. 1985 Mar-Apr;7 (Suppl 1):S108–S112. doi: 10.1093/clinids/7.supplement_1.s108. [DOI] [PubMed] [Google Scholar]
  4. Schaefer L. E., Dyke J. W., Meglio F. D., Murray P. R., Crafts W., Niles A. C. Evaluation of microparticle enzyme immunoassays for immunoglobulins G and M to rubella virus and Toxoplasma gondii on the Abbott IMx automated analyzer. J Clin Microbiol. 1989 Nov;27(11):2410–2413. doi: 10.1128/jcm.27.11.2410-2413.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Schmidt N. J., Dennis J. Modified hemagglutination-inhibition test for rubella employing human group O erythrocytes. Appl Microbiol. 1972 Mar;23(3):471–475. doi: 10.1128/am.23.3.471-475.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Skurrie I. J., Gilbert G. L. Enzyme-linked immunosorbent assay for rubella immunoglobulin G: new method for attachment of antigens to microtiter plates. J Clin Microbiol. 1983 May;17(5):738–743. doi: 10.1128/jcm.17.5.738-743.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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