Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1991 Sep;29(9):1949–1954. doi: 10.1128/jcm.29.9.1949-1954.1991

Molecular characterization of Haemophilus ducreyi by ribosomal DNA fingerprinting.

S K Sarafian 1, T C Woods 1, J S Knapp 1, B Swaminathan 1, S A Morse 1
PMCID: PMC270240  PMID: 1663518

Abstract

Intraspecies genotypic heterogeneity among Haemophilus ducreyi isolates was examined by using genomic fingerprints with rRNA from Escherichia coli as a probe. DNA from 44 isolates of H. ducreyi was digested by restriction endonuclease HincII or HindIII, separated by agarose gel electrophoresis, transferred to nylon membranes, and hybridized with 32P-labeled 16S and 23S rRNA. HincII digests yielded four hybridization patterns (ribotypes), whereas HindIII digests yielded eight ribotypes. Four HincII and five HindIII ribotypes were observed among 14 H. ducreyi isolates collected within a period of 1 month in Kenya, where chancroid is endemic. In contrast, one HincII and two HindIII ribotypes were observed among 28 isolates collected during the Orange County, Calif., chancroid epidemic that occurred in 1981 and 1982. The plasmid content, in conjunction with ribotyping, provided additional differentiation among some isolates of H. ducreyi. This study demonstrates that ribotyping of H. ducreyi may be used to study the epidemiology of chancroid.

