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. 1991 Sep;29(9):1868–1872. doi: 10.1128/jcm.29.9.1868-1872.1991

Human cytomegalovirus structural proteins: immune reaction against pp150 synthetic peptides.

M P Landini 1, A Ripalti 1, K Sra 1, P Pouletty 1
PMCID: PMC270226  PMID: 1723077

Abstract

In the present study, several peptides of the major structural antigen (pp150) of human cytomegalovirus (CMV) have been chemically synthesized and tested by a modified slot blotting procedure for their ability to bind CMV-specific immunoglobulin G (IgG) and IgM present in human sera. The sequences of the peptides were deduced on the basis of either (i) their presence in a fusion protein already known to be frequently recognized by human antibody or (ii) their high content of hydrophilic amino acids as deduced from the published nucleotide sequence. An important IgM-binding epitope was found to be located in the last 38 amino acids at the carboxy terminus of the molecule. This region reacts with anti-CMV IgM present in the great majority (83.3%) of IgM-positive human sera, and adsorption experiments have shown that IgM titers to the entire pp150 decrease 25 to 50% in most sera previously absorbed with this region. The overall results obtained endorse the continued synthesis of other sequences in order to define a group of peptides sensitive and specific enough to replace the virus and infected cells as an antigenic substrate in the serological evaluation of anti-CMV antibody.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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