Full text

PDF
1949

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altwegg M., Altwegg-Bissig R., Demarta A., Peduzzi R., Reeves M. W., Swaminathan B. Comparison of four typing methods for Aeromonas species. J Diarrhoeal Dis Res. 1988 Jun;6(2):88–94. [PubMed] [Google Scholar]
  2. Altwegg M., Hickman-Brenner F. W., Farmer J. J., 3rd Ribosomal RNA gene restriction patterns provide increased sensitivity for typing Salmonella typhi strains. J Infect Dis. 1989 Jul;160(1):145–149. doi: 10.1093/infdis/160.1.145. [DOI] [PubMed] [Google Scholar]
  3. Anderson B., Albritton W. L., Biddle J., Johnson S. R. Common beta-lactamase-specifying plasmid in Haemophilus ducreyi and Neisseria gonorrhoeae. Antimicrob Agents Chemother. 1984 Feb;25(2):296–297. doi: 10.1128/aac.25.2.296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blackmore C. A., Limpakarnjanarat K., Rigau-Pérez J. G., Albritton W. L., Greenwood J. R. An outbreak of chancroid in Orange County, California: descriptive epidemiology and disease-control measures. J Infect Dis. 1985 May;151(5):840–844. doi: 10.1093/infdis/151.5.840. [DOI] [PubMed] [Google Scholar]
  5. Brenner D. J., McWhorter A. C., Knutson J. K., Steigerwalt A. G. Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. J Clin Microbiol. 1982 Jun;15(6):1133–1140. doi: 10.1128/jcm.15.6.1133-1140.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grimont F., Grimont P. A. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur Microbiol. 1986 Sep-Oct;137B(2):165–175. doi: 10.1016/s0769-2609(86)80105-3. [DOI] [PubMed] [Google Scholar]
  7. Irino K., Grimont F., Casin I., Grimont P. A. rRNA gene restriction patterns of Haemophilus influenzae biogroup aegyptius strains associated with Brazilian purpuric fever. J Clin Microbiol. 1988 Aug;26(8):1535–1538. doi: 10.1128/jcm.26.8.1535-1538.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Keevil C. W., Spillane B. J., Major N. C. Plasmid stability and antibiotic resistance of Neisseria gonorrhoea during glucose-limited continuous culture. J Med Microbiol. 1987 Dec;24(4):351–357. doi: 10.1099/00222615-24-4-351. [DOI] [PubMed] [Google Scholar]
  9. Korting H. C., Abeck D., Johnson A. P., Ballard R. C., Taylor-Robinson D., Braun-Falco O. Lectin typing of Haemophilus ducreyi. Eur J Clin Microbiol Infect Dis. 1988 Oct;7(5):678–680. doi: 10.1007/BF01964253. [DOI] [PubMed] [Google Scholar]
  10. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Morse S. A. Chancroid and Haemophilus ducreyi. Clin Microbiol Rev. 1989 Apr;2(2):137–157. doi: 10.1128/cmr.2.2.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Odumeru J. A., Ronald A. R., Albritton W. L. Characterization of cell proteins of Haemophilus ducreyi by polyacrylamide gel electrophoresis. J Infect Dis. 1983 Oct;148(4):710–714. doi: 10.1093/infdis/148.4.710. [DOI] [PubMed] [Google Scholar]
  13. Owen R. J., Beck A., Dayal P. A., Dawson C. Detection of genomic variation in Providencia stuartii clinical isolates by analysis of DNA restriction fragment length polymorphisms containing rRNA cistrons. J Clin Microbiol. 1988 Oct;26(10):2161–2166. doi: 10.1128/jcm.26.10.2161-2166.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pennington T. H., Harker C., Thomson-Carter F. Identification of coagulase-negative staphylococci by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and rRNA restriction patterns. J Clin Microbiol. 1991 Feb;29(2):390–392. doi: 10.1128/jcm.29.2.390-392.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Roberts M., Elwell L. P., Falkow S. Molecular characterization of two beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae. J Bacteriol. 1977 Aug;131(2):557–563. doi: 10.1128/jb.131.2.557-563.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rossau R., Duhamel M., Jannes G., Decourt J. L., Van Heuverswyn H. The development of specific rRNA-derived oligonucleotide probes for Haemophilus ducreyi, the causative agent of chancroid. J Gen Microbiol. 1991 Feb;137(2):277–285. doi: 10.1099/00221287-137-2-277. [DOI] [PubMed] [Google Scholar]
  17. Slootmans L., Vanden Berghe D. A., Piot P. Typing Haemophilus ducreyi by indirect immunofluorescence assay. Genitourin Med. 1985 Apr;61(2):123–126. doi: 10.1136/sti.61.2.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Southern E. M. Measurement of DNA length by gel electrophoresis. Anal Biochem. 1979 Dec;100(2):319–323. doi: 10.1016/0003-2697(79)90235-5. [DOI] [PubMed] [Google Scholar]
  19. Stull T. L., LiPuma J. J., Edlind T. D. A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis. 1988 Feb;157(2):280–286. doi: 10.1093/infdis/157.2.280. [DOI] [PubMed] [Google Scholar]
  20. Sturm A. W., Stolting G. J., Cormane R. H., Zanen H. C. Clinical and microbiological evaluation of 46 episodes of genital ulceration. Genitourin Med. 1987 Apr;63(2):98–101. doi: 10.1136/sti.63.2.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Taylor D. N., Echeverria P., Hanchalay S., Pitarangsi C., Slootmans L., Piot P. Antimicrobial susceptibility and characterization of outer membrane proteins of Haemophilus ducreyi isolated in Thailand. J Clin Microbiol. 1985 Mar;21(3):442–444. doi: 10.1128/jcm.21.3.442-444.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Thompson R., Hughes S. G., Broda P. Plasmid identification using specific endonucleases. Mol Gen Genet. 1974;133(2):141–149. doi: 10.1007/BF00264835. [DOI] [PubMed] [Google Scholar]
  23. Van Dyck E., Piot P. Enzyme profile of Haemophilus ducreyi strains isolated on different continents. Eur J Clin Microbiol. 1987 Feb;6(1):40–43. doi: 10.1007/BF02097188. [DOI] [PubMed] [Google Scholar]
  24. Yogev D., Halachmi D., Kenny G. E., Razin S. Distinction of species and strains of mycoplasmas (mollicutes) by genomic DNA fingerprints with an rRNA gene probe. J Clin Microbiol. 1988 Jun;26(6):1198–1201. doi: 10.1128/jcm.26.6.1198-1201.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